New cluster of ribosomal genes in Bacillus subtilis with regulatory role in sporulation (original) (raw)

Nature volume 272, pages 179–181 (1978)Cite this article

Abstract

STUDIES of the onset of sporulation in Bacillus subtilis can provide information of value to the study of differentiation in general. Little is known about the molecular mechanisms involved in initiating sporulation, or any other developmental process. Mutations at any one of nine distinct genetic loci may block sporulation in B. subtilis at early stages without affecting vegetative growth1,2. These mutations are pleiotropic, in that they are not only blocked in sporulation but are also impaired, to various degrees, in stage 0 functions such as antibiotic and protease production, competence for transformation, and sensitivity to surface active antibiotics and phage3. We have examined the ribosomal composition of these stage 0 mutants and of the same mutants bearing an additional mutation in another locus, abrB, that results in suppression of the pleiotropic mutant phenotype without suppressing the sporulation defect. We report here that abrB mutations define a new cluster of ribosomal protein genes, with the origin proximal to the main group of ribosomal genes. These findings demonstrate the involvement of ribosomes in the control of early stages of the sporulation cycle.

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References

  1. Hoch, J. A. Adv. Genet. 18, 69–99 (1976).
    Article CAS Google Scholar
  2. Piggot, P. J. & Coote, J. G. Bact. Rev. 40, 908–962 (1976).
    CAS PubMed Google Scholar
  3. Brehm, S. P., Staal, S. P. & Hoch, J. A. J. Bact. 115, 1063–1070 (1973).
    CAS PubMed Google Scholar
  4. Guespin-Michel, J. F. Molec. gen. Genet. 112, 243–254 (1971).
    CAS PubMed Google Scholar
  5. Ito, J. Molec. gen. Genet. 124, 97–106 (1973).
    Article CAS Google Scholar
  6. Young, F. E. & Wilson, G. A. in Spores Vol. 6 (eds Gerhardt, P., Costilow, R. N. & Sadoff, H. L.) 596–614 (American Society for Microbiology, Washington, D.C., (1975).
    Google Scholar
  7. Domoto, T., Kobayashi, K. & Kobayashi, Y. in Spores Vol. 6 (eds Gerhardt, P.. Costilow, R. N. & Sadoff, H. L.), 307–313 (American Society for Microbiology, Washington, D.C., (1975).
    Google Scholar
  8. Fortnagel, P. & Bergman, R. Biochim. biophys. Acta 299, 136–141 (1973).
    Article CAS Google Scholar
  9. Leighton, T. J. J. molec. Biol. 86, 855–863 (1974).
    Article CAS Google Scholar
  10. Rhaese, H.-J., Hoch, J. A. & Groscurth, R. Proc. natn. Acad. Sci. U.S.A. 74, 1125–1129 (1977).
    Article ADS CAS Google Scholar
  11. Harford, N. et al. in Microbiology—1976 (ed. Schlessinger, D.) 28–34 (American Society for Microbiology, Washington, D.C., (1976).
    Google Scholar
  12. Freienstein, C. & Blobel, G. Proc. natn. Acad. Sci. U.S.A. 72, 3392–3396 (1975).
    Article ADS CAS Google Scholar
  13. Martini, O. H. W. & Gould, H. J. J. molec. Biol. 62, 403–405 (1971).
    Article CAS Google Scholar
  14. Geisser, M., Tischendorf, G. W. & Stöffler, G. Molec. gen. Genet. 127, 129–145 (1973).
    Article CAS Google Scholar

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Authors and Affiliations

  1. Department of Microbiology, Scripps Clinic and Research Foundation, La Jolla, California, 92037
    JOHN TROWSDALE, MAUREEN SHIFLETT & JAMES A. HOCH

Authors

  1. JOHN TROWSDALE
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  2. MAUREEN SHIFLETT
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  3. JAMES A. HOCH
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TROWSDALE, J., SHIFLETT, M. & HOCH, J. New cluster of ribosomal genes in Bacillus subtilis with regulatory role in sporulation.Nature 272, 179–181 (1978). https://doi.org/10.1038/272179a0

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