Isolation of the human placenta receptor for epidermal growth factor-urogastrone (original) (raw)

Nature volume 277, pages 403–405 (1979)Cite this article

Abstract

EPIDERMAL growth factor-urogastrone (EGF-URO), a singlechain polypeptide of molecular weight ∼6,000, found both in the mouse1–3 and in man4–7, is both a potent stimulant of cell proliferation and an inhibitor of gastric acid secretion. Specific membrane receptors for EGF-URO can be detected in various tissues including human placenta8–11. Although the mouse and human polypeptide sequences differ at 16 positions, they can share the same receptor site in human tissues3,12. There has been considerable recent interest in EGF-URO and its receptor, not only because of the potent mitogenic and acid-inhibitory actions of the polypeptide, but also because of the association of viral13, chemical14 and spontaneous15 cell transformation with a specific reduction in cell receptors for EGF-URO. It is also believed that EGF-URO and its receptor may be involved in the development, maintenance, and differentiation of tissues as diverse as the lens16, the palate17 and the keratin layer of skin18. In experiments aimed at the isolation of the membrane receptor for EGF-URO, we have observed that, unlike the receptor for insulin19, but like the muscarinic receptor for acetylcholine20, the EGF-URO receptor loses its ligand recognition property following solubilisation with detergents. We therefore developed an affinity-labelling technique using glutaraldehyde, that can covalently crosslink the receptor with 125I-labelled EGF-URO before solubilisation21. Independently, Das and coworkers have developed a photoaffinity-labelling technique that can also be used to radiolabel the EGF-URO receptor before solubilisation22. Here we report the success of both our previously described glutaraldehyde crosslinking technique21 (using 125I-EGF-URO for receptor labelling) and of a newly developed photoaffinity-labelling technique (using a photolabile 125I-EGF-URO crosslinking reagent) to identify the EGF-URO receptor in human placenta membranes and to effect the isolation of the affinity-labelled receptor by affinity chromatography using lectin and murine EGF-URO antibody–Agarose columns.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

References

  1. Cohen, S. J. biol. Chem. 237, 1555–1562 (1962).
    CAS PubMed Google Scholar
  2. Cohen, S. J. invest. Derm. 59, 13–16 (1972).
    Article CAS PubMed Google Scholar
  3. Cohen, S. & Carpenter, G. Proc. natn. Acad. Sci. U.S.A. 72, 1317–1321 (1975).
    Article ADS CAS Google Scholar
  4. Gregory, H. Nature 257, 325–327 (1975).
    Article ADS CAS PubMed Google Scholar
  5. Gregory, H. & Preston, B. M. Int. J. Peptide Protein Res. 9, 107–118 (1977).
    Article CAS Google Scholar
  6. Gregory, H. & Willshire, I. R. Hoppe-Seylers' Z. physiol. Chem. 356, 1765–1774 (1975).
    Article CAS Google Scholar
  7. Hollenberg, M. D. Pan American Association of Biochemical Societies Symposium, 5, 265–268 (Academic, New York, 1976).
    Google Scholar
  8. Hollenberg, M. D. & Cuatrecasas, P. Proc. natn. Acad. Sci. U.S.A. 70, 2964–2968 (1973).
    Article ADS CAS Google Scholar
  9. O'Keefe, E., Hollenberg, M. D. & Cuatrecasas, P. Archs Biochem. Biophys. 164, 518–526 (1974).
    Article CAS Google Scholar
  10. Hollenberg, M. D. & Cuatrecasas, P. J. biol. Chem. 250, 3845–3853 (1975).
    CAS PubMed Google Scholar
  11. Carpenter, G., Lembach, K. J., Morrison, M. M. & Cohen, S. J. biol. Chem. 250, 4297–4304 (1975).
    CAS PubMed Google Scholar
  12. Hollenberg, M. D. & Gregory, H. Life Sci. 20, 267–274 (1976).
    Article Google Scholar
  13. Todaro, G. J., DeLarco, J. E. & Cohen, S. Nature 264, 26–31 (1976).
    Article ADS CAS PubMed Google Scholar
  14. Hollenberg, M. D., Barrett, I. C., Tso, P. O. P. & Berhanu, P. Cancer Res. (submitted).
  15. Pratt, R. M. & Pastan, I. Nature 272, 68–70 (1978).
    Article ADS CAS PubMed Google Scholar
  16. Hollenberg, M. D. Archs Biochem. Biophys. 171, 371–377 (1975).
    Article CAS Google Scholar
  17. Hassell, J. R. & Pratt, R. M. Expl. Cell Res. 106, 55–62 (1977).
    Article CAS Google Scholar
  18. Rheinwald, J. G. & Green, H. Nature 265, 421–424 (1977).
    Article ADS CAS PubMed Google Scholar
  19. Cuatrecasas, P. Fedn. Proc. 32, 1838–1846 (1973).
    CAS Google Scholar
  20. Fewtrell, C. M. S. & Rang, H. P. in Drug Receptors, (ed. Rang, H. P.) 211–224 (University Park Press, Baltimore, 1973).
    Book Google Scholar
  21. Sahyoun, N., Hock, R. A. & Hollenberg, M. D. Proc. natn. Acad. Sci. U.S.A. 75, 1675–1679 (1978).
    Article ADS CAS Google Scholar
  22. Das, M., Miyakawa, T., Fox, C. F., Pruss, R., Aharonov, A., Herschman, H. R. Proc. natn. Acad. Sci. U.S.A. 74, 2790–2794 (1977).
    Article ADS CAS Google Scholar
  23. Hock, R. A. & Hollenberg, M. D. Clin. Res. 25, 665A (1977).
    Google Scholar
  24. Carpenter, G. & Cohen, S. Biochem. biophys. Res. Commun. 79, 545–552 (1977).
    Article CAS PubMed Google Scholar
  25. Atlas, D. & Levitzki, A. Nature 272, 370–371 (1978).
    Article ADS CAS PubMed Google Scholar
  26. Das, M. & Fox, C. F. Proc. natn. Acad. Sci. U.S.A. 75, 2644–2648 (1978).
    Article ADS CAS Google Scholar
  27. Laemmli, U., Nature 272, 370–371 (1978).
    Article Google Scholar
  28. March, S. C., Parikh, I. & Cuatrecasas, P. Analyt. Biochem. 60, 149–152 (1974).
    Article CAS PubMed Google Scholar
  29. Spivak, J. L., Small, D. & Hollenberg, M. D. Proc. natn. Acad. Sci. U.S.A. 74, 4633–4635 (1977).
    Article ADS CAS Google Scholar

Download references

Author information

Authors and Affiliations

  1. Howard Hughes Medical Institute, Division of Clinical Pharmacology, Department of Medicine and Department of Pharmacology and Experimental Therapeutics, The Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205
    R. ALAN HOCK, EBBA NEXØ & MORLEY D. HOLLENBERG

Authors

  1. R. ALAN HOCK
    You can also search for this author inPubMed Google Scholar
  2. EBBA NEXØ
    You can also search for this author inPubMed Google Scholar
  3. MORLEY D. HOLLENBERG
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

HOCK, R., NEXØ, E. & HOLLENBERG, M. Isolation of the human placenta receptor for epidermal growth factor-urogastrone.Nature 277, 403–405 (1979). https://doi.org/10.1038/277403a0

Download citation