Histone gene deficiencies and position–effect variegation in Drosophila (original) (raw)

Nature volume 282, pages 312–314 (1979)Cite this article

Abstract

Position–effect variegation, originally observed in insects, has since been demonstrated in plants and mammals. When cells bear a chromosomal rearrangement which juxtaposes a euchromatic gene near to heterochromatin, a fraction of the cells exhibit no expression of that gene. The resultant organism is a mosaic for activity of the rearranged gene. The degree of mosaicism can be modified; such factors as elevated developmental temperature, additional Y chromosome heterochromatin in the genome, and various modifier genes enhance the proportion of cells in which the gene is active1. Although the phenomenon has been extensively documented, the underlying molecular mechanism of position–effect variegation remains unclear. The proportion of cells exhibiting gene inactivation increases with greater proximity of the rearranged gene locus to heterochromatin. It has been proposed that the variegating gene is inactivated by a ‘spreading effect’, or limited spatial diffusion of molecules from the adjacent heterochromatin2. This explanation supposes that the juxtaposed gene locus is condensed into a transcriptionally inactive state, possibly by a phosphorylated histone protein subspecies characteristic of Drosophila heterochromatin3. Such a model leads to the prediction that an alteration in the amount of cellular histone protein would affect the expression of variegating genes. To test this prediction, we have investigated the effect of a reduction in the number of genes coding for histone protein on the degree of mosaicism observed in two variegating gene systems. Our results suggest that the amount of variegating gene expression is a function of histone gene multiplicity.

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Author notes

  1. David P. Cross
    Present address: Department of Neurosciences, McMaster University, Hamilton, Ontario, Canada

Authors and Affiliations

  1. Department of Zoology, The University of British Columbia, Vancouver, British Columbia, Canada, V6T 1W5
    Gerald D. Moore, James D. Procunier, David P. Cross & Thomas A. Grigliatti

Authors

  1. Gerald D. Moore
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  2. James D. Procunier
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  3. David P. Cross
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  4. Thomas A. Grigliatti
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Moore, G., Procunier, J., Cross, D. et al. Histone gene deficiencies and position–effect variegation in Drosophila.Nature 282, 312–314 (1979). https://doi.org/10.1038/282312a0

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