Aberrant rearrangement of the κ light-chain locus involving the heavy-chain locus and chromosome 15 in a mouse plasmacytoma (original) (raw)

Nature volume 301, pages 425–427 (1983)Cite this article

Abstract

The creation of a functional antibody gene requires the precise recombination of gene segments initially separated on the chromosome. Frequently errors occur in the process, resulting in the formation of a non-functional gene. The non-functional genes can be generated by incomplete rearrangements1, frame-shifts2, or the use of pseudo V or J joining segments3. It is likely that these aberrant rearrangements arise by the same mechanism as is used in generating functional genes, a process which we have suggested may involve unequal sister chromatid exchange4. Aberrant rearrangements of immunoglobulin genes occur in normal lymphocytes and play a major part in allelic exclusion5. However, it has recently been suggested that aberrant rearrangements involving immunoglobulin and non-immunoglobulin genes may be involved in tumorigenesis. This suggestion has been stimulated by the frequent occurrence of translocations involving chromosomes known to carry immunoglobulin genes in B-cell malignancies. The rearrangement of non-immunoglobulin DNA to the heavy-chain locus has recently been reported6,7. Some aberrant rearrangements of the κ locus appear to be due to rearrangements to sites that do not include the conventional sequence for V gene segment joining8. Here we describe an aberrant κ rearrangement that has led to the joining of DNA from chromosomes 15, 6 and 12, and so appears to be the result of chromosomal translocations or transpositions. As 15/6 or 15/12 translocations have frequently been found in mouse plasmacytomas (as have analogous translocations in human lymphocyte tumours) this aberrant κ rearrangement may be unique to the plasmacytoma from which it was isolated.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

References

  1. Sakano, H., Kurosawa, Y., Weigert, M. & Tonegawa, S. Nature 290, 562–565 (1981).
    Article ADS CAS Google Scholar
  2. Max, E. E. et al. Cell 21, 793–798 (1980).
    Article CAS Google Scholar
  3. Seidman, J. G. & Leder, P. Nature 286, 779 (1980).
    Article ADS CAS Google Scholar
  4. VanNess, B. G., Coleclough, C., Perry, R. P. & Weigert, M. Proc. natn. Acad. Sci. U.S.A. 79, 262–266 (1982).
    Article ADS CAS Google Scholar
  5. Coleclough, C., Perry, R. P., Karjalainen, K. & Weigert, M. Nature 290, 372–378 (1981).
    Article ADS CAS Google Scholar
  6. Kirsch, I. R. et al. Nature 293, 585–587 (1981).
    Article ADS CAS Google Scholar
  7. Harris, L. J., Lang, R. B. & Marcu, K. B. Proc. natn. Acad. Sci. U.S.A. 79, 4175–4179 (1982).
    Article ADS CAS Google Scholar
  8. Leder, P. et al. Cold Spring Harb. Symp. quant. Biol. 45, 859–865 (1980).
    Article Google Scholar
  9. Perry, R. P. et al. Proc. natn. Acad. Sci. U.S.A. 77, 1937–1941 (1980).
    Article ADS CAS Google Scholar
  10. Perry, R. P., Coleclough, C. & Weigert, M. Cold Spring Harb. Symp. quant. Biol. 45, 925–933 (1980).
    Article Google Scholar
  11. Marcu, K. B. et al. Cold Spring Harb. Symp. quant. Biol. 45, 899–911 (1980).
    Article Google Scholar
  12. Nikaido, T., Nakai, S. & Honjo, T. Nature 292, 845–848 (1981).
    Article ADS CAS Google Scholar
  13. Max, E., Maizel, J. & Leder, P. J. biol. Chem. 236, 5116–5120 (1981).
    Google Scholar
  14. Heiter, P. A. et al. Cell 22, 197–207 (1980).
    Article Google Scholar
  15. Ohno, S. et al. Cell 18, 1001–1007 (1979).
    Article CAS Google Scholar
  16. Klein, G. Nature 294, 313–318 (1981).
    Article ADS CAS Google Scholar
  17. Harris, L. J. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
  18. Höchtl, J., Müller, C. R., Zachau, H. G. Proc. natn. Acad. Sci. U.S.A. 79, 1383–1387 (1982).
    Article ADS Google Scholar
  19. Maxam, A. M. & Gilbert, W., Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1979).
    Article ADS Google Scholar

Download references

Author information

Author notes

  1. Brian G. VanNess
    Present address: Department of Biochemistry, University of Iowa, Iowa City, Iowa, 52242, USA

Authors and Affiliations

  1. The Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania, 19111, USA
    Brian G. VanNess, Marjorie Shapiro, Dawn E. Kelley, Robert P. Perry & Martin Weigert
  2. Department of Biochemistry, New York University, New York, 10016, USA
    Peter D'Eustachio
  3. Department of Biology, Yale University, New Haven, Connecticut, 06511, USA
    Frank Ruddle

Authors

  1. Brian G. VanNess
    You can also search for this author inPubMed Google Scholar
  2. Marjorie Shapiro
    You can also search for this author inPubMed Google Scholar
  3. Dawn E. Kelley
    You can also search for this author inPubMed Google Scholar
  4. Robert P. Perry
    You can also search for this author inPubMed Google Scholar
  5. Martin Weigert
    You can also search for this author inPubMed Google Scholar
  6. Peter D'Eustachio
    You can also search for this author inPubMed Google Scholar
  7. Frank Ruddle
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

VanNess, B., Shapiro, M., Kelley, D. et al. Aberrant rearrangement of the κ light-chain locus involving the heavy-chain locus and chromosome 15 in a mouse plasmacytoma.Nature 301, 425–427 (1983). https://doi.org/10.1038/301425a0

Download citation