Two RNA polymerase sigma factors from Bacillus subtilis discriminate between overlapping promoters for a developmentally regulated gene (original) (raw)

Nature volume 302, pages 800–804 (1983)Cite this article

Abstract

A developmentally regulated gene (spoVG) from the spore-forming bacterium Bacillus subtilis is expressed from two overlapping promoters, which direct transcription initiating from sites separated by 10 base pairs. Utilization of the upstream promoter is determined by an RNA polymerase sigma factor of molecular weight 37,000 (σ37). We report the isolation of a 32,000-molecular weight species of sigma factor (σ32), which exclusively dictates transcription initiation from the downstream promoter, and suggest a model for the way in which sigma-specific recognition sequences are intermeshed within the spoVG transcription initiation region.

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Authors and Affiliations

  1. Department of Cellular and Developmental Biology, The Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts, 02138, USA
    W. Charles Johnson, Charles P. Moran Jr & Richard Losick

Authors

  1. W. Charles Johnson
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  2. Charles P. Moran Jr
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  3. Richard Losick
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Johnson, W., Moran, C. & Losick, R. Two RNA polymerase sigma factors from Bacillus subtilis discriminate between overlapping promoters for a developmentally regulated gene.Nature 302, 800–804 (1983). https://doi.org/10.1038/302800a0

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