Formation of the 3′ end of histone mRNA by post-transcriptional processing (original) (raw)
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- Published: 08 March 1984
Nature volume 308, pages 203–206 (1984)Cite this article
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Abstract
The specific 3′ termini of a number of eukaryotic mRNAs have been shown to be generated by the post-transcriptional processing of primary transcripts or pre-mRNAs1–4. The sequence AAUAAA, present in the 3′ region of nearly all eukaryotic mRNAs, appears to be involved in the cleavage and subsequent polyadenylation of the primary transcript5. An exception to this general rule is the case of the histone mRNAs, which lack the AAUAAA sequence and are not normally polyadenylated. Histone mRNAs do, however, contain a highly conserved 23 base pair sequence at their 3′ termini6, which is required for correct 3′ end formation7. The similarity between this conserved sequence, which can be drawn as a hairpin loop, and bacterial transcription terminators8 has led several investigators to suggest that the specific 3′ end of histone mRNA is formed by termination of transcription9,10. So far, however, experimental results have not been presented which make it possible to distinguish between a post-transcriptional processing or a transcription termination mechanism for the formation of histone mRNA 3′ termini. We have investigated this issue by synthesizing in vitro unprocessed histone pre-mRNAs that extend past the normal 3′ terminus. These in vitro synthesized pre-mRNAS were injected into frog oocyte nuclei to study their fate. The results demonstrate that correct 3′ ends of chicken histone H2B mRNAs can be formed by RNA processing of longer synthetic pre-mRNAs.
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References
- Ford, J. & Hsu, M. J. Virol. 28, 795–801 (1978).
CAS PubMed PubMed Central Google Scholar - Nevins, J. R. & Darnell, J. E. Cell 15, 1477–1493 (1978).
Article CAS PubMed Google Scholar - Hofer, E. & Darnell, J. E. Cell 23, 585–593 (1981).
Article CAS PubMed Google Scholar - Manley, J. L., Sharp, P. A. & Gefter, M. L. J. molec. Biol. 159, 581–589 (1982).
Article CAS PubMed Google Scholar - Fitzgerald, M. & Shenk, T. Cell 24, 251–260 (1981).
Article CAS PubMed Google Scholar - Hentschel, C. C. & Birnstiel, M. L. Cell 25, 301–313 (1981).
Article CAS PubMed Google Scholar - Birchmeier, C., Grosschedl, R. & Birnstiel, M. L. Cell 28, 739–745 (1982).
Article CAS PubMed Google Scholar - Pribnow, D. in Biological Regulation and Development Vol. 1 (ed. Goldberger, R. F.) 250–277 (Plenum, New York, 1979).
Google Scholar - Busslinger, M., Portmann, R. & Birnstiel, M. L. Nucleic Acids Res. 6, 2997–3008 (1979).
Article CAS PubMed PubMed Central Google Scholar - Stunnenberg, H. G. & Birnstiel, M. L. Proc. natn. Acad. Sci. U.S.A. 79, 6201–6204 (1982).
Article ADS CAS Google Scholar - Probst, E., Kressman, A. & Birnstiel, M. L. J. molec. Biol. 135, 709–732 (1979).
Article CAS PubMed Google Scholar - Butler, E. & Chamberlin, M. J. biol. Chem. 257, 5772–5778 (1982).
CAS PubMed Google Scholar - Green, M., Maniatis, T. & Melton, D. Cell 32, 681–694 (1983).
Article CAS PubMed Google Scholar - Harvey, R. P., Robins, A. J. & Wells, J. R. E. Nucleic Acids Res. 10, 7851–7863 (1982).
Article CAS PubMed PubMed Central Google Scholar - Gurdon, J. & Melton, D. A. A. Rev. Genet. 15, 189–218.
- Hentschel, C., Irminger, J., Bucher, P. & Birnstiel, M. L. Nature 285, 147–151 (1980).
Article ADS CAS PubMed Google Scholar - Seiler-Tuyns, A. & Birnstiel, M. L. J. molec. Biol. 151, 607–626 (1981).
Article CAS PubMed Google Scholar - Zernick, M., Heintz, N., Boime, I. & Roeder, R. G. Cell 22, 807–815 (1980).
Article Google Scholar - Turner, P. & Woodland, H. Nucleic Acids Res. 10, 3769–3780 (1982).
Article CAS PubMed PubMed Central Google Scholar - Melton, D., DeRobertis, E. & Cortese, R. Nature 284, 143–148 (1980).
Article ADS CAS PubMed Google Scholar - Melton, D. & Cortese, R. Cell 18, 1165–1172 (1979).
Article CAS PubMed Google Scholar - Galli, G., Hofstetter, H., Stunnenberg, H. & Birnstiel, M. L. Cell 34, 823–828 (1983).
Article CAS PubMed Google Scholar
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Authors and Affiliations
- Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts, 02138, USA
P. A. Krieg & D. A. Melton
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- P. A. Krieg
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Krieg, P., Melton, D. Formation of the 3′ end of histone mRNA by post-transcriptional processing.Nature 308, 203–206 (1984). https://doi.org/10.1038/308203a0
- Received: 25 October 1983
- Accepted: 13 December 1983
- Issue Date: 08 March 1984
- DOI: https://doi.org/10.1038/308203a0