T-cell recognition of a chimaeric class II/class I MHC molecule and the role of L3T4 (original) (raw)
- Letter
- Published: 03 October 1985
- James McCluskey2,
- Terry I. Munitz1,
- Ronald N. Germain2,
- David H. Margulies2 &
- …
- Alfred Singer1
Nature volume 317, pages 425–427 (1985)Cite this article
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Abstract
In addition to expressing clonally distributed antigen-specific and major histocompatibility complex (MHC)-restricted receptors, T cells also express non-clonally distributed surface molecules that are involved in T-cell function. Among the most intriguing of the latter are L3T4 and Lyt 2, which are expressed on individual T lymphocytes in striking, though not absolute, concordance with their restriction by either class II or class I MHC determinants1–5, and which are thought to contribute to the overall avidity of T-cell interactions by binding to monomorphic determinants on class II and class I MHC molecules, respectively1–9. To examine the ability of T cells to recognize a single class II domain in the absence of the remainder of the Ia molecule, as well as to evaluate the structural basis for the putative interaction of L3T4 with Ia, a recombinant class II/class I murine MHC gene was constructed and introduced into mouse L cells10. Here we demonstrate that a subset of class II allospecific cytotoxic T lymphocytes (CTL) can specifically recognize and lyse L-cell transfectants expressing an isolated polymorphic A_β_1 domain, and that anti-L3T4 antibody can block such killing, a result inconsistent with the highly conserved membrane-proximal domains of Ia acting as unique target sites for L3T4 binding.
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References
- Swain, S. L. Proc. natn. Acad. Sci. U.S.A. 78, 7101–7105 (1981).
Article ADS CAS Google Scholar - Reinherz, E. L., Meuer, S. C. & Schlossman, S. F. Immun. Rev. 74, 81–112 (1983).
Article Google Scholar - Swain, S. L. Immun. Rev. 74, 129–142 (1983).
Article CAS PubMed Google Scholar - Dialynas, D. P. et al. J. Immun. 131, 2445–2451 (1983).
CAS PubMed Google Scholar - Biddison, W. E., Rao, P. E., Talle, M. A., Goldstein, G. & Shaw, S. J. exp. Med. 156, 1065–1076 (1982).
Article CAS PubMed Google Scholar - Marrack, P. et al. J. exp. Med. 158, 1077–1091 (1983).
Article CAS PubMed Google Scholar - Greenstein, J.L., Kappler, J., Marrack, P. & Burakoff, S.J. J. exp. Med. 159, 1213–1224 (1984).
Article CAS PubMed Google Scholar - Biddison, W. E., Rao, P. E., Talle, M. A., Goldstein, G. & Shaw, S. J. exp. Med. 159, 783–79 (1984).
Article CAS PubMed Google Scholar - Swain, S. L., Dialynas, D., Fitch, F. W. & English, M. J. Immun. 132, 1118–1123 (1984).
CAS PubMed Google Scholar - McCluskey, J., Germain, R. N. & Margulies, D. H. Cell 40, 247–257 (1985).
Article CAS PubMed Google Scholar - Golding, H. & Singer, A. J. Immun. 135, 1610–1615 (1985).
CAS PubMed Google Scholar - Germain, R. N. et al. Proc. natn. Acad. Sci. U.S.A. 82, 2940–2944 (1985).
Article ADS CAS Google Scholar - Grimm, E. A. et al. J. exp. Med. 157, 884–897 (1983).
Article CAS PubMed PubMed Central Google Scholar - McCluskey, J., Bluestone, J. A., Coligan, J. E., Maloy, W. L. & Margulies, D. H., J. Immun. (in the press).
- Haas, W. & von Boehmer, H. Eur. J. Immun. 14, 383–384 (1984).
Article CAS Google Scholar - Bluestone, J. A. & Hodes, R. J. Immun. Today 4, 256–258 (1984).
Article Google Scholar - Bekoff, M., Kakiuchi, T. & Grey, H. M. J. Immun. 134, 1337–1342 (1985).
CAS PubMed Google Scholar - Wassmer, P., Chan, C., Logdberg, L. & Shevach, E. J. Immun. (in the press).
- Tite, J. P., Sloan, A. & Janeway, C. A. J. molec. Cell. Immun. (in the press).
- Oi, V. T., Jones, P. P., Goding, J. W., Herzenberg, L. A. & Herzenberg, L. A. Curr. Topics Microbiol. Immun. 7, 115–129 (1978).
Google Scholar - Ozato, K., Hansen, T. H. & Sachs, D. H. J. Immun. 125, 2473–2477 (1980).
CAS PubMed Google Scholar - Pierres, A. et al. J. Immun. 132, 2775–2782 (1984).
CAS PubMed Google Scholar
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Authors and Affiliations
- Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20205, USA
Hana Golding, Terry I. Munitz & Alfred Singer - Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20205, USA
James McCluskey, Ronald N. Germain & David H. Margulies
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Golding, H., McCluskey, J., Munitz, T. et al. T-cell recognition of a chimaeric class II/class I MHC molecule and the role of L3T4.Nature 317, 425–427 (1985). https://doi.org/10.1038/317425a0
- Received: 28 June 1985
- Accepted: 31 July 1985
- Issue Date: 03 October 1985
- DOI: https://doi.org/10.1038/317425a0