T-cell recognition of a chimaeric class II/class I MHC molecule and the role of L3T4 (original) (raw)

Nature volume 317, pages 425–427 (1985)Cite this article

Abstract

In addition to expressing clonally distributed antigen-specific and major histocompatibility complex (MHC)-restricted receptors, T cells also express non-clonally distributed surface molecules that are involved in T-cell function. Among the most intriguing of the latter are L3T4 and Lyt 2, which are expressed on individual T lymphocytes in striking, though not absolute, concordance with their restriction by either class II or class I MHC determinants1–5, and which are thought to contribute to the overall avidity of T-cell interactions by binding to monomorphic determinants on class II and class I MHC molecules, respectively1–9. To examine the ability of T cells to recognize a single class II domain in the absence of the remainder of the Ia molecule, as well as to evaluate the structural basis for the putative interaction of L3T4 with Ia, a recombinant class II/class I murine MHC gene was constructed and introduced into mouse L cells10. Here we demonstrate that a subset of class II allospecific cytotoxic T lymphocytes (CTL) can specifically recognize and lyse L-cell transfectants expressing an isolated polymorphic A_β_1 domain, and that anti-L3T4 antibody can block such killing, a result inconsistent with the highly conserved membrane-proximal domains of Ia acting as unique target sites for L3T4 binding.

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Authors and Affiliations

  1. Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20205, USA
    Hana Golding, Terry I. Munitz & Alfred Singer
  2. Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20205, USA
    James McCluskey, Ronald N. Germain & David H. Margulies

Authors

  1. Hana Golding
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  2. James McCluskey
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  3. Terry I. Munitz
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  4. Ronald N. Germain
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  5. David H. Margulies
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  6. Alfred Singer
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Golding, H., McCluskey, J., Munitz, T. et al. T-cell recognition of a chimaeric class II/class I MHC molecule and the role of L3T4.Nature 317, 425–427 (1985). https://doi.org/10.1038/317425a0

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