‘Brain-specific’ transcription and evolution of the identifier sequence (original) (raw)

Nature volume 319, pages 418–420 (1986)Cite this article

Abstract

A recent model for the transcriptional control of gene expression in neural cells involves a dispersed repetitive DNA sequence termed the identifier (ID) sequence1. However, the model is based on circumstantial evidence from studies on rat brain gene expression2–4. Furthermore, available data are complicated by observations from several laboratories which suggest that the ID sequence is a family of mobile genetic elements5–10. Although this does not preclude a role for some family members in regulating gene expression, the contention that these sequences are transcribed tissue-specifically (refs 1–4, but see ref. 11) is not proof of such a role12. We have now measured the genomic copy number and tissue pattern of transcription of ID sequences in the rat, mouse and hamster, and have found that ID-homologous, BC1-like2 RNAs are restricted to brain in all three species, but that ID-homologous transcripts occur in total cellular RNAs of brain, liver and kidney of all three organisms. The genomic copy number of the ID sequences varies over two orders of magnitude between these species. Our data suggest that most ID sequences in these genomes are dispersed at random with respect to transcription units. A _cis_-acting, transcriptional-level controlling role for the ID therefore seems unlikely.

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References

  1. Sutcliffe, J. G., Milner, R. J., Gottesfeld, J. M. & Reynolds, W. Science 225, 1308–1315 (1984).
    Article ADS CAS Google Scholar
  2. Sutcliffe, J. G., Miller, R. J., Bloom, F. E. & Lerner, R. A. Proc. natn. Acad. Sci. U.S.A. 79, 4942–4946 (1982).
    Article ADS CAS Google Scholar
  3. Milner, R. J., Bloom, F. E., Lai, C., Lerner, R. A. & Sutcliffe, J. G. Proc. natn. Acad. Sci. U.S.A. 81, 713–717 (1984).
    Article ADS CAS Google Scholar
  4. Sutcliffe, J. G., Milner, R. J., Gottesfeld, J. M. & Lerner, R. A. Nature 308, 237–241 (1984).
    Article ADS CAS Google Scholar
  5. Barta, A., Richards, R. I., Baxter, J. D. & Shine, J. Proc. natn. Acad. Sci. U.S.A. 78, 4867–4871 (1981).
    Article ADS CAS Google Scholar
  6. Schuler, R. A., Weber, J. L. & Gorski, J. Nature 305, 159–160 (1983).
    Article ADS CAS Google Scholar
  7. Lemischka, I. & Sharp, P. A. Nature 300, 330–335 (1982).
    Article ADS CAS Google Scholar
  8. Watanabe-Nagasu, N. et al. Nucleic Acids Res. 11, 1791–1801 (1983).
    Article CAS Google Scholar
  9. Hastie, N. Trends Genet. 1, 37 (1985).
    Article CAS Google Scholar
  10. Page, G. L., Smith, S. & Goodman, H. M. Nucleic Acids Res. 9, 2087–2103 (1981).
    Article CAS Google Scholar
  11. Owens, G. P., Chaudhari, N. & Hahn, W. E. Science 229, 1263–1265 (1985).
    Article ADS CAS Google Scholar
  12. Davidson, E. H. & Posakony, J. W. Nature 297, 633–635 (1982).
    Article ADS CAS Google Scholar
  13. Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning, a Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).
    Google Scholar
  14. Chikaraishi, D. Biochemistry 18, 3249–3258 (1979).
    Article CAS Google Scholar
  15. Van Ness, J., Maxwell, J. H. & Hahn, W. E. Cell 18, 1341–1349 (1979).
    Article CAS Google Scholar
  16. Hahn, W. E., Chaudhari, N., Beck, L., Wilber, K. & Peffley, D. Cold Spring Harb. Symp. quant. Biol 48, 465–475 (1983).
    Article CAS Google Scholar
  17. Haynes, J. R. & Jelinek, W. R. Proc. natn. Acad. Sci. U.S.A. 78, 6130–6134 (1981).
    Article ADS CAS Google Scholar
  18. Sargeant, T. D. et al. Proc. natn. Acad. Sci. U.S.A. 76, 3256–3260 (1979).
    Article ADS Google Scholar
  19. Ullu, E. & Tschudi, C. Nature 312, 171–172 (1984).
    Article ADS CAS Google Scholar
  20. Scott, M. P. et al. Cell 35, 763–776 (1983).
    Article CAS Google Scholar
  21. Kafatos, F. C., Jones, C. W. & Efstratiadis, A. Nucleic Acids Res. 7, 1541–1552 (1979).
    Article CAS Google Scholar

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  1. Department of Biology and Centre for Human Genetics, McGill University, 1205 avenue Docteur Penfield, Montreal, Quebec, Canada, H3A 1B1
    Carmen Sapienza & Benoit St-Jacques

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  1. Carmen Sapienza
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  2. Benoit St-Jacques
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Sapienza, C., St-Jacques, B. ‘Brain-specific’ transcription and evolution of the identifier sequence.Nature 319, 418–420 (1986). https://doi.org/10.1038/319418a0

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