Recombinant human TNF induces production of granulocyte–monocyte colony-stimulating factor (original) (raw)

Nature volume 323, pages 79–81 (1986)Cite this article

Abstract

Tumour necrosis factor (TNF) is synthesized by macrophages exposed to endotoxin1. It produces haemorrhagic necrosis of a variety of tumours in mice and is cytostatic or cytocidal against various transformed cell lines in vitro, but viability of normal human or rodent cells is unaffected2–4. The role of TNF is unlikely to be restricted to the rejection of tumours. Colony-stimulating factors (CSFs) are required for survival, proliferation and differentiation of haematopoietic progenitor cells. The haematopoietic growth factor known as granulocyte-monocyte colony-stimulating factor (GM-CSF) has the ability to stimulate proliferation and differentiation of normal granulocyte-monocyte and eosinophil stem cells and enhance the proliferation of pluripotent, megakaryocyte and erythroid stem cells5. In addition, GM-CSF stimulates a variety of functional activities in mature granulocytes and macrophages, for example inhibition of migration, phagocytosis of microbes, oxidative metabolism, and antibody-dependent cytotoxic killing of tumour cells5–7. We show here that TNF markedly stimulates production of GM-CSF messenger RNA and protein in normal human lung fibroblasts and vascular endothelial cells, and in cells of several malignant tissues.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

References

  1. Carswell, E. A. et al. Proc. natn. Acad. Sci. U.S.A. 72, 3666–3670 (1975).
    Article ADS CAS Google Scholar
  2. Helson, L., Green, J., Carswell, E. & Old, L. Nature 258, 730–732 (1975).
    Article ADS Google Scholar
  3. Matthews, N., Watkins, J. F. Br. J. Cancer 38, 302–309 (1978).
    Article CAS PubMed PubMed Central Google Scholar
  4. Aggarwal, B. et al. science 260, 2345–2354 (1985).
    CAS Google Scholar
  5. Metcalf, D. Science 229, 16–22 (1984).
    Article ADS Google Scholar
  6. Gasson, J. et al. science 226, 1339–1342 (1984).
    Article ADS CAS PubMed Google Scholar
  7. Weisbart, R., Golde, D., Clark, S., Wong, G. & Gasson, J. Nature 314, 361–362 (1985).
    Article ADS CAS PubMed Google Scholar
  8. Koeffler, H.P. & Golde, D. Science 200, 1153–1155 (1978).
    Article ADS CAS PubMed Google Scholar
  9. Leary, A., Ogawa, M., Strauss, L. & Civin, C. J. clin. Invest. 74, 2193–2197 (1984).
    Article CAS PubMed PubMed Central Google Scholar
  10. Wong, G. G. et al. science 228, 810–814 (1985).
    Article ADS CAS PubMed Google Scholar
  11. Beutler, B., Mahoney, J., Le Trang, N., Pekala, P. & Cerami, A. J. exp. Med. 161, 984–995 (1985).
    Article CAS PubMed Google Scholar
  12. Collins, T., La Pierre, L. A., Fiers, W., Strominger, J. & Pober, J. S. Proc. natn. Acad. Sci. U.S.A. 83, 446–450 (1986).
    Article ADS CAS Google Scholar
  13. Gamble, J. R., Harlan, J. M., Klebanoff, S. J., Vadas, M. A. Proc. natn. Acad. Sci. U.S.A. 82, 8667–8681 (1985).
    Article ADS CAS Google Scholar
  14. Shalaby, M. R. et al. J. Immun. 135, 2069–2073 (1985).
    CAS PubMed Google Scholar
  15. Sugarman, B. et al. Science 230, 943–945 (1985).
    Article ADS CAS PubMed Google Scholar
  16. Vilcek, J. et al. J. exp. Med. 163, 632–643 (1986).
    Article CAS PubMed Google Scholar
  17. Maniatis, T., Fritsch, E. & Sambrook, J. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).
  18. Souza, L. et al. Science 232, 61 (1986).
    Article ADS CAS PubMed Google Scholar
  19. Zucali, J. R. et al. J. clin. Invest. 77, 1857 (1986).

Download references

Author information

Authors and Affiliations

  1. Department of Medicine, Division of Hematology/Oncology, University of California, Los Angeles Medical Center, Los Angeles, California, 90024, USA
    Reinhold Munker, Judith Gasson & H. Phillip Koeffler
  2. Department of Medicine, Veterans Administration Medical Center, Charleston, South Carolina, 29403, USA
    Makio Ogawa

Authors

  1. Reinhold Munker
    You can also search for this author inPubMed Google Scholar
  2. Judith Gasson
    You can also search for this author inPubMed Google Scholar
  3. Makio Ogawa
    You can also search for this author inPubMed Google Scholar
  4. H. Phillip Koeffler
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Munker, R., Gasson, J., Ogawa, M. et al. Recombinant human TNF induces production of granulocyte–monocyte colony-stimulating factor.Nature 323, 79–81 (1986). https://doi.org/10.1038/323079a0

Download citation

This article is cited by