A c-erb-A binding site in rat growth hormone gene mediates trans-activation by thyroid hormone (original) (raw)
- Letter
- Published: 22 October 1987
- Rodrigo Franco1,2,
- Cary Weinberger3,
- Vivian R. Albert1,
- Ronald M. Evans3,4 &
- …
- Michael G. Rosenfeld1,4
Nature volume 329, pages 738–741 (1987)Cite this article
- 182 Accesses
- 359 Citations
- 6 Altmetric
- Metrics details
Abstract
The substance 3,5,3-triiodothyronine (T3) stimulates growth hormone gene transcription in rat pituitary tumour cells1–4. This stimulation is thought to be mediated by the binding of nuclear T3 receptors to regulatory elements 5' to the transcriptional start site5–8. Understanding of the mechanism by which thyroid hormone activates gene transcription has been limited by failure to purify nuclear T3 receptors because of their low abundance, and by the absence of defined T3 receptor-DNA binding sites affecting T3 regulation. Recently, human and avian c-erb-A gene products have been shown to bind thyroid hormone with high affinity9,10 and to have a molecular weight and nuclear association characteristic of the thyroid hormone receptor. In the present report, we describe the development of an avidin–biotin complex DNA-binding assay which can detect specific, high-affinity binding of rat pituitary cell T3 receptors to the sequence 5'CAGGGACGTGACCGCA3', located 164 base pairs 5' to the transcriptional start site of the rat growth hormone gene. An oligonucleotide containing this sequence transferred T3 regulation to the herpes simplex virus thymidine kinase promoter in transfected rat pituitary GC2 cells, and specifically bound an in vitro translation product of the human placental c-erb-A gene. The data provide supporting evidence that the human c-erb-A gene product mediates the transcriptional effects of T3 and also that GC2 cell nuclear extracts contain additional factors that modify the binding of pituitary T3 receptors to the rat growth hormone gene T3 response element.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
References
- Evans, R. M., Birnberg, N. C. & Rosenfeld, M. G. Proc. natn. Acad. Sci. U.S.A. 79, 7659–7663 (1982).
Article ADS CAS Google Scholar - Diamond, D. J. & Goodman, H. M. J. molec. Biol. 181, 41–62 (1985).
Article CAS Google Scholar - Spindler, S. R., Mellon, S. H. & Baxter, J. D. J. biol. Chem. 257, 11627–11632 (1982).
CAS PubMed Google Scholar - Yaffee, B. M. & Samuels, H. H. J. biol. Chem. 259, 6284–6291 (1984).
Google Scholar - Larsen, P. R., Harney, J. W. & Moore, D. D. J. biol. Chem. 261, 14373–14376 (1986).
CAS PubMed Google Scholar - Flug, F. et al. J. biol. Chem. 262, 6373–6382 (1987).
CAS PubMed Google Scholar - Casanova, J., Copp, R. P., Janocko, L. & Samuels, H. H. J. biol. Chem. 260, 11744–11748 (1985).
CAS PubMed Google Scholar - Crew, M. & Spindler, S. R. J. biol. Chem. 261, 5018–5022 (1986).
CAS PubMed Google Scholar - Sap, J. et al. Nature 324, 635–640 (1986).
Article ADS CAS Google Scholar - Weinberger, C. et al. Nature 324, 641–646 (1986).
Article ADS CAS Google Scholar - Nelson, C. et al. Nature 322, 557–562 (1986).
Article ADS CAS Google Scholar - Elsholtz, H. P. et al. Science 234, 1552–1557 (1986).
Article ADS CAS Google Scholar - West, B. L. et al. Molec. cell. Biol. 7, 1193–1197 (1987).
Article CAS Google Scholar - Catanzaro, D. F., West, B. L., Baxter, J. D. & Reudelhuber, T. L. Molec. Endocr. 1, 90–96 (1987).
Article CAS Google Scholar - Nyborg, J. K. & Spindler, S. R. J. biol. Chem. 261, 5685–5688 (1986).
CAS PubMed Google Scholar - Topol, J., Ruden, D. M. & Parker, C. S. Cell, 42, 527–537 (1985).
Article CAS Google Scholar - Reinberg, D., Horikoshi, M. & Roeder, R. G. J. biol. Chem. 262, 3322–3330 (1987).
CAS PubMed Google Scholar - McKnight, S. & Tjian, R. Cell 46, 795–805 (1986).
Article CAS Google Scholar - Gorman, C. M., Moffat, L. F. & Howard, B. H. Molec. cell. Biol. 2, 1044–1051 (1982).
Article CAS Google Scholar - Linney, E. & Donerly, S. Cell 35, 693–699 (1983).
Article CAS Google Scholar - Kunkel, T. Proc. natn. Acad. Sci. U.S.A. 82, 488–492 (1985).
Article ADS CAS Google Scholar - Dingham, J. D., Lebovitz, R. M. & Roeder, R. G. Nucleic Acids Res. 11, 1475–1489 (1983).
Article Google Scholar - Samuels, H. H., Tsai, J. S., Casanova, J. & Stanley, F. J. clin. Invest. 54, 853–865 (1974).
Article CAS Google Scholar
Author information
Authors and Affiliations
- Centre for Molecular Genetics, Eukaryotic Regulatory Biology Program, School of Medicine, University of California, San Diego, La Jolla, California, 92093, USA
Christopher K. Glass, Rodrigo Franco, Vivian R. Albert & Michael G. Rosenfeld - Department of Biology, Eukaryotic Regulatory Biology Program, School of Medicine, University of California, San Diego, La Jolla, California, 92093, USA
Rodrigo Franco - Salk Institute, La Jolla, California, 92037, USA
Cary Weinberger & Ronald M. Evans - Howard Hughes Medical Institute,
Ronald M. Evans & Michael G. Rosenfeld
Authors
- Christopher K. Glass
You can also search for this author inPubMed Google Scholar - Rodrigo Franco
You can also search for this author inPubMed Google Scholar - Cary Weinberger
You can also search for this author inPubMed Google Scholar - Vivian R. Albert
You can also search for this author inPubMed Google Scholar - Ronald M. Evans
You can also search for this author inPubMed Google Scholar - Michael G. Rosenfeld
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Glass, C., Franco, R., Weinberger, C. et al. A c-erb-A binding site in rat growth hormone gene mediates _trans_-activation by thyroid hormone.Nature 329, 738–741 (1987). https://doi.org/10.1038/329738a0
- Received: 04 June 1987
- Accepted: 28 August 1987
- Issue Date: 22 October 1987
- DOI: https://doi.org/10.1038/329738a0