soluble form of CD4 (T4) protein inhibits AIDS virus infection (original) (raw)

Nature volume 331, pages 82–84 (1988)Cite this article

Abstract

CD4 (T4) is a glycoprotein of relative molecular mass 55,000 (_M_r 55K) on the surface of T lymphocytes which is thought to interact with class II MHC (major histocompatibility complex) molecules, mediating efficient association of helper T cells with antigenbearing targets1–3. The CD4 protein is also the receptor for HIV, a T-lymphotropic RNA virus responsible for the human acquired immune deficiency syndrome (AIDS) (refs 4–7). To define the mechanisms of interaction of CD4 with the surface of antigen-presenting cells and with HIV, we have isolated the CD4 gene and expressed this gene in several different cellular environments7,8. Here we describe an efficient expression system in which a recombinant, soluble form of CD4 (sCD4) is secreted into tissue culture supernatants. This scD4 retains the structural and biological properties of CD4 on the cell surface, binds to the envelope glycoprotein (gpllO) of HIV and inhibits the binding of virus to CD4+ lymphocytes, resulting in a striking inhibition of virus infectivity.

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References

  1. Gay, D. et al. Nature 328, 626–629 (1987).
    Article ADS CAS Google Scholar
  2. Sleckman, B. D. et al. Nature 328, 351–353 (1987).
    Article ADS CAS Google Scholar
  3. Thomas, Y., Rognozinski, L. & Chess, L. Immun. Rev. 74, 113–128 (1985).
    Article Google Scholar
  4. Dalgleish, A. G. et al. Nature 312, 763–766 (1984).
    Article ADS CAS Google Scholar
  5. Klatzman, D. et al. Nature 312, 767–768 (1984).
    Article ADS Google Scholar
  6. McDougal, J. S. et al. Science 231, 382–385 (1986).
    Article ADS CAS Google Scholar
  7. Maddon, P. J. et al. Cell 47, 333–348 (1986).
    Article CAS Google Scholar
  8. Maddon, P. J. et al. Cell 42, 93–104 (1985).
    Article CAS Google Scholar
  9. Maddon, P. J. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
  10. Pfarr, D. S., Sathe, G. & Refi, M. F. DNA 4, 461–467 (1985).
    Article CAS Google Scholar
  11. Urlaud, G., Kas, E., Carothers, A. M. & Chasin, L. A. Cell 33, 405–412 (1983).
    Article Google Scholar
  12. Kaufman, R. J., Sharp, P. A. & Latt, S. A. Molec. cell. Biol. 3, 699–711 (1983).
    Article CAS Google Scholar
  13. McDougal, J.-S. et al. J. immun. Meth. 76, 171–183 (1985).
    Article CAS Google Scholar
  14. Berg, P. E. et al. Molec. cell. Biol. 3, 1246–1254 (1983).
    Article CAS Google Scholar
  15. Subramani, S., Mulligan, R. & Berg, P. Molec. cell. Biol. 1, 854–864 (1981).
    Article CAS Google Scholar
  16. Wigler, M. et al. Cell 11, 223–232 (1977).
    Article CAS Google Scholar
  17. McDougal, J. S. et al. J. Immun. 135, 3151–3162 (1985).
    CAS Google Scholar

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Author notes

  1. Richard Axel: To whom correspondence should be addressed.

Authors and Affiliations

  1. Smith Kline and French Laboratories, King of Prussia, Pennsylvania, 19406, USA
    Keith C. Deen, Richard Inacker, Gail Folena-Wasserman, Jonathan Rosenberg & Raymond W. Sweet
  2. Centers for Disease Control, Atlanta, Georgia, 30333, USA
    J. Steven McDougal
  3. Department of Human Genetics, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, USA
    Jim Arthos
  4. Department of Biochemistry and Molecular Biophysics,
    Paul Jay Maddon
  5. Howard Hughes Medical Institute, Columbia University, 722 West 168th St, New York, New York, 10032, USA
    Richard Axel

Authors

  1. Keith C. Deen
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  2. J. Steven McDougal
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  3. Richard Inacker
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  4. Gail Folena-Wasserman
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  5. Jim Arthos
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  6. Jonathan Rosenberg
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  7. Paul Jay Maddon
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  8. Richard Axel
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  9. Raymond W. Sweet
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Deen, K., McDougal, J., Inacker, R. et al. soluble form of CD4 (T4) protein inhibits AIDS virus infection.Nature 331, 82–84 (1988). https://doi.org/10.1038/331082a0

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