A Harvey-ras responsive transcription element is also responsive to a tumour-promoter and to serum (original) (raw)
- Letter
- Published: 17 March 1988
Nature volume 332, pages 275–278 (1988)Cite this article
- 126 Accesses
- 196 Citations
- Metrics details
Abstract
The ras oncogenes are implicated in the onset of some human tumours, and in cellular proliferation and terminal differentiation. The ras proteins are plasma membrane bound transducers of signals between the outside of the cell and unknown targets in the cell1,2. Identifying these targets and understanding how they are regulated will have a major impact on our understanding of the molecular basis of transformation. We have already shown that c-Ha-ras and the tumor promoter TPA (12-_o_-tetradecanoyl phor-bol-13-acetate) can activate a transcriptional enhancer3. We now report the identification of a short sequence in the polyoma virus (Py) enhancer which mediates Ha-ras activation, and show that this sequence (ras responsive element, RRE) also mediates activation by TPA and serum. This responsive element is a specific binding-site for the mouse transcription factor PEA1 (ref. 4 and below) and for the jun oncogene (ref. 5 and M. Karin, personal communication). These results are in keeping with a role for ras protein in signal transduction from outside the cell to a transcription factor in the nucleus, through protein kinase C1,2. The striking similarity between RRE and DNA sequences present in the promoter regions of a number of transformation-related genes suggests that deregulated activation of RRE is a critical event in transformation.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
References
- Barbacid, M. A. Rev. Biochem. 56, 779–827 (1987).
Article CAS Google Scholar - Micheal, R. & Jackson, T. Nature 328, 668–669 (1987).
Article ADS Google Scholar - Wasylyk, C., Imler, J. L., Perez-Mutul, J. & Wasylyk, B. Cell 48, 525–534 (1987).
Article CAS Google Scholar - Piette, J. & Yaniv, M. EMBO J. 6, 1331–1337 (1987).
Article CAS Google Scholar - Bohmann, D. et al. Science 238, 1386–1392 (1987).
Article ADS CAS Google Scholar - Zenke, M. et al. EMBO, J. 5, 387–397 (1986).
Article CAS Google Scholar - Lacal, J. C., Fleming, T. P., Warran, B. S., Blumberg, P. M. & Aaronson, S. A. Molec. cell. Biol. 7, 4146–4149 (1987).
Article CAS Google Scholar - Lacal, J. C., Moscat, J. & Aaronson, S. A. Nature 330, 269–272 (1987).
Article ADS CAS Google Scholar - Angel, P. et al. Cell 49, 729–739 (1987).
Article CAS Google Scholar - Matrisian, L. M., Glaichenhaus, N., Gesnel, M. C. & Breathnach, R. EMBO J. 4, 1435–1440 (1985).
Article CAS Google Scholar - Lee, W., Mitchell, P. & Tjian, R. Cell 49, 741–752 (1987).
Article CAS Google Scholar - Struhl, K. Cell 50, 841–846 (1987).
Article CAS Google Scholar - Kryszke, M. H., Piette, J. & Yaniv, M. Nature 328, 254–256 (1987).
Article ADS CAS Google Scholar - Veldman, G. M., Lupton, J. & Kamen, R. Molec. cell Biol. 5, 649–658 (1985).
Article CAS Google Scholar - Hassel, J. A., Muller, W. J. & Mueller, C. R. in Cancer Cells, vol. 4 (eds Botchan, M., Grodzickerl, T. & Sharp, P.) (Cold Spring Harbor Laboratory, New York, 1986).
Google Scholar - Herbomel, P., Bourachot, B. & Yaniv, M. Cell 39, 653–662 (1984).
Article CAS Google Scholar - Wildeman, A. G. et al. Molec. cell. Biol. 6, 2098–2105 (1986).
Article CAS Google Scholar - Angel, P., Baumann, I., Stein, B., Delius, H., Rahmsdorf, H. J. & Herrlich, P. Molec. cell. Biol. 7, 2256–2266 (1987).
Article CAS Google Scholar - Fujita, T., Takaoka, C., Matsui, H. & Taniguchi, T. Proc. natn. Acad. Sci. U.S.A. 80, 7437–7441 (1983).
Article ADS CAS Google Scholar
Author information
Authors and Affiliations
- Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, 11 rue Humann, 67085, Strasbourg Cédex, France
J. L. Imler, C. Schatz, C. Wasylyk, B. Chatton & B. Wasylyk
Authors
- J. L. Imler
You can also search for this author inPubMed Google Scholar - C. Schatz
You can also search for this author inPubMed Google Scholar - C. Wasylyk
You can also search for this author inPubMed Google Scholar - B. Chatton
You can also search for this author inPubMed Google Scholar - B. Wasylyk
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Imler, J., Schatz, C., Wasylyk, C. et al. A Harvey-ras responsive transcription element is also responsive to a tumour-promoter and to serum.Nature 332, 275–278 (1988). https://doi.org/10.1038/332275a0
- Received: 16 October 1987
- Accepted: 05 January 1988
- Issue Date: 17 March 1988
- DOI: https://doi.org/10.1038/332275a0