A transgene containing lacZ inserted into the dystonia locus is expressed in neural tube (original) (raw)

Nature volume 335, pages 435–437 (1988)Cite this article

Abstract

The site of integration of transgenes in the host genome can affect levels of expression1 and occasionally confer ectopic patterns of expression on otherwise tissue-specific genes2. We describe here a line of mice in which an hsp68-lacZ transgene is expressed in unstressed developing neural tissue and where the transgene insertion has caused a mutation of a neural tissue-specific gene, dystonia musculorum (dt). This coincidence suggests that expression of the hsp68-lacZ construct may be controlled directly by _cis_-acting regulatory sequences that normally control the developmental expression of the dt gene. Such constructs may serve as useful tools for identifying new tissue-specific enhancers and their associated genes3,4.

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Author notes

  1. R. Kothary
    Present address: Department of Molecular Embryology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK

Authors and Affiliations

  1. Divisionof Molecular and Developmental Biology, Mount Sinai Hospital Research Institute, and Department of Medical Genetics, University of Toronto, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
    R. Kothary, S. Clapoff, A. Brown & J. Rossant
  2. Ludwig Institute for Cancer Research, Royal Victoria Hospital, 687 Avenue des Pins Quest, Montreal, Quebec, H3A 1A1, Canada
    R. Campbell & A. Peterson

Authors

  1. R. Kothary
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  2. S. Clapoff
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  3. A. Brown
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  4. R. Campbell
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  5. A. Peterson
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  6. J. Rossant
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Kothary, R., Clapoff, S., Brown, A. et al. A transgene containing lacZ inserted into the dystonia locus is expressed in neural tube.Nature 335, 435–437 (1988). https://doi.org/10.1038/335435a0

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