Direct interaction between fos and jun nuclear oncoproteins: role of the 'leucine zipper' domain (original) (raw)

Nature volume 336, pages 692–695 (1988)Cite this article

Abstract

Gene expression is modulated by the specific interactions of nuclear proteins with unique regulatory sequences in the genome. Proteins involved in transcriptional regulation seem to be either transcription factors or transcription modulators and their interactions are crucial in determining whether the expression of a specific gene is activated or repressed. Recently1, the product of the proto-oncogene jun has been identified as the transcription factor AP-1, whereas nuclear oncoproteins fos and myc have been implicated in transcriptional transregulation of several promoters2–6. Furthermore, the products of the fos and jun proto-oncogenes are associated in some transcription complexes3,5,7. Although the nature of the association is unclear, the two proteins co-immunoprecipitate with fos antibodies in nuclear extracts8–10. Here, we report studies that demonstrate that the fos protein directly modulates jun function by means of a heterodimer of fos and jun proteins. The fos 'leucine zipper'11 domain is necessary for the DNA binding of the heterodimer; a distinct domain, localized in the C-terminal region of the fos protein, is responsible for transcriptional regulation.

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References

  1. Bohmann, D. et al. Science 238, 1386–1392 (1987).
    Article ADS CAS Google Scholar
  2. Setoyama, C., Frunzio, R., Liau, G., Mudryj, M. & DeCrombrugghe, B. Proc. natn. Acad. Sci. U.S.A. 83, 3213–3217 (1986).
    Article ADS CAS Google Scholar
  3. Distel, R. J., Ro, H.-S., Rosen, B. S., Groves, D. L. & Spiegelman, B. M. Cell 49, 835–844 (1987).
    Article CAS Google Scholar
  4. Rauscher III, F. J., Sambucetti, L. C., Curran, T., Distel, R. J. & Spiegelman, B. M. Cell 52, 471–480 (1988).
    Article CAS Google Scholar
  5. Sassone-Corsi, P., Sisson, J. C. & Verma, I. M. Nature 334, 314–319 (1988).
    Article ADS CAS Google Scholar
  6. Kingston, R. E., Baldwin, A. S. Jr & Sharp, P. A. Nature 312, 280–282 (1984).
    Article ADS CAS Google Scholar
  7. Franza, B. R. Jr, Rauscher III, F. J., Josephs, S. F. & Curran, T. Science 239, 1150–1153 (1988).
    Article ADS CAS Google Scholar
  8. Rauscher III, F. J. et al. Science 240, 1010–1016 (1988).
    Article ADS CAS Google Scholar
  9. Chiu, R. et al. Cell 54, 541–552 (1988).
    Article CAS Google Scholar
  10. Sassone-Corsi, P., Lamph, W. W., Kamps, M. & Verma, I. M. Cell 54, 553–560 (1988).
    Article CAS Google Scholar
  11. Landschulz, W. H., Johnson, P. F. & McKnight, S. L. Science 240, 1759–1764 (1988).
    Article ADS CAS Google Scholar
  12. Sambucetti, L. C. & Curran, T. Science 234, 1417–1419 (1986).
    Article ADS CAS Google Scholar
  13. Renz, M., Neuberg, M., Kurz, C., Bravo, R. & Muller, R. EMBO J 4, 3711–3716 (1985).
    Article CAS Google Scholar
  14. Lamph, W. W., Wamsley, P., Sassone-Corsi, P. & Verma, I. M. Nature 334, 629–631 (1988).
    Article ADS CAS Google Scholar
  15. De Togni, P., Niman, H., Raymond, V., Sawchenko, P. & Verma, I. M. Molec. cell. Biol. 8, 2251–2256 (1988).
    Article CAS Google Scholar
  16. Curran, T., Van Beveren, C., Ling, N. & Verma, I. M. Molec. cell. Biol. 5, 162–172 (1985).
    Article Google Scholar
  17. Angel, P. et al. Cell 49, 729–739 (1988).
    Article Google Scholar
  18. Barber, J. R. & Verma, I. M. Molec. cell. Biol. 7, 2201–2211 (1987).
    Article CAS Google Scholar
  19. Melton, D. et al. Nucleic Acids Res. 12, 7035–7056 (1984).
    Article CAS Google Scholar
  20. Sassone-Corsi, P., Lamph, W. W. & Verma, I. M. Cold Spring Harb. Symp. quant. Biol. 53, (in the press).

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Authors and Affiliations

  1. Molecular Biology and Virology Laboratory, The Salk Institute, PO Box 85800, San Diego, California, 92138, USA
    Paolo Sassone-Corsi, Lynn J. Ransone, William W. Lamph & Inder M. Verma

Authors

  1. Paolo Sassone-Corsi
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  2. Lynn J. Ransone
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  3. William W. Lamph
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  4. Inder M. Verma
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Sassone-Corsi, P., Ransone, L., Lamph, W. et al. Direct interaction between fos and jun nuclear oncoproteins: role of the 'leucine zipper' domain.Nature 336, 692–695 (1988). https://doi.org/10.1038/336692a0

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