Direct interaction between fos and jun nuclear oncoproteins: role of the 'leucine zipper' domain (original) (raw)
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- Published: 15 December 1988
Nature volume 336, pages 692–695 (1988)Cite this article
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Abstract
Gene expression is modulated by the specific interactions of nuclear proteins with unique regulatory sequences in the genome. Proteins involved in transcriptional regulation seem to be either transcription factors or transcription modulators and their interactions are crucial in determining whether the expression of a specific gene is activated or repressed. Recently1, the product of the proto-oncogene jun has been identified as the transcription factor AP-1, whereas nuclear oncoproteins fos and myc have been implicated in transcriptional transregulation of several promoters2–6. Furthermore, the products of the fos and jun proto-oncogenes are associated in some transcription complexes3,5,7. Although the nature of the association is unclear, the two proteins co-immunoprecipitate with fos antibodies in nuclear extracts8–10. Here, we report studies that demonstrate that the fos protein directly modulates jun function by means of a heterodimer of fos and jun proteins. The fos 'leucine zipper'11 domain is necessary for the DNA binding of the heterodimer; a distinct domain, localized in the C-terminal region of the fos protein, is responsible for transcriptional regulation.
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Authors and Affiliations
- Molecular Biology and Virology Laboratory, The Salk Institute, PO Box 85800, San Diego, California, 92138, USA
Paolo Sassone-Corsi, Lynn J. Ransone, William W. Lamph & Inder M. Verma
Authors
- Paolo Sassone-Corsi
You can also search for this author inPubMed Google Scholar - Lynn J. Ransone
You can also search for this author inPubMed Google Scholar - William W. Lamph
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Sassone-Corsi, P., Ransone, L., Lamph, W. et al. Direct interaction between fos and jun nuclear oncoproteins: role of the 'leucine zipper' domain.Nature 336, 692–695 (1988). https://doi.org/10.1038/336692a0
- Received: 05 October 1988
- Accepted: 24 October 1988
- Issue Date: 15 December 1988
- DOI: https://doi.org/10.1038/336692a0