Mapping the transition state and pathway of protein folding by protein engineering (original) (raw)

Nature volume 340, pages 122–126 (1989)Cite this article

Abstract

In the transition state for unfolding of barnase, the hydrophobic core between the major _α_-helix and _β_-sheet is somewhat weakened, the C terminus of the major helix is largely intact but its N terminus is exposed and a major loop has been invaded by solvent.

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References

  1. Creighton, T. E. Proc. natn. Acad. Sci. U.S.A. 85, 5082–5086 (1988).
    Article ADS CAS Google Scholar
  2. Udgaonkar, J. B. & Baldwin, R. L. Nature 335, 694–699 (1988).
    Article ADS CAS Google Scholar
  3. Roder, H., Elöve, G. A. & Englander, S. W. Nature 335, 700–704 (1988).
    Article ADS CAS Google Scholar
  4. Beasty, A. M. et al. Biochemistry 25, 2965–2974 (1989).
    Article Google Scholar
  5. Garvey, E. P. & Matthews, C. R. Biochemistry 28, 2083–2093 (1989).
    Article CAS Google Scholar
  6. Chen, B.-l., Baase, W. A. & Schellman, J. A. Biochemistry 28, 691–699 (1989).
    Article CAS Google Scholar
  7. Goldenberg, D. P., Frieden, R. W., Haack, J. A. & Morrison, T. B. Nature 338, 127–132 (1989).
    Article ADS CAS Google Scholar
  8. Winter, G., Fersht, A. R., Wilkinson, A. J., Zoller, M. & Smith, M. Nature 299, 756–758 (1982).
    Article ADS CAS Google Scholar
  9. Fersht, A. R. Biochemistry 26, 8031–8037 (1987).
    Article CAS Google Scholar
  10. Kellis, J. T. Jr, Nyberg, K., Sali, D. & Fersht, A. R. Nature 333, 784–786 (1988).
    Article ADS CAS Google Scholar
  11. Sali, D., Bycroft, M. & Fersht, A. R. Nature 335, 741–743 (1988).
    ADS Google Scholar
  12. Kellis, J. T. Jr, Nyberg, K. & Fersht, A. R. Biochemistry 28, 4914–4922 (1989).
    Article CAS Google Scholar
  13. Mauguen, Y. et al. Nature 297, 162–164 (1982).
    Article ADS CAS Google Scholar
  14. Schmid, F. X. & Baldwin, R. L. Proc. natn. Acad. Sci. U.S.A. 75, 4764–4768 (1978).
    Article ADS CAS Google Scholar
  15. Fersht, A. R., Leatherbarrow, R. J. & Wells, T. N. C. Biochemistry 26, 6030–6038 (1987).
    Article CAS Google Scholar
  16. Tanford, C. Adv. Protein Chem. 24, 1–95 (1970).
    Article CAS Google Scholar
  17. Fersht, A. R. Enzyme Structure and Mechanism 2nd edn (Freeman, New York, 1985).
    Google Scholar
  18. Fersht, A. R. Biochemistry 27, 1577–1580 (1988).
    Article CAS Google Scholar
  19. Wolfenden, R., Andersson, L., Cullis, P. M. & Southgate, C. C. B. Biochemistry 20, 849–855 (1981).
    Article CAS Google Scholar
  20. Presta, L. G. & Rose, G. D. Science 240, 1632–1641 (1988).
    Article ADS CAS Google Scholar
  21. Richardson, J. S. & Richardson, D. C. Science 240, 1648–1652 (1988).
    Article ADS CAS Google Scholar

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Authors and Affiliations

  1. MRC Unit for Protein Function and Design, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB21EW, UK
    Andreas Matouschek, James T. Kellis Jr, Luis Serrano & Alan R. Fersht

Authors

  1. Andreas Matouschek
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  2. James T. Kellis Jr
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  3. Luis Serrano
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  4. Alan R. Fersht
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Matouschek, A., Kellis, J., Serrano, L. et al. Mapping the transition state and pathway of protein folding by protein engineering.Nature 340, 122–126 (1989). https://doi.org/10.1038/340122a0

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