Triggering of cyclin degradation in interphase extracts of amphibian eggs by cdc2 kinase (original) (raw)

Nature volume 346, pages 379–382 (1990)Cite this article

Abstract

THE cell cycles of early Xenopus embryos consist of a rapid succession of alternating S and M phases1. These cycles are controlled by the activity of a protein kinase complex (cdc2 kinase) which contains two subunits. One subunit is encoded by the frog homologue of the fission yeast cdc2+ gene, p34_cdc2_ (ref. 2) and the other is a cyclin3. The concentration of cyclins follows a sawtooth oscillation because they accumulate in interphase and are destroyed abruptly during mitosis3. The association of cyclin and p34_cdc2_ (refs 4–7) is not sufficient for activation of cdc2 kinase, however; dephosphorylation of key tyrosine and threonine residues of p34_cdc2_ is necessary to turn on its kinase activity8–11. The activity of cdc2 kinase is thus regulated by a combination of translational and post-translational mechanisms. The loss of cdc2 kinase activity at the end of mitosis depends on the destruction of the cyclin subunits3,4,12,13. It has been suggested that this destruction is induced by cdc2 kinase itself, thereby providing a negative feedback loop to terminate mitosis14,15. Here we report direct experimental evidence for this idea by showing that cyclin proteolysis can be triggered by adding cdc2 kinase to a cell-free extract of interphase Xenopus eggs.

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Author notes

  1. Eric Karsenti: To whom correspondence should be addressed

Authors and Affiliations

  1. EMBL, Postfach 102209, 6900, Heidelberg, FRG
    Marie-Anne Félix & Eric Karsenti
  2. CNRS, BP 5051, 34033, Montpellier Cedex, France
    Jean-Claude Labbé & Marcel Dorée
  3. University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge, CB2 1QW, UK
    Tim Hunt

Authors

  1. Marie-Anne Félix
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  2. Jean-Claude Labbé
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  3. Marcel Dorée
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  4. Tim Hunt
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  5. Eric Karsenti
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Félix, MA., Labbé, JC., Dorée, M. et al. Triggering of cyclin degradation in interphase extracts of amphibian eggs by cdc2 kinase.Nature 346, 379–382 (1990). https://doi.org/10.1038/346379a0

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