Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B (original) (raw)

Nature volume 346, pages 760–763 (1990)Cite this article

Abstract

CYCLINS are proteins synthesized during each cell cycle and abruptly destroyed in each mitosis1–5. Cyclins have been implicated in the induction of mitosis6,7 and are associated with the serine–threonine protein kinase p34_cdc2_ as components of mitosis promoting factor (MPF)8–-10. On the basis of conserved sequence motifs cyclins can be divided into A or B types. We recently cloned a human cyclin B (ref. 11) and showed that cyclin B expression is regulated transcriptionally and post-translationally during the cell cycle, and that cyclin B associates with p34_cdc2_. Here we report that human cyclin A messenger RNA and protein levels also vary during the cell cycle, and increase and decrease in advance of cyclin B levels. Cyclin A is associated with a protein of relative molecular mass 33,000 that is related to, but distinct from, p34_cdc2_, and this complex has histone H1 kinase activity in vitro. Cyclin A is identical to p60, a protein that associates with p34_cdc2_ in interphase cells12 and with adenovirus E1 A in transformed cells22.

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References

  1. Evans, T., Rosenthal, E. T., Youngblom, J., Distel, D. & Hunt, T. Cell 33, 389–396 (1983).
    Article CAS Google Scholar
  2. Swenson, K., Farrell, K. M. & Ruderman, J. V. Cell 47, 861–870 (1986).
    Article CAS Google Scholar
  3. Standart, N., Minshull, J., Pines, J. & Hunt, T. Devl Biol. 124, 248–258 (1987).
    Article CAS Google Scholar
  4. Westendorf, J. M., Swenson, K. I. & Ruderman, J. V. J. Cell Biol. 108, 1431–1444 (1989).
    Article CAS Google Scholar
  5. Hunt, T. Curr. Opin. Cell Biol. 1, 286–274 (1989).
    Article Google Scholar
  6. Minshull, J., Blow, J. J. & Hunt, T. Cell 56, 947–956 (1989).
    Article CAS Google Scholar
  7. Murray, A. W. & Kirschner, M. W. Nature 339, 275–280 (1989).
    Article ADS CAS Google Scholar
  8. Draetta, G. et al. Cell 56, 829–838 (1989).
    Article CAS Google Scholar
  9. Labbé, J.-C. et al. EMBO. J. 8, 3053–3058 (1989).
    Article Google Scholar
  10. Gautier, J. et al. Cell 60, 487–494 (1990).
    Article CAS Google Scholar
  11. Pines, J. & Hunter, T. Cell 58, 833–846 (1989).
    Article CAS Google Scholar
  12. Giordano, A. et al. Cell 58, 981–990 (1989).
    Article CAS Google Scholar
  13. Lehner, C. F. & O'Farrell, P. H. Cell 56, 957–968 (1989).
    Article CAS Google Scholar
  14. Hanks, S. K. Proc. natn. Acad. Sci. U.S.A. 84, 388–392 (1987).
    Article ADS CAS Google Scholar
  15. Wang, J., Chenivesse, X., Henglein, B. & Brėchot, C. Nature 343, 555–557 (1990).
    Article ADS CAS Google Scholar
  16. Minshull, J. et al. in Cell Cycle Control in Eukaryotes (eds Beach, D., Basilico, C. & Newport, J.) 128–139 (Cold Spring Harbor Laboratory, New York, 1988).
    Google Scholar
  17. Luca, F. C. & R uderman, J. V. J. Cell Biol. 109, 1895–1909 (1989).
    Article CAS Google Scholar
  18. Gould, K. L. & Nurse, P. Nature 342, 39–45 (1989).
    Article ADS CAS Google Scholar
  19. Pondaven, P., Meijer, L. & Beach, D. Genes Dev. 4, 9–17 (1990).
    Article CAS Google Scholar
  20. Pines, J. & Hunter, T. New Biologist 2, 389–401 (1990).
    CAS PubMed Google Scholar
  21. Richardson, H. E., Wittenberg, C., Cross, F. & Reed, S. I. Cell 59, 1127–1133 (1989).
    Article CAS Google Scholar
  22. Harlow, E., Whyte, P., Franza, B. R.,& Schley, C. Molec. cell. Biol. 6, 1579–1589 (1986).
    Article CAS Google Scholar
  23. Whyte, P. et al. Nature 334, 124–129 (1988).
    Article ADS CAS Google Scholar
  24. De Caprio, J. A. et al. Cell 58, 1085–1095 (1989).
    Article CAS Google Scholar

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  1. Tony Hunter: To whom correspondence should be addressed.

Authors and Affiliations

  1. Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, PO Box 85800, San Diego, California, 92138, USA
    Jonathon Pines & Tony Hunter

Authors

  1. Jonathon Pines
  2. Tony Hunter

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Pines, J., Hunter, T. Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.Nature 346, 760–763 (1990). https://doi.org/10.1038/346760a0

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