Cloning and expression of human and rat Dt dopamine receptors (original) (raw)
- Letter
- Published: 06 September 1990
- David K. Grandy1,
- Lisa Thambi1,3,
- Jake A. Kushner1,
- Hubert H. M. Van Tol1 nAff5,
- Roger Cone1,
- David Pribnow4,
- John Salon1,
- James R. Bunzow1 &
- …
- Olivier Civelli1,4
Nature volume 347, pages 76–80 (1990)Cite this article
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Abstract
THE importance of the dopaminergic system in brain function has been emphasized by its association with neurological and psychiatric disorders such as Parkinson's disease and schizophrenia. On the basis of their biochemical and pharmacological characteristics, dopamine receptors are classified into D1 and D2 subtypes1,2. As the most abundant dopamine receptor in the central nervous system, D1 receptors seem to mediate some behavioural responses3, modulate activity of D2 dopamine receptors4,5, and regulate neuron growth and differentiation6. The D2 dopamine receptor has been cloned by low-stringency screening7. We report here the cloning of human and rat D1 dopamine receptors by applying an approach based on the polymerase chain reaction8. The cloned human D1 dopamine receptor has been characterized on the basis of four criteria: the deduced amino-acid sequence, which reveals that it is a G protein-coupled receptor; the tissue distribution of its messenger RNA, which is compatible with that of the Dl dopamine receptor; its pharmacological profile when transfected into COS-7 cells; and its ability to stimulate the accumulation of cyclic AMP in human 293 cells.
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References
- Kebabian, J. W. & Calne, D. B. Nature 277, 93–96 (1979).
Article ADS CAS PubMed Google Scholar - Creese, I., Sibley, D. R., Hamblin, M. W. & Leff, S. E. A. Rev. Neurosci. 6, 43–71 (1983).
Article CAS Google Scholar - Clark, D. & White, F. J. Synapse 1, P347–P388 (1987).
Article Google Scholar - Walters, J. R., Bergstrom, D. A., Carlson, J. H., Chase, T. N. & Braun, A. R. Science 236, 719–722 (1987).
Article ADS CAS PubMed Google Scholar - Waddington, J. L. J. Psychopharmacol. 3, 54–63 (1989).
Article CAS PubMed Google Scholar - Lankford, K. L., DeMello, F. G. & Klein, W. L. Proc. natn. Acad. Sci. U.S.A. 85, 4567–4571 (1988).
Article ADS CAS Google Scholar - Bunzow, J. R. et al. Nature 336, 783–787 (1988).
Article ADS CAS PubMed Google Scholar - Saiki, R. K. et al. Science 239, 487–491 (1988).
Article ADS CAS PubMed Google Scholar - Dohlman, H. G., Caron, M. G. & Lefkowitz, R. J. Biochemistry 26, 2657–2664 (1987).
Article CAS PubMed Google Scholar - Libert, F. et al. Science 244, 569–572 (1989).
Article ADS CAS PubMed Google Scholar - Boyson, S. J., McGonigle, P. & Molinoff, P. B. J. Neurosci. 6, 3177–3188 (1986).
Article CAS PubMed PubMed Central Google Scholar - O'Dowd, B. F. et al. J. biol. Chem. 263, 15985–15992 (1988).
CAS PubMed Google Scholar - Emorine, L. J. et al. Science 245, 1118–1121 (1989).
Article ADS CAS PubMed Google Scholar - Regan, J. W. et al. Proc. natn. Acad. Sci. U.S.A. 85, 6301–6305 (1988).
Article ADS CAS Google Scholar - Strader, C. D., Candelore, M. R., Hill, W. S., Sigal, I. S. & Dixon, R. A. F. J. biol. Chem. 264, 13572–13578 (1989).
CAS PubMed Google Scholar - Lefkowitz, R. J. et al. Cold Spring Harb. Symp. quant. Biol. 53, 507–514 (1988).
Article CAS PubMed Google Scholar - Jarvie, K. R., Booth, G., Brown, E. M. & Niznik, H. B. Molec. Pharmacol. 36, 566–574 (1989).
CAS Google Scholar - Grandy, D. K. et al. Proc. natn Acad. Sci. U.S.A. 86, 9762–9766 (1989).
Article ADS CAS Google Scholar - Kobilka, B. K. et al. Proc. natn Acad. Sci. U.S.A. 84, 46–50 (1987).
Article ADS CAS Google Scholar - Frielle, T. et al. Proc. natn. Acad. Sci. U.S.A. 84, 7920–7924 (1987).
Article ADS CAS Google Scholar - Cotecchia, S. et al. Proc. natn. Acad. Sci. U.S.A. 85, 7159–7163 (1988).
Article ADS CAS Google Scholar - Kobilka, B. K. et al. Science 238, 650–656 (1987).
Article ADS CAS PubMed Google Scholar - Strader, C. D. et al. J. biol. Chem. 263, 10267–10271 (1988).
CAS PubMed Google Scholar - Venter, J. C., Fraser, C. M., Kerlavage, A. R. & Buck, M. Biochem. Pharmacol. 38, 1197–1208 (1989).
Article CAS PubMed Google Scholar - Kishimoto, A. et al. J. biol. Chem. 260, 12492–12499 (1985).
CAS PubMed Google Scholar - Bouvier, M. et al. Nature 333, 370–374 (1988).
Article ADS CAS PubMed Google Scholar - Cullen, B. R. Meth. Enzym. 152, 684–704 (1987).
Article CAS PubMed Google Scholar - Niznik, H. B. et al. Molec. Pharmacol. 34, 29–36 (1990).
Google Scholar - Chen, C. & Okayama, H. Molec. cell. Biol. 7, 2745–2752 (1987).
Article CAS PubMed PubMed Central Google Scholar - Brown, B. L., Albano, J. D., Ekins, R. P. & Sgherzi, A. M. Biochem. J. 171, 561–562 (1971).
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Author notes
- Hubert H. M. Van Tol
Present address: Department of Pharmacology, Medical Sciences Building, University of Toronto, Toronto, Ontario, M5S 1A8, Canada
Authors and Affiliations
- Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201, USA
Qun-Yong Zhou, David K. Grandy, Lisa Thambi, Jake A. Kushner, Hubert H. M. Van Tol, Roger Cone, John Salon, James R. Bunzow & Olivier Civelli - Department of Biochemistry and Molecular Biology, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201, USA
Qun-Yong Zhou - Department of Pharmacology, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201, USA
Lisa Thambi - Department of Cell Biology and Anatomy, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201, USA
David Pribnow & Olivier Civelli
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Zhou, QY., Grandy, D., Thambi, L. et al. Cloning and expression of human and rat Dt dopamine receptors .Nature 347, 76–80 (1990). https://doi.org/10.1038/347076a0
- Received: 26 April 1990
- Accepted: 15 June 1990
- Issue Date: 06 September 1990
- DOI: https://doi.org/10.1038/347076a0