Cloning and expression of human and rat Dt dopamine receptors (original) (raw)

Nature volume 347, pages 76–80 (1990)Cite this article

Abstract

THE importance of the dopaminergic system in brain function has been emphasized by its association with neurological and psychiatric disorders such as Parkinson's disease and schizophrenia. On the basis of their biochemical and pharmacological characteristics, dopamine receptors are classified into D1 and D2 subtypes1,2. As the most abundant dopamine receptor in the central nervous system, D1 receptors seem to mediate some behavioural responses3, modulate activity of D2 dopamine receptors4,5, and regulate neuron growth and differentiation6. The D2 dopamine receptor has been cloned by low-stringency screening7. We report here the cloning of human and rat D1 dopamine receptors by applying an approach based on the polymerase chain reaction8. The cloned human D1 dopamine receptor has been characterized on the basis of four criteria: the deduced amino-acid sequence, which reveals that it is a G protein-coupled receptor; the tissue distribution of its messenger RNA, which is compatible with that of the Dl dopamine receptor; its pharmacological profile when transfected into COS-7 cells; and its ability to stimulate the accumulation of cyclic AMP in human 293 cells.

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References

  1. Kebabian, J. W. & Calne, D. B. Nature 277, 93–96 (1979).
    Article ADS CAS PubMed Google Scholar
  2. Creese, I., Sibley, D. R., Hamblin, M. W. & Leff, S. E. A. Rev. Neurosci. 6, 43–71 (1983).
    Article CAS Google Scholar
  3. Clark, D. & White, F. J. Synapse 1, P347–P388 (1987).
    Article Google Scholar
  4. Walters, J. R., Bergstrom, D. A., Carlson, J. H., Chase, T. N. & Braun, A. R. Science 236, 719–722 (1987).
    Article ADS CAS PubMed Google Scholar
  5. Waddington, J. L. J. Psychopharmacol. 3, 54–63 (1989).
    Article CAS PubMed Google Scholar
  6. Lankford, K. L., DeMello, F. G. & Klein, W. L. Proc. natn. Acad. Sci. U.S.A. 85, 4567–4571 (1988).
    Article ADS CAS Google Scholar
  7. Bunzow, J. R. et al. Nature 336, 783–787 (1988).
    Article ADS CAS PubMed Google Scholar
  8. Saiki, R. K. et al. Science 239, 487–491 (1988).
    Article ADS CAS PubMed Google Scholar
  9. Dohlman, H. G., Caron, M. G. & Lefkowitz, R. J. Biochemistry 26, 2657–2664 (1987).
    Article CAS PubMed Google Scholar
  10. Libert, F. et al. Science 244, 569–572 (1989).
    Article ADS CAS PubMed Google Scholar
  11. Boyson, S. J., McGonigle, P. & Molinoff, P. B. J. Neurosci. 6, 3177–3188 (1986).
    Article CAS PubMed PubMed Central Google Scholar
  12. O'Dowd, B. F. et al. J. biol. Chem. 263, 15985–15992 (1988).
    CAS PubMed Google Scholar
  13. Emorine, L. J. et al. Science 245, 1118–1121 (1989).
    Article ADS CAS PubMed Google Scholar
  14. Regan, J. W. et al. Proc. natn. Acad. Sci. U.S.A. 85, 6301–6305 (1988).
    Article ADS CAS Google Scholar
  15. Strader, C. D., Candelore, M. R., Hill, W. S., Sigal, I. S. & Dixon, R. A. F. J. biol. Chem. 264, 13572–13578 (1989).
    CAS PubMed Google Scholar
  16. Lefkowitz, R. J. et al. Cold Spring Harb. Symp. quant. Biol. 53, 507–514 (1988).
    Article CAS PubMed Google Scholar
  17. Jarvie, K. R., Booth, G., Brown, E. M. & Niznik, H. B. Molec. Pharmacol. 36, 566–574 (1989).
    CAS Google Scholar
  18. Grandy, D. K. et al. Proc. natn Acad. Sci. U.S.A. 86, 9762–9766 (1989).
    Article ADS CAS Google Scholar
  19. Kobilka, B. K. et al. Proc. natn Acad. Sci. U.S.A. 84, 46–50 (1987).
    Article ADS CAS Google Scholar
  20. Frielle, T. et al. Proc. natn. Acad. Sci. U.S.A. 84, 7920–7924 (1987).
    Article ADS CAS Google Scholar
  21. Cotecchia, S. et al. Proc. natn. Acad. Sci. U.S.A. 85, 7159–7163 (1988).
    Article ADS CAS Google Scholar
  22. Kobilka, B. K. et al. Science 238, 650–656 (1987).
    Article ADS CAS PubMed Google Scholar
  23. Strader, C. D. et al. J. biol. Chem. 263, 10267–10271 (1988).
    CAS PubMed Google Scholar
  24. Venter, J. C., Fraser, C. M., Kerlavage, A. R. & Buck, M. Biochem. Pharmacol. 38, 1197–1208 (1989).
    Article CAS PubMed Google Scholar
  25. Kishimoto, A. et al. J. biol. Chem. 260, 12492–12499 (1985).
    CAS PubMed Google Scholar
  26. Bouvier, M. et al. Nature 333, 370–374 (1988).
    Article ADS CAS PubMed Google Scholar
  27. Cullen, B. R. Meth. Enzym. 152, 684–704 (1987).
    Article CAS PubMed Google Scholar
  28. Niznik, H. B. et al. Molec. Pharmacol. 34, 29–36 (1990).
    Google Scholar
  29. Chen, C. & Okayama, H. Molec. cell. Biol. 7, 2745–2752 (1987).
    Article CAS PubMed PubMed Central Google Scholar
  30. Brown, B. L., Albano, J. D., Ekins, R. P. & Sgherzi, A. M. Biochem. J. 171, 561–562 (1971).
    Article Google Scholar

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Author notes

  1. Hubert H. M. Van Tol
    Present address: Department of Pharmacology, Medical Sciences Building, University of Toronto, Toronto, Ontario, M5S 1A8, Canada

Authors and Affiliations

  1. Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201, USA
    Qun-Yong Zhou, David K. Grandy, Lisa Thambi, Jake A. Kushner, Hubert H. M. Van Tol, Roger Cone, John Salon, James R. Bunzow & Olivier Civelli
  2. Department of Biochemistry and Molecular Biology, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201, USA
    Qun-Yong Zhou
  3. Department of Pharmacology, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201, USA
    Lisa Thambi
  4. Department of Cell Biology and Anatomy, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201, USA
    David Pribnow & Olivier Civelli

Authors

  1. Qun-Yong Zhou
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  2. David K. Grandy
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  3. Lisa Thambi
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  4. Jake A. Kushner
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  5. Hubert H. M. Van Tol
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  6. Roger Cone
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  7. David Pribnow
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  8. John Salon
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  9. James R. Bunzow
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  10. Olivier Civelli
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Zhou, QY., Grandy, D., Thambi, L. et al. Cloning and expression of human and rat Dt dopamine receptors .Nature 347, 76–80 (1990). https://doi.org/10.1038/347076a0

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