'Coatomer': a cytosolic protein complex containing subunits of non-clathrin-coated Golgi transport vesicles (original) (raw)

Nature volume 349, pages 248–251 (1991)Cite this article

Abstract

GOLGI-derived coated vesicles contain a set of coat proteins of relative molecular mass 160,000 (_M_r 160K; α-COP), 110K (β-COP), 98K (γ-COP) and 61K (δ-COP), and several smaller subunits1. We have now identified and purified a cytosolic complex containing the same four coat proteins as those of Golgi transport vesicles. We term this complex the Golgi coat promoter or 'coatomer'. The coatomer also contains polypeptides of _M_r 36K, 35K and 20K. It represents about 0.2% of soluble cytosolic protein. Gel filtration of unfractionated cytosol indicates that β-COP resides exclusively in the coatomer complex. The complex seems to be a likely candidate for the unassembled precursor of Golgi coated vesicles, and its purification should help investigations of the role of coat proteins in membrane budding, for which it is necessary to use a refined cell-free system.

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Author notes

  1. James E. Rothman: Address from 1 June 1991: Program in Molecular Cell Biology, Rockefeller Research Laboratories, Sloan-Kettering Institute, 1275 York Avenue, New York, New York 10021, USA.

Authors and Affiliations

  1. Department of Molecular Biology, Princeton University, Princeton, New Jersey, 08544, USA
    M. Gerard Waters, Tito Serafini & James E. Rothman

Authors

  1. M. Gerard Waters
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  2. Tito Serafini
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  3. James E. Rothman
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Waters, M., Serafini, T. & Rothman, J. 'Coatomer': a cytosolic protein complex containing subunits of non-clathrin-coated Golgi transport vesicles.Nature 349, 248–251 (1991). https://doi.org/10.1038/349248a0

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