Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA (original) (raw)

Nature volume 408, pages 86–89 (2000)Cite this article

Abstract

Two small RNAs regulate the timing of Caenorhabditis elegans development1,2. Transition from the first to the second larval stage fates requires the 22-nucleotide lin-4 RNA1,3,4, and transition from late larval to adult cell fates requires the 21-nucleotide let-7 RNA2. The lin-4 and let-7 RNA genes are not homologous to each other, but are each complementary to sequences in the 3′ untranslated regions of a set of protein-coding target genes that are normally negatively regulated by the RNAs1,2,5,6. Here we have detected let-7 RNAs of ∼21 nucleotides in samples from a wide range of animal species, including vertebrate, ascidian, hemichordate, mollusc, annelid and arthropod, but not in RNAs from several cnidarian and poriferan species, Saccharomyces cerevisiae, Escherichia coli or Arabidopsis. We did not detect lin-4 RNA in these species. We found that let-7 temporal regulation is also conserved: let-7 RNA expression is first detected at late larval stages in C. elegans and Drosophila , at 48 hours after fertilization in zebrafish, and in adult stages of annelids and molluscs. The let-7 regulatory RNA may control late temporal transitions during development across animal phylogeny.

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Acknowledgements

We thank the following people for RNA and tissue samples: T. Heanue, R. Pearse and C. Tabin for chick; J. Gerhart and M. Kirschner for Xenopus and acorn worm; S. Agarwal for Xenopus; N. Stavropoulos for mouse; C. Unabia and K. del Carmen for annelid and mollusc; H. Bode for Hydra; J. Nardone and S. Ferrari for Arabidopsis; P. Sudarsanam for yeast; and D. Selinger for E. coli. The phylogenetic survey in this work was inspired by the NASA Evolution and Development meetings organized by E. Davidson and C. Golden. This work was supported by an NIH grant from NIGMS to G.R. and a grant from the MGH Fund for Medical Discovery to A.E.P.

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Author notes

  1. Amy E. Pasquinelli, Brenda J. Reinhart, Patrick Leahy and Eric Davidson: These authors contributed equally to this work

Authors and Affiliations

  1. Department of Molecular Biology Massachusetts General Hospital, and Department of Genetics, Harvard Medical School, Boston, 02114, Massachusetts, USA
    Amy E. Pasquinelli, Brenda J. Reinhart & Gary Ruvkun
  2. Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, 06520, Connecticut, USA
    Frank Slack & Betsy Maller
  3. Kewalo Marine Lab, Pacific Biomedical Research Center, University of Hawaii, Honolulu, 96813, Hawaii , USA
    Mark Q. Martindale
  4. Howard Hughes Medical Institute, Baylor College of Medicine, Houston, 77030, Texas, USA
    Mitzi I. Kuroda
  5. Research School of Biological Sciences, PO Box 475, Australian National University, Canberra, 2601, ACT, Australia
    David C. Hayward & Eldon E. Ball
  6. Department of Zoology and Entomology University of Queensland, Brisbane, 4072, Queensland, Australia
    Bernard Degnan
  7. Institute of Zoology, University of Basel, Basel, CH-4051, Switzerland
    Peter Müller & Jürg Spring
  8. Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, 02129, USA
    Ashok Srinivasan & Mark Fishman
  9. Department of Biology, Boston University, Boston, 02215, Massachusetts, USA
    John Finnerty
  10. Department of Molecular and Cell Biology, University of California, Berkeley, 94720, California , USA
    Joseph Corbo & Michael Levine
  11. Division of Biology 156-29, California Institute of Technology, Pasadena, 91125, California, USA
    Patrick Leahy & Eric Davidson

Authors

  1. Amy E. Pasquinelli
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  2. Brenda J. Reinhart
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  3. Frank Slack
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  4. Mark Q. Martindale
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  5. Mitzi I. Kuroda
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  6. Betsy Maller
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  7. David C. Hayward
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  8. Eldon E. Ball
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  9. Bernard Degnan
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  10. Peter Müller
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  11. Jürg Spring
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  12. Ashok Srinivasan
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  13. Mark Fishman
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  14. John Finnerty
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  15. Joseph Corbo
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  16. Michael Levine
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  17. Patrick Leahy
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  18. Eric Davidson
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  19. Gary Ruvkun
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Pasquinelli, A., Reinhart, B., Slack, F. et al. Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA.Nature 408, 86–89 (2000). https://doi.org/10.1038/35040556

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