Bacterial dehalorespiration with chlorinated benzenes (original) (raw)

Nature volume 408, pages 580–583 (2000)Cite this article

Abstract

Chlorobenzenes are toxic, highly persistent and ubiquitously distributed environmental contaminants that accumulate in the food chain1. The only known microbial transformation of 1,2,3,5-tetrachlorobenzene (TeCB) and higher chlorinated benzenes is the reductive dechlorination to lower chlorinated benzenes under anaerobic conditions observed with mixed bacterial cultures2,3,4. The lower chlorinated benzenes can subsequently be mineralized by aerobic bacteria. Here we describe the isolation of the oxygen-sensitive strain CBDB1, a pure culture capable of reductive dechlorination of chlorobenzenes. Strain CBDB1 is a highly specialized bacterium that stoichiometrically dechlorinates 1,2,3-trichlorobenzene (TCB), 1,2,4-TCB, 1,2,3,4-TeCB, 1,2,3,5-TeCB and 1,2,4,5-TeCB to dichlorobenzenes or 1,3,5-TCB. The presence of chlorobenzene as an electron acceptor and hydrogen as an electron donor is essential for growth, and indicates that strain CBDB1 meets its energy needs by a dehalorespiratory process. According to their 16S rRNA gene sequences, strain CBDB1, Dehalococcoides ethenogenes5 and several uncultivated bacteria form a new bacterial cluster, of which strain CBDB1 is the first, so far, to thrive on a purely synthetic medium.

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References

  1. Oliver, B. G. & Nicol, K. D. Chlorobenzenes in sediments, water, and selected fish from lakes Superior, Huron, Erie, and Ontario. Environ. Sci. Technol. 16, 532–536 (1982).
    Article ADS CAS Google Scholar
  2. Tiedje, J. M., Boyd, S. A. & Fathepure, B. Z. Anaerobic degradation of chlorinated aromatic hydrocarbons. J. Ind. Microbiol. Suppl. 27, 117–127 (1987).
    CAS Google Scholar
  3. Fathepure, B. Z., Tiedje, J. M. & Boyd, S. A. Reductive dechlorination of hexachlorobenzene to tri- and dichlorobenzenes in anaerobic sewage sludge. Appl. Environ. Microbiol. 54, 327–330 (1988).
    CAS PubMed PubMed Central Google Scholar
  4. Beurskens, J. E. M. et al. Dechlorination of chlorinated benzenes by an anaerobic microbial consortium that selectively mediates the thermodynamic most favorable reactions. Environ. Sci. Technol. 28, 701–706 (1994).
    Article ADS CAS Google Scholar
  5. Maymó-Gatell, X., Chien, Y. T., Gossett, J. M. & Zinder, S. H. Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene. Science 276, 1568–1571 (1997).
    Article Google Scholar
  6. Holliger, C., Wohlfarth, G. & Diekert, G. Reductive dechlorination in the energy metabolism of anaerobic bacteria. FEMS Microbiol. Rev. 22, 383–398 (1999).
    Article Google Scholar
  7. Hugenholtz, P., Pitulle, C., Hershberger, K. L. & Pace, N. R. Novel division level bacterial diversity in a Yellowstone hot spring. J. Bacteriol. 180, 366–376 (1998).
    CAS PubMed PubMed Central Google Scholar
  8. von Wintzingerode, F., Selent, B., Hegemann, W. & Göbel, U. B. Phylogenetic analysis of an anaerobic, trichlorobenzene-transforming microbial consortium. Appl. Environ. Microbiol. 65, 283–286 (1999).
    CAS PubMed PubMed Central Google Scholar
  9. Bosma, T. N. P., van der Meer, J. R., Schraa, G., Tros, M. E. & Zehnder, A. J. B. Reductive dechlorination of all trichloro- and dichlorobenzene isomers. FEMS Microbiol. Ecol. 53, 223–229 (1988).
    Article CAS Google Scholar
  10. Holliger, C., Schraa, G., Stams, A. J. M. & Zehnder, A. J. B. Enrichment and properties of an anaerobic mixed culture reductively dechlorinating 1,2,3-trichlorobenzene to 1,3-dichlorobenzene. Appl. Environ. Microbiol. 58, 1636–1644 (1992).
    CAS PubMed PubMed Central Google Scholar
  11. Middeldorp, P. J. M., de Wolf, J., Zehnder, A. J. B. & Schraa, G. Enrichment and properties of a 1,2,4-trichlorobenzene-dechlorinating methanogenic microbial consortium. Appl. Environ. Microbiol. 63, 1225–1229 (1997).
    CAS PubMed PubMed Central Google Scholar
  12. Adrian, L., Manz, W., Szewzyk, U. & Görisch, H. Physiological characterization of a bacterial consortium reductively dechlorinating 1,2,3- and 1,2,4-trichlorobenzene. Appl. Environ. Microbiol. 64, 496–503 (1998).
    CAS PubMed PubMed Central Google Scholar
  13. Adrian, L., Szewzyk, U. & Görisch, H. Bacterial growth linked to reductive dechlorination of trichlorobenzenes. Biodegradation (in the press).
  14. Giesbrecht, P., Kersten, T., Maidhof, H. & Wecke, J. Staphylococcal cell wall: morphogenesis and fatal variations in the presence of penicillin. Microbiol. Mol. Biol. Rev. 62, 1371–1414 (1998).
    CAS PubMed PubMed Central Google Scholar
  15. Ludwig, W. et al. Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19, 554–568 (1998).
    Article CAS Google Scholar
  16. Pfennig, N. Rhodocyclus purpureus gen. nov. and sp. nov., a ring-shaped, vitamin B12-requiring member of the family Rhodospirillaceae. Int. J. Syst. Bacteriol. 28, 283–288 (1978).
    Article CAS Google Scholar
  17. Zehnder, A. J. B. & Wuhrmann, K. Titanium(III) citrate as a nontoxic oxidation-reduction buffering system for the culture of obligate anaerobes. Science 194, 1165–1166 (1976).
    Article ADS CAS Google Scholar
  18. Kalmbach, S., Manz, W. & Szewzyk, U. Isolation of new bacterial species from drinking water biofilms and proof of their in situ dominance with highly specific 16S rRNA probes. Appl. Environ. Microbiol. 63, 4164–4170 (1997).
    CAS PubMed PubMed Central Google Scholar
  19. Bade, K., Manz, W. & Szewzyk, U. Behavior of sulfate reducing bacteria under oligotrophic conditions and oxygen stress in particle-free systems related to drinking water. FEMS Microbiol. Ecol. 32, 215–233 (2000).
    Article CAS Google Scholar

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Acknowledgements

We thank T. Tran and P. Wendler for technical assistance. This work was supported by the Deutsche Forschungsgemeinschaft.

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Authors and Affiliations

  1. Fachgebiet Technische Biochemie,
    Lorenz Adrian & Helmut Görisch
  2. Fachgebiet Ökologie der Mikroorganismen, Technische Universität, Berlin, 13353, Germany
    Lorenz Adrian & Ulrich Szewzyk
  3. Robert Koch Institut, Berlin, 13353, Germany
    Jörg Wecke

Authors

  1. Lorenz Adrian
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  2. Ulrich Szewzyk
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  3. Jörg Wecke
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  4. Helmut Görisch
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Correspondence toLorenz Adrian.

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Adrian, L., Szewzyk, U., Wecke, J. et al. Bacterial dehalorespiration with chlorinated benzenes.Nature 408, 580–583 (2000). https://doi.org/10.1038/35046063

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