From fixed to FRAP: measuring protein mobility and activity in living cells (original) (raw)
References
Edidin, M., Zagyansky, Y. & Lardner, T. J. Measurement of membrane protein lateral diffusion in single cells. Science191, 466–468 (1976). ArticleCAS Google Scholar
Axelrod, D. et al. Lateral motion of fluorescently labeled acetylcholine receptors in membranes of developing muscle fibers. Proc. Natl Acad. Sci. USA73, 4594–4598 (1976). ArticleCAS Google Scholar
Axelrod, D., Koppel, D. E., Schlessinger, J., Elson, E. & Webb, W. W. Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys. J.16, 1055–1069 (1976). ArticleCAS Google Scholar
Salome, L., Cazeils, J. L., Lopez, A. & Tocanne, J. F. Characterization of membrane domains by FRAP experiments at variable observation areas. Eur. Biophys. J.27, 391–402 (1998). ArticleCAS Google Scholar
Periasamy, N. & Verkman, A. S. Analysis of fluorophore diffusion by continuous distributions of diffusion coefficients: application to photobleaching measurements of multicomponent and anomalous diffusion. Biophys. J.75, 557–567 (1998). ArticleCAS Google Scholar
Arrio-Dupont, M., Foucault, G., Vacher, M., Douhou, A. & Cribier, S. Mobility of creatine phosphokinase and β-enolase in cultured muscle cells. Biophys. J.73, 2667–2673 (1997). ArticleCAS Google Scholar
Periasamy, N., Bicknese, S. & Verkman, A. S. Reversible photobleaching of fluorescein conjugates in air-saturated viscous solutions: singlet and triplet state quenching by tryptophan. Photochem. Photobiol.63, 265–271 (1996). ArticleCAS Google Scholar
Gribbon, P. & Hardingham, T. E. Macromolecular diffusion of biological polymers measured by confocal fluorescence recovery after photobleaching. Biophys. J.75, 1032–1039 (1998). ArticleCAS Google Scholar
Arrio-Dupont, M., Cribier, S., Foucault, G., Devaux, P. F. & d'Albis, A. Diffusion of fluorescently labeled macromolecules in cultured muscle cells. Biophys. J.70, 2327–2332 (1996). ArticleCAS Google Scholar
Reits, E. A. J., Benham, A. M., Plougastel, B., Neefjes, J. & Trowsdale, J. Dynamics of proteasome distribution in living cells. EMBO J.16, 6087–6094 (1997). ArticleCAS Google Scholar
Kao, H. P., Abney, J. R. & Verkman, A. S. Determinants of the translational mobility of a small solute in cell cytoplasm. J. Cell Biol.120, 175–184 (1993). ArticleCAS Google Scholar
Swaminathan, R., Bicknese, S., Periasamy, N. & Verkman, A. S. Cytoplasmic viscosity near the cell plasma membrane: translational diffusion of a small fluorescent solute measured by total internal reflection-fluorescence photobleaching recovery. Biophys. J.71, 1140–1151 (1996). ArticleCAS Google Scholar
Livneh, E. et al. Large deletions in the cytoplasmic kinase domain of the epidermal growth factor receptor do not affect its lateral mobility. J. Cell Biol.103, 327–331 (1986). ArticleCAS Google Scholar
Saffman, P. G. & Delbruck, M. Brownian motion in biological membranes. Proc. Natl Acad. Sci. USA72, 3111–3113 (1975). ArticleCAS Google Scholar
Vaz, W. L., Criado, M., Madeira, V. M., Schoellmann, G. & Jovin, T. M. Size dependence of the translational diffusion of large integral membrane proteins in liquid-crystalline phase lipid bilayers. A study using fluorescence recovery after photobleaching. Biochemistry21, 5608–5612 (1982). ArticleCAS Google Scholar
Kucik, D. F., Elson, E. L. & Sheetz, M. P. Weak dependence of mobility of membrane protein aggregates on aggregate size supports a viscous model of retardation of diffusion. Biophys. J.76, 314–322 (1999). ArticleCAS Google Scholar
Partikian, A., Olveczky, B., Swaminathan, R., Li, Y. & Verkman, A. S. Rapid diffusion of green fluorescent protein in the mitochondrial matrix. J. Cell Biol.140, 821–829 (1998). ArticleCAS Google Scholar
Phair, R. D. & Misteli, T. High mobility of proteins in the mammalian cell nucleus. Nature404, 604–609 (2000). ArticleCAS Google Scholar
Nehls, S. et al. Dynamics and retention of misfolded proteins in native ER membranes. Nature Cell Biol.2, 288–295 (2000). ArticleCAS Google Scholar
Cole, N. B. et al. Diffusional mobility of Golgi proteins in membranes of living cells. Science273, 797–801 (1996). ArticleCAS Google Scholar
Edidin, M., Zuniga, M. C. & Sheetz, M. P. Truncation mutants define and locate cytoplasmic barriers to lateral mobility of membrane glycoproteins. Proc. Natl Acad. Sci. USA91, 3378–3382 (1994). ArticleCAS Google Scholar
Ellenberg, J. et al. Nuclear membrane dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis. J. Cell Biol.138, 1193–1206 (1997). ArticleCAS Google Scholar
Dundr, M., Misteli, T. & Olson, M. O. The dynamics of postmitotic reassembly of the nucleolus. J. Cell Biol.150, 433–446 (2000). ArticleCAS Google Scholar
Marguet, D. et al. Lateral diffusion of GFP-tagged H2Ld molecules and of GFP-TAP1 reports on the assembly and retention of these molecules in the endoplasmic reticulum. Immunity11, 231–240 (1999). ArticleCAS Google Scholar
Vasudevan, C. et al. The distribution and translocation of the G protein ADP-ribosylation factor 1 in live cells is determined by its GTPase activity. J. Cell Sci.111, 1277–1285 (1998). CASPubMed Google Scholar
Yokoe, H. & Meyer, T. Spatial dynamics of GFP-tagged proteins investigated by local fluorescence enhancement. Nature Biotechnol.14, 1252–1256 (1996). ArticleCAS Google Scholar
Reits, E. A., Vos, J. C., Gromme, M. & Neefjes, J. The major substrates for TAP in vivo are derived from newly synthesized proteins. Nature404, 774–778 (2000). ArticleCAS Google Scholar
Dayel, M. J., Hom, E. F. & Verkman, A. S. Diffusion of green fluorescent protein in the aqueous-phase lumen of endoplasmic reticulum. Biophys. J.76, 2843–2851 (1999). ArticleCAS Google Scholar
Alecio, M. R., Golan, D. E., Veatch, W. R. & Rando, R. R. Use of a fluorescent cholesterol derivative to measure lateral mobility of cholesterol in membranes. Proc. Natl Acad. Sci. USA79, 5171–5174 (1982). ArticleCAS Google Scholar