Bcr encodes a GTPase-activating protein for p21rac (original) (raw)

Nature volume 351, pages 400–402 (1991)Cite this article

Abstract

MORE than thirty small guanine nucleotide-binding proteins related to the ras_-encoded oncoprotein, termed Ras or p21ras, are known1. They regulate many fundamental processes in all eukaryotic cells, such as growth, vesicle traffic and cytoskeletal organization. GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, leading to down-regulation of the active GTP-bound form2. For p21_ras two GAP proteins are known, rasGAP and the neurofibromatosis (NF1) gene product2–5. There is evidence that rasGAP may also be a target protein for regulation by Ras and be involved in downstream signalling6–8. We have purified a GAP protein for p21_rho_, which is involved in the regulation of the actin cytoskeleton9. Partial sequencing of rhoGAP reveals significant homology with the product of the bcr(breakpoint cluster region) gene, the translocation breakpoint in Philadelphia chromosome-positive chronic myeloid leukaemias. We show here that the carboxy-terminal domains of the bcr_-encoded protein (Bcr) and of a Bcr-related protein, n-chimaerin, are both GAP proteins for the Ras-related GTP-binding protein, p21_rac. This result suggests that Bcr could be a target for regulation by Rac and has important new implications for the role of bcr translocations in leukaemia.

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  1. Alan Hall: To whom correspondence should be addressed

Authors and Affiliations

  1. Chester Beatty Beatty Laboratories, Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK
    Dagmar Diekmann, Suzanne Brill, Michelle D. Garrett & Alan Hall
  2. Ludwig Institute for Cancer Research, 91 Riding House Street, London, W1P 8BT, UK
    Nicholas Totty & Justin Hsuan
  3. Institute of Neurology, 1 Wakefield Street, London, WC1 1PJ, UK
    Clinton Monfries, Christine Hall & Louis Lim

Authors

  1. Dagmar Diekmann
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  2. Suzanne Brill
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  3. Michelle D. Garrett
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  4. Nicholas Totty
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  5. Justin Hsuan
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  6. Clinton Monfries
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  7. Christine Hall
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  8. Louis Lim
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  9. Alan Hall
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Diekmann, D., Brill, S., Garrett, M. et al. Bcr encodes a GTPase-activating protein for p21_rac_.Nature 351, 400–402 (1991). https://doi.org/10.1038/351400a0

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