Dynamin-like protein encoded by the Drosophila shibire gene associated with vesicular traffic (original) (raw)

Nature volume 351, pages 411–414 (1991)Cite this article

Abstract

TEMPERATURE-sensitive paralysis is the most striking defect of adult Drosophila carrying the shibire mutation1. This is believed to be due to a reversible block of endocytosis, which prevents membrane cycling and thus depletes synaptic vesicles2,3. The shibire mutation also affects many tissues outside the nervous system4–7. We have now mapped and characterized the shibire gene. A 275-kilobase yeast artificial chromosome was subcloned into cosmids, among which the gene was then located by analysing with restriction-fragment length polymorphisms. A 15-kilobase fragment of wild-type DNA rescues the mutant phenotype and the sequence of two mutant alleles show differences with wild type, demonstrating that we have isolated the shibire gene. The gene encodes a protein that is highly similar to rat dynamin8,9, 69% of the amino-acid sequence is identical. Dynamin is a GTP-driven mechanochemical enzyme related to mammalian mx-proteins10 and to the yeast _vps_l gene product11. Because the shibire gene product and dynamin have extensive similarity, we propose that they are cognate homologues. Dynamin causes microtubules to slide along each other _in vitro_12 and in extracts it is associated with a distinct, but so far uncharacterized, membrane fraction13. In light of the shibire phenotype, we suggest that these proteins provide the motor for vesicular transport during endocytosis.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

References

  1. Grigliatti, T. A., Hall, L., Rosenbluth, R. & Suzuki, D. T. Molec. gen. Genet. 120, 107–114 (1973).
    Article CAS Google Scholar
  2. Poodry, C. A. & Edgar, L. J. Cell Biol. 81, 520–527 (1979).
    Article CAS Google Scholar
  3. Koenig, J. H., Kosaka, T. & Ikeda, K. J. Neurosci. 9, 1937–1942 (1989).
    Article CAS Google Scholar
  4. Poodry, C. A., Hall, L. & Suzuki, D. T. Devl Biol. 32, 373–386 (1973).
    Article CAS Google Scholar
  5. Raper Hummon, M. & Costello, W. J. Wilhelm Roux Arch. dev. Biol. 197, 383–393 (1988).
    Article Google Scholar
  6. Kessel, I., Holst, B. D. & Roth, T. F. Proc. natn. Acad. Sci. U.S.A. 86, 4968–4972 (1989).
    Article ADS Google Scholar
  7. Narita, K., Tsuruhara, T., Koenig, J. H. & Ikeda, K. J. cell. Phys. 141, 383–391 (1989).
    Article CAS Google Scholar
  8. Obar, R. A., Collins, C. A., Hammarback, J. A., Shpetner, H. S. & Vallee, R. B. Nature 347, 256–261 (1990).
    Article ADS CAS Google Scholar
  9. Vallee, R. B. & Shpetner, H. S. A. Rev. Biochem. 59, 909–932 (1990).
    Article CAS Google Scholar
  10. Staeheli, P., Haller, O., Boll, W., Lindenman, J. & Wasserman, C. Cell 44, 147–158 (1986).
    Article CAS Google Scholar
  11. Rothman, J. H., Raymond, C. K., Gilbert, T., O'Hara, P. J. & Stevens, T. H. Cell 61, 1063–1074 (1990).
    Article CAS Google Scholar
  12. Shpetner, H. S. & Vallee, R. B. Cell 59, 421–432 (1989).
    Article CAS Google Scholar
  13. Scaife, R. & Margolis, R. L. J. cell Biol. 111, 3023–3033 (1990).
    Article CAS Google Scholar
  14. Garza, D., Ajioka, J. W., Burke, D. T. & Hartl, D. L. Science 246, 641–646 (1989).
    Article ADS CAS Google Scholar
  15. Kim, Y.-T. & Wu, C.-F. J. Neurogenet. 7, 1–14 (1990).
    Article CAS Google Scholar
  16. Kozak, M. Nucleic Acids Res. 15, 8125–8148 (1987).
    Article CAS Google Scholar
  17. Masur, S. K., Kim, Y.-T. & Wu, C.-F. J. Neurogenet. 6, 191–206 (1990).
    Article CAS Google Scholar
  18. Poodry, C. A. Devl Biol. 138, 464–472 (1990).
    Article CAS Google Scholar
  19. Vale, R. D. & Goldstein, L. S. B. Cell 60, 883–885 (1990).
    Article CAS Google Scholar
  20. Hopkins, C. R., Gibson, A., Shipman, M. & Miller, K. Nature 346, 335–339 (1990).
    Article ADS CAS Google Scholar
  21. Pearse, B. M. F. EMBO J. 7, 3331–3336 (1988).
    Article CAS Google Scholar
  22. Arnheiter, H., Skuntz, S., Noteborn, M., Chang, S. & Meier, E. Cell 62, 51–61 (1990).
    Article CAS Google Scholar
  23. Yeh, E., Driscoll, R., Coltrera, M., Olins, A. & Bloom, K. Nature 349, 713–715 (1991).
    Article ADS CAS Google Scholar
  24. Campbell, D. A. Nucleic Acids Res. 17, 458 (1989).
    Article CAS Google Scholar
  25. Pirrotta, V. in Vectors: A Survey of Molecular Cloning Vectors and their Uses (eds Rodriguez, R. L. & Denhardt, D. T.) 437–456 (Butterworths, Boston, 1988).
    Book Google Scholar
  26. Palazzolo, M. J., et al. Gene 88, 25–36 (1990).
    Article CAS Google Scholar

Download references

Author information

Authors and Affiliations

  1. Division of Biology 156-29, California Institute of Technology, Pasadena, California, 91125, USA
    Alexander M. van der Bliek & Elliot M. Meyerowrtz

Authors

  1. Alexander M. van der Bliek
    You can also search for this author inPubMed Google Scholar
  2. Elliot M. Meyerowrtz
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

van der Bliek, A., Meyerowrtz, E. Dynamin-like protein encoded by the Drosophila shibire gene associated with vesicular traffic.Nature 351, 411–414 (1991). https://doi.org/10.1038/351411a0

Download citation