Direct access to serum macromolecules by intraerythrocytic malaria parasites (original) (raw)
- Letter
- Published: 05 September 1991
- R. Spiegel1,
- L. Hsiao1,
- R. J. Howard2,
- R. L. Morris1,
- A. P. Thomas1 &
- …
- T. F. Taraschi1 na1
Nature volume 353, pages 73–75 (1991)Cite this article
Abstract
TRAFFICKING pathways in malaria-infected erythrocytes are complex because the internal parasite is separated from the serum by the erythrocyte and parasitophorous vacuolar membranes1. Intraerythrocytic Plasmodium falciparum parasites can endocytose dextrans, protein A and an IgG2a antibody. Here we show that these macromolecules do not cross the erythrocyte or parasitophorous vacuolar membranes, but rather gain direct access to the aqueous space surrounding the parasite through a parasitophorous duct. Evidence for this structure includes visualization of membranes that are continuous between the parasitophorous vacuolar and erythrocyte membranes, and surface labelling of the parasite with fluorescent macromolecules under conditions that block endocytosis. The parasite can internalize by fluid-phase endocytosis macromolecules from the aqueous compartment surrounding it. Thus, surface antigens on trophozoites and schizonts should be considered as targets for antibody-directed parasiticidal agents.
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Author notes
- T. F. Taraschi: To whom correspondence should be addressed.
Authors and Affiliations
- Department of Pathology and Cell Biology, Jefferson Medical College, 1020 Locust Street, Philadelphia, Pennsylvania, 19107, USA
B. Pouvelle, R. Spiegel, L. Hsiao, R. L. Morris, A. P. Thomas & T. F. Taraschi - DNAX Research Institute, Palo Alto, California, 94304, USA
R. J. Howard
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Pouvelle, B., Spiegel, R., Hsiao, L. et al. Direct access to serum macromolecules by intraerythrocytic malaria parasites.Nature 353, 73–75 (1991). https://doi.org/10.1038/353073a0
- Received: 28 March 1991
- Accepted: 25 July 1991
- Issue Date: 05 September 1991
- DOI: https://doi.org/10.1038/353073a0