Sequence identification of 2,375 human brain genes (original) (raw)

Nature volume 355, pages 632–634 (1992)Cite this article

Abstract

WE recently described a new approach for the rapid characterization of expressed genes by partial DNA sequencing to generate 'expressed sequence tags'1. From a set of 600 human brain complementary DNA clones, 348 were informative nuclear-encoded messenger RNAs. We have now partially sequenced 2,672 new, independent cDNA clones isolated from four human brain cDNA libraries to generate 2,375 expressed sequence tags to nuclear-encoded genes. These sequences, together with 348 brain expressed sequence tags from our previous study, comprise more than 2,500 new human genes and 870,769 base pairs of DNA sequence. These data represent an approximate doubling of the number of human genes identified by DNA sequencing and may represent as many as 5% of the genes in the human genome.

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References

  1. Adams, M. D. et al. Science 252, 1651–1656 (1991).
    Article ADS CAS Google Scholar
  2. Ellisen, L. et al. Cell 66, 649–661 (1991).
    Article CAS Google Scholar
  3. Carthew, R. & Rubin, G. Cell 63, 561–577 (1990).
    Article CAS Google Scholar
  4. Rao, Y., Jan, L. & Jan, Y. Nature 345, 163–167 (1990).
    Article ADS CAS Google Scholar
  5. Woods, D. & Bryant, P. Cell 66, 451–464 (1991).
    Article CAS Google Scholar
  6. Finkelstein, R., Smouse, D. Capaci, T., Spradling, A. & Perrimon, N. Genes Dev. 4, 1516–1527 (1990).
    Article CAS Google Scholar
  7. Uberbacher, E. & Mural, R. Proc. natn. Acad. Sci. U.S.A. 88, 11261–11265 (1991).
    Article ADS CAS Google Scholar
  8. Kamholtz, J., de Ferra, F., Puckett, C. & Lazzarini, R. Proc. natn. Acad. Sci. U.S.A. 83, 4962–4966 (1986).
    Article ADS Google Scholar
  9. Galau, G., Klein, W., Britten, R. & Davidson, E. Archs Biochem. Biophys. 197, 584–599 (1977).
    Article Google Scholar
  10. Casero, R. et al. J. biol. Chem. 266, 810–814 (1991).
    CAS PubMed Google Scholar
  11. Young, M. et al. Genomics 7, 491–502 (1990).
    Article CAS Google Scholar
  12. Höög, C. Nucleic Acids Res. 19, 6123–6127 (1991).
    Article Google Scholar
  13. McKusick, V. FASEB J. 5, 12–20 (1991).
    Article CAS Google Scholar
  14. Rubenstein, J. et al. Nucleic Acids Res. 18, 4833–4842.
  15. Schmid, C. W. & Jalinek, W. R. Science 216, 1065–1070 (1982).
    Article ADS CAS Google Scholar
  16. Paulson, K. E. et al. Nature 316, 359–361 (1985).
    Article ADS CAS Google Scholar
  17. Fanning, T. G. & Singer, M. F. Biochim biophys. Acta 910, 203–212 (1987).
    Article CAS Google Scholar
  18. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. molec. Biol. 215, 403–410 (1990).
    Article CAS Google Scholar
  19. Barker, W., George, D., Hunt, L. & Garavelli, J. Nucleic Acids Res. 19 (Suppl), 2231–2236 (1991).
    Article CAS Google Scholar
  20. Pearson, P. Nucleic Acids Res. 19 (Suppl), 2237–2239 (1991).
    Article CAS Google Scholar

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Authors and Affiliations

  1. Receptor Biochemistry and Molecular Biology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892, USA
    Mark D. Adams, Mark Dubnick, Anthony R. Kerlavage, Ruben Moreno, Jenny M. Kelley, Teresa R. Utterback, James W. Nagle, Chris Fields & J. Craig Venter

Authors

  1. Mark D. Adams
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  2. Mark Dubnick
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  3. Anthony R. Kerlavage
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  4. Ruben Moreno
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  5. Jenny M. Kelley
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  6. Teresa R. Utterback
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  7. James W. Nagle
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  8. Chris Fields
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  9. J. Craig Venter
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Adams, M., Dubnick, M., Kerlavage, A. et al. Sequence identification of 2,375 human brain genes.Nature 355, 632–634 (1992). https://doi.org/10.1038/355632a0

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