Prevention of HIV-1 infection in chimpanzees by gpl20 V3 domain-specific monoclonal antibody (original) (raw)

• Letter
• Published: 20 February 1992
• W. A. Schleif1,
• J. H. Nunberg1 nAff6,
• A. J. Conley1,
• Y. Eda2,
• S. Tokiyoshi2,
• S. D. Putney3 nAff7,
• S. Matsushrta4,
• K. E. Cobb5,
• C. M. Jett5,
• J. W. Eichberg5 nAff8 &
• …
• K. K. Murthy5

Nature volume 355, pages 728–730 (1992)Cite this article

• 305 Accesses
• 458 Citations
• 13 Altmetric
• Metrics details

Abstract

THE acquired immunodeficiency syndrome (AIDS) is the late-stage clinical manifestation of long-term persistent infection with the human immunodeficiency virus type 1 (HIV-1). Immune responses directed against the virus and against virus-infected cells during the persistent infection fail to mediate resolution of the infection. As a result, a successful AIDS vaccine must elicit an immune state that will prevent the establishment of the persistent infection following introduction of the virus into the host. The third hyper-variable (V3) domain of the HIV-1 gp120 envelope glycoprotein is a disulphide-linked closed loop of about 30 amino acids which binds and elicits anti-HIV-1 type-specific virus-neutralizing antibodies1–7. The in vitro characteristics of anti-V3 domain antibody suggest that this antibody could by itself prevent HIV-1 infection _in vivo_8,9, an idea supported by chimpanzee challenge studies in which protection against the HIV-1 persistent infection seemed to correlate with the presence of anti-V3 domain antibody10–12. Here we directly demonstrate the protective efficacy of anti-V3 domain antibody in vivo and propose that this antibody is potentially useful as both a pre- and post-exposure prophylactic agent.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

• Purchase on SpringerLink
• Instant access to full article PDF

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

• Log in
• Learn about institutional subscriptions
• Read our FAQs
• Contact customer support

Similar content being viewed by others

References

1. Leonard, C. K. et al J. biol. Chem. 265, 10373–10383 (1990).
   CAS PubMed Google Scholar
2. Goudsmit, J. et al. AIDS 2, 157–164 (1988).
   Article CAS Google Scholar
3. Goudsmit, J. et al. Proc. natn. Acad. Sci. U.S.A. 85, 4478–4482 (1988).
   Article ADS CAS Google Scholar
4. Ho, D. D. et al. J. Virol 61, 2024–2028 (1987).
   CAS PubMed PubMed Central Google Scholar
5. Javaherlan, K. et al. Proc. natn. Acad. Sci. U.S.A. 86, 6768–6772 (1989).
   Article ADS Google Scholar
6. Kenealy, W. R. et al. AIDS Res. Human Retroviruses 5, 173–182 (1989).
   Article CAS Google Scholar
7. Rusche, J. R. et al. Proc. natn. Acad. Sci. U.S.A. 85, 3198–3202 (1988).
   Article ADS CAS Google Scholar
8. Linsley, P. S., Ledbetter, J. A., Kinney-Thomas, E. & Hu, S.-L. J. Virol. 62, 3695–3702 (1988).
   CAS PubMed PubMed Central Google Scholar
9. Skinner, M. A. et al. J. Virol. 62, 4195–4200 (1988).
   CAS PubMed PubMed Central Google Scholar
10. Berman, P. W. et al. Nature 345, 622–625 (1990).
    Article ADS CAS Google Scholar
11. Girard, M. et al. Proc. natn. Acad. Sci. U.S.A. 88, 542–546 (1991).
    Article ADS CAS Google Scholar
12. Emini, E. A. et al. J. Virol. 64, 3674–3678 (1990).
    CAS PubMed PubMed Central Google Scholar
13. Matsushita, S. et al. J. Virol. 62, 2107–2114 (1988).
    CAS PubMed PubMed Central Google Scholar
14. Robertson, G. A., Kostek, B. M., Schleif, W. A., Lewis, J. A. & Emini, E. A. J. virol. Meth. 20, 195–202 (1988).
    Article CAS Google Scholar
15. Javaherian, K. et al. Science 250, 1590–1593 (1990).
    Article ADS CAS Google Scholar

Download references

Author information

Author notes

1. J. H. Nunberg
   Present address: Genentech Inc., South San Francisco, California, 94080, USA
2. S. D. Putney
   Present address: Alkermes Inc., Cambridge, Massachusetts, 02139, USA
3. J. W. Eichberg
   Present address: Wyeth-Ayerst Research, Philadelphia, Pennsyl-vania, 19087, USA

Authors and Affiliations

1. Merck, Sharp and Dohme Research Laboratories, West Point, Pennsylvania, 19486, USA
   E. A. Emini, W. A. Schleif, J. H. Nunberg & A. J. Conley
2. The Chemo-Sero-Therapeutic Research Institute, Kumamoto, 861-15, Japan
   Y. Eda & S. Tokiyoshi
3. Repligen Corporation, Cambridge, Massachusetts, 02139, USA
   S. D. Putney
4. Kumamoto University, Kumamoto, 860, Japan
   S. Matsushrta
5. Southwest Foundation for Biomedical Research, San Antonio, Texas, 78228, USA
   K. E. Cobb, C. M. Jett, J. W. Eichberg & K. K. Murthy

Authors

1. E. A. Emini
2. W. A. Schleif
3. J. H. Nunberg
4. A. J. Conley
5. Y. Eda
6. S. Tokiyoshi
7. S. D. Putney
8. S. Matsushrta
9. K. E. Cobb
10. C. M. Jett
11. J. W. Eichberg
12. K. K. Murthy

Rights and permissions

About this article

Cite this article

Emini, E., Schleif, W., Nunberg, J. et al. Prevention of HIV-1 infection in chimpanzees by gpl20 V3 domain-specific monoclonal antibody.Nature 355, 728–730 (1992). https://doi.org/10.1038/355728a0

Download citation

• Received: 08 October 1991
• Accepted: 21 November 1991
• Issue Date: 20 February 1992
• DOI: https://doi.org/10.1038/355728a0

This article is cited by