Aminopeptidase N is a major receptor for the enteropathogenic coronavirus TGEV (original) (raw)

Nature volume 357, pages 417–420 (1992)Cite this article

Abstract

CORONAVIRUSES, like many animal viruses, are characterized by a restricted host range and tissue tropism1. Transmissible gastroenteritis virus (TGEV), a major pathogen causing a fatal diarrhoea in newborn pig, replicates selectively in the differentiated enterocytes covering the villi of the small intestine2. To investigate the molecular determinants of the infection, we characterized the surface molecule used by the virus for binding and entry into host cells. Here we report that aminopeptidase N, an ectoenzyme abundantly expressed at the apical membrane of the enterocytes, serves as a receptor for TGEV. Monoclonal antibodies were selected for their ability to block infection by TGEV of porcine cell lines. They recognized a brush-border membrane protein of M r, 150K, which was identified as aminopeptidase N by ammo-terminal sequencing. Two lines of evidence supported the view that the peptidase itself acts as a receptor. First, virions bound specifically to aminopep-tidase N that was purified to homogeneity. Second, recombinant expression of aminopeptidase N conferred infectivity by TGEV to an otherwise non-permissive cell line.

Similar content being viewed by others

Article PDF

References

  1. Siddell, S., Wege, H. & Ter Meulen, V. J. gen. Virol. 64, 761–776 (1983).
    Article Google Scholar
  2. Pensaert, M., Haelterman, E. O. & Burstein, T. Arch. Gesamte. Virusforch. 31, 321–334 (1970).
    Article CAS Google Scholar
  3. Olsen, J., Sjöström, H. & Norén, O. FEBS Lett. 251, 275–281 (1989).
    Article CAS Google Scholar
  4. Gorvel, J. P. et al. J. Cell Biol. 108, 2193–2200 (1989).
    Article CAS Google Scholar
  5. Sjöström, H. et al. Eur. J. Biochem. 88, 503–511 (1978).
    Article Google Scholar
  6. Kramers, M. T. C. & Robinson, G. B. Eur. J. Biochem. 99, 345–351 (1979).
    Article CAS Google Scholar
  7. Maddon, P. J. et al. Cell 47, 333–348 (1986).
    Article CAS Google Scholar
  8. Greve, J. M. et al. Cell, 56, 839–847 (1989).
    Article CAS Google Scholar
  9. Mendelsohn, C. L., Wimmer, E. & Racaniello, V. R. Cell, 56, 855–865 (1989).
    Article CAS Google Scholar
  10. Dveksler, G. S. et al. J. Virol. 65, 6881–6891 (1991).
    CAS PubMed PubMed Central Google Scholar
  11. Kim, J. W., Closs, E. I., Albritton, L. M. & Cunningham, J. M. Nature 352, 725–728 (1991).
    Article ADS CAS Google Scholar
  12. Maroux, S., Louvard, D. & Baratti, J. Biochim. biophys. Acta 321, 282–295 (1973).
    Article CAS Google Scholar
  13. Olsen, J. et al. FEBS Lett. 238, 307–314 (1988).
    Article CAS Google Scholar

Download references

Author information

Author notes

  1. Hubert Laude: To whom correspondence should be addressed.

Authors and Affiliations

  1. Unité de Virologie et Immunologie Moléculaires, INRA, Domaine de Vilvert, 78350, Jouy-en-josas, France
    Bernard Delmas, Jacqueline Gelfi, René L'Haridon & Hubert Laude
  2. Department of Biochemistry C, Panum Institute, DK 2200, Copenhagen N, Denmark
    Vogel, Hans Sjöström & Norén

Authors

  1. Bernard Delmas
    You can also search for this author inPubMed Google Scholar
  2. Jacqueline Gelfi
    You can also search for this author inPubMed Google Scholar
  3. René L'Haridon
    You can also search for this author inPubMed Google Scholar
  4. Vogel
    You can also search for this author inPubMed Google Scholar
  5. Hans Sjöström
    You can also search for this author inPubMed Google Scholar
  6. Norén
    You can also search for this author inPubMed Google Scholar
  7. Hubert Laude
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Delmas, B., Gelfi, J., L'Haridon, R. et al. Aminopeptidase N is a major receptor for the enteropathogenic coronavirus TGEV.Nature 357, 417–420 (1992). https://doi.org/10.1038/357417a0

Download citation

This article is cited by