Aminopeptidase N is a major receptor for the enteropathogenic coronavirus TGEV (original) (raw)
- Letter
- Published: 04 June 1992
- Jacqueline Gelfi1,
- René L'Haridon1,
- Vogel2,
- Hans Sjöström2,
- Norén2 &
- …
- Hubert Laude1 na1
Nature volume 357, pages 417–420 (1992)Cite this article
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Abstract
CORONAVIRUSES, like many animal viruses, are characterized by a restricted host range and tissue tropism1. Transmissible gastroenteritis virus (TGEV), a major pathogen causing a fatal diarrhoea in newborn pig, replicates selectively in the differentiated enterocytes covering the villi of the small intestine2. To investigate the molecular determinants of the infection, we characterized the surface molecule used by the virus for binding and entry into host cells. Here we report that aminopeptidase N, an ectoenzyme abundantly expressed at the apical membrane of the enterocytes, serves as a receptor for TGEV. Monoclonal antibodies were selected for their ability to block infection by TGEV of porcine cell lines. They recognized a brush-border membrane protein of M r, 150K, which was identified as aminopeptidase N by ammo-terminal sequencing. Two lines of evidence supported the view that the peptidase itself acts as a receptor. First, virions bound specifically to aminopep-tidase N that was purified to homogeneity. Second, recombinant expression of aminopeptidase N conferred infectivity by TGEV to an otherwise non-permissive cell line.
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- Hubert Laude: To whom correspondence should be addressed.
Authors and Affiliations
- Unité de Virologie et Immunologie Moléculaires, INRA, Domaine de Vilvert, 78350, Jouy-en-josas, France
Bernard Delmas, Jacqueline Gelfi, René L'Haridon & Hubert Laude - Department of Biochemistry C, Panum Institute, DK 2200, Copenhagen N, Denmark
Vogel, Hans Sjöström & Norén
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- Bernard Delmas
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Delmas, B., Gelfi, J., L'Haridon, R. et al. Aminopeptidase N is a major receptor for the enteropathogenic coronavirus TGEV.Nature 357, 417–420 (1992). https://doi.org/10.1038/357417a0
- Received: 05 February 1992
- Accepted: 02 April 1992
- Issue Date: 04 June 1992
- DOI: https://doi.org/10.1038/357417a0