T-complex polypeptide-1 is a subunit of a heteromeric particle in the eukaryotic cytosol (original) (raw)

Nature volume 358, pages 249–252 (1992)Cite this article

Abstract

THE murine t-complex encodes t-complex polypeptide-1 (TCP1)1, which is constitutively expressed in almost all cells, and upregulated during spermatogenesis2,3. Mammalian sequences have >96% identity with each other, and >60% identity with Drosophila melanogaster and yeast orthologues4–9. TCP1 is essential in yeast9, and is postulated to be the cytosolic mammalian equivalent of groEL10,11. We report here that, in the native state, murine and human TCP1 is distributed throughout the cytosol as an 800K–950K hetero-oligomeric particle in association with four to six unidentified proteins and two Hsp70 heat-shock proteins. Negative-stain electron microscopy indicates that the structure is two stacked rings, 12–16 nm in diameter. Therefore, despite similarities with the chaperonin 60 proteins, these data indicate that TCP1 is biochemically and structurally unique. We suggest that TCP1 may represent one of a family of molecules in the eukaryotic cytosol involved in protein folding and regulated in part by their heteromeric associations.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

References

  1. Silver, L. M., Artzt, K. & Bennett, D. Cell 17, 275–284 (1979).
    Article CAS Google Scholar
  2. Willison, K. et al. Cell 57, 621–632 (1989).
    Article CAS Google Scholar
  3. Willison, K. R., Hynes, G., Davies, P., Goldsborough, A. & Lewis, V. A. Genet. Res. 56, 193–201 (1990).
    Article CAS Google Scholar
  4. Kirchhoff, C. & Willison, K. Nucleic Acids Res. 18, 4248 (1990).
    Article Google Scholar
  5. Willison, K. R., Dudley, K. & Potter, J. Cell 44, 727–738 (1986).
    Article CAS Google Scholar
  6. Ahmad, S. & Gupta, R. S. Biochim. biophys. Acta 1089, 253–255 (1990).
    Article Google Scholar
  7. Morita, T., Kubota, H., Gachelin, G., Nozaki, M. & Matsushiro, A. Biochim. biophys. Acta 1129, 96–99 (1991).
    Article CAS Google Scholar
  8. Ursic, D. & Ganetsky, B. Gene 68, 267–274 (1988).
    Article CAS Google Scholar
  9. Ursic, D. & Culbertson, M. R. Molec. cell. Biol. 11, 2629–2640 (1991).
    CAS PubMed PubMed Central Google Scholar
  10. Gupta, R. S. Biochem. Int. 4, 833–839 (1990).
    Google Scholar
  11. Ellis, R. J. Science 250, 954–959 (1990).
    Article ADS CAS Google Scholar
  12. Linquist, S. & Craig, E. A. Rev. Genet. 22, 631–677 (1988).
    Article Google Scholar
  13. Maekawa, M. O'Brien, D. A., Allen, R. L. & Eddy, E. M. Biol. Repro. 40, 843–852 (1989).
    Article CAS Google Scholar
  14. Hendrix, R. W. J. molec. Biol. 129, 375–392 (1979).
    Article CAS Google Scholar
  15. Hutchinson, E. G. et al. EMB0 J. 8, 1485–1490 (1989).
    Article CAS Google Scholar
  16. Phipps, B. M., Hoffmann, A., Stetter, K. O. & Baumeister, W. EMBO J. 10, 1711–1722 (1991).
    Article CAS Google Scholar
  17. Trent, J. D., Nimmesgern, E., Wall, J. S., Hartl, F.-U. & Horwich, A. L. Nature 354, 490–493 (1991).
    Article ADS CAS Google Scholar
  18. Dahlmann, B. et al. FEBS Lett. 251, 125–131 (1989).
    Article CAS Google Scholar
  19. Hanks, S. K., Quinn, A. M. & Hunter, T. Science 241, 42–52 (1988).
    Article ADS CAS Google Scholar
  20. Ellis, R. J. & van der Vies, S. M. A. Rev. Biochem. 60, 321–347 (1991).
    Article CAS Google Scholar
  21. Yaffe, M. B. et al. Nature 358, 245–248 (1992).
    Article ADS CAS Google Scholar
  22. Kim, Y. S., Perdomo, J. & Nordberg, J. J. biol. Chem. 246, 5466–5476 (1971).
    Article CAS Google Scholar
  23. Corbett, J. & Dunn, M. J. Methods in Molecular Biology: Membrane Methods (eds Graham, J. & Higgins, J.) (Humana, New Jersey, in the press).
  24. Mehra, V., Sweetser, D. & Young, R. A. Proc. natn. Acad. Sci. U.S.A. 83, 7013–7017 (1986).
    Article ADS CAS Google Scholar
  25. Hemmingsen, S. M. et al. Nature 333, 330–334 (1988).
    Article ADS CAS Google Scholar
  26. Waterston, R. et al. Nature Genet. 1, 114–123 (1992).
    Article CAS Google Scholar

Download references

Author information

Authors and Affiliations

  1. Institute of Cancer Research, Chester Beatty Laboratories, Fulham Road, London, SW3 6JB, UK
    Victoria A. Lewis, Gillian M. Hynes, Dong Zheng, Helen Saibil & Keith Willison
  2. Department of Crystallography, Birkbeck College, London, WC1E 7HX, UK
    Dong Zheng & Helen Saibil

Authors

  1. Victoria A. Lewis
    You can also search for this author inPubMed Google Scholar
  2. Gillian M. Hynes
    You can also search for this author inPubMed Google Scholar
  3. Dong Zheng
    You can also search for this author inPubMed Google Scholar
  4. Helen Saibil
    You can also search for this author inPubMed Google Scholar
  5. Keith Willison
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Lewis, V., Hynes, G., Zheng, D. et al. T-complex polypeptide-1 is a subunit of a heteromeric particle in the eukaryotic cytosol.Nature 358, 249–252 (1992). https://doi.org/10.1038/358249a0

Download citation

This article is cited by