Continuum of overlapping clones spanning the entire human chromosome 21q (original) (raw)

• Article
• Published: 01 October 1992
• Philippe Rigault2,
• Sophie Guillou2,
• Pierre Ougen1,
• Alain Billaut1,
• Ghislaine Guasconi2,
• Patricia Gervy2,
• Isabelle LeGall1,
• Pascal Soularue1,
• Laurent Grinas2,
• Lydie Bougueleret1,
• Christine Bellanné-Chantelot1,
• Bruno Lacroix1,
• Emmanuel Barillot2,
• Philippe Gesnouin2,
• Stuart Pook2,
• Guy Vaysseix1,2,
• Gerard Frelat3,
• Annette Schmitz3,
• Jean-Luc Sambucy1,
• Assumpcio Bosch4,
• Xavier Estivill4,
• Jean Weissenbach2,5,
• Alain Vignal2,
• Harold Riethman6,
• David Cox7,
• David Patterson8,
• Kathleen Gardiner8,
• Masahira Hattori9,
• Yoshiyuki Sakaki9,
• Hitoshi Ichikawa10,
• Misao Ohki10,
• Denis Le Paslier1,
• Roland Heilig11,
• Stylianos Antonarakis12 &
• …
• Daniel Cohen1,2

Nature volume 359, pages 380–387 (1992)Cite this article

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Abstract

A continuous array of overlapping clones covering the entire human chromosome 21q was constructed from human yeast artificial chromosome libraries using sequence-tagged sites as landmarks specifically detected by polymerase chain reaction. The yeast artificial chromosome contiguous unit starts with pericentromeric and ends with subtelomeric loci of 21q. The resulting order of sequence-tagged sites is consistent with other physical and genetic mapping data. This set of overlapping clones will promote our knowledge of the structure of this chromosome and the function of its genes.

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Authors and Affiliations

1. Centre d'Etude du Polymorphisme Humain (CEPH), 27 rue Juliette Dodu, 75010, Paris, France
   Ilya Chumakov, Pierre Ougen, Alain Billaut, Isabelle LeGall, Pascal Soularue, Lydie Bougueleret, Christine Bellanné-Chantelot, Bruno Lacroix, Guy Vaysseix, Jean-Luc Sambucy, Denis Le Paslier & Daniel Cohen
2. Genethon, 1 rue de I'lnternationale, BP 59, 91002, Evry Cedex, France
   Ilya Chumakov, Philippe Rigault, Sophie Guillou, Ghislaine Guasconi, Patricia Gervy, Laurent Grinas, Emmanuel Barillot, Philippe Gesnouin, Stuart Pook, Guy Vaysseix, Jean Weissenbach, Alain Vignal & Daniel Cohen
3. Centre d'Energie Atomique, 68 Avenue Division LeClerc, 92260, Fontenay aux Roses, France
   Gerard Frelat & Annette Schmitz
4. Institut de Recerca Oncologica, Hospital Duran i Reynals Ctra Castelldefels, KM 2.7, Barcelona, 08907, Spain
   Assumpcio Bosch & Xavier Estivill
5. CNRS URA 1445, Institut Pasteur, 28 rue Docteur Roux, 75724, Paris, France
   Jean Weissenbach
6. The Wistar Institute, 36th Street and Spruce, Philadelphia, Pennsylvania, 19104, USA
   Harold Riethman
7. Neurogenetics Laboratory, University of California, 401 Parnassus Avenue, San Francisco, California, 94143, USA
   David Cox
8. Eleanor Roosevelt Institute, 1899 Gaylord Street, Denver, Colorado, 80206, USA
   David Patterson & Kathleen Gardiner
9. The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minatoku, Tokyo, 108, Japan
   Masahira Hattori & Yoshiyuki Sakaki
10. Saitama Cancer Center Research Institute, Ina-machi, Kitaadachi-gun, Saitama-ken, 362, Japan
    Hitoshi Ichikawa & Misao Ohki
11. INSERM U184, Laboratoire de Génétique Moléculaire de Eucaryotes, 11 rue Humann, Strasbourg, France
    Roland Heilig
12. Center for Medical Genetics, The Johns Hopkins University School of Medicine, 600 North Wolfe Street CMSC 1003, Baltimore, Maryland, 21205, USA
    Stylianos Antonarakis

Authors

1. Ilya Chumakov
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2. Philippe Rigault
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3. Sophie Guillou
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4. Pierre Ougen
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5. Alain Billaut
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6. Ghislaine Guasconi
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7. Patricia Gervy
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8. Isabelle LeGall
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9. Pascal Soularue
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10. Laurent Grinas
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11. Lydie Bougueleret
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12. Christine Bellanné-Chantelot
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13. Bruno Lacroix
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14. Emmanuel Barillot
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15. Philippe Gesnouin
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16. Stuart Pook
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17. Guy Vaysseix
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18. Gerard Frelat
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19. Annette Schmitz
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20. Jean-Luc Sambucy
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21. Assumpcio Bosch
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22. Xavier Estivill
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23. Jean Weissenbach
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24. Alain Vignal
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25. Harold Riethman
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26. David Cox
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27. David Patterson
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28. Kathleen Gardiner
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29. Masahira Hattori
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30. Yoshiyuki Sakaki
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31. Hitoshi Ichikawa
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32. Misao Ohki
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33. Denis Le Paslier
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34. Roland Heilig
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35. Stylianos Antonarakis
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36. Daniel Cohen
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Chumakov, I., Rigault, P., Guillou, S. et al. Continuum of overlapping clones spanning the entire human chromosome 21q.Nature 359, 380–387 (1992). https://doi.org/10.1038/359380a0

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• Received: 01 September 1992
• Accepted: 04 September 1992
• Issue Date: 01 October 1992
• DOI: https://doi.org/10.1038/359380a0

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