Signal-sequence recognition by an Escherichia coli ribonucleoprotein complex (original) (raw)
- Letter
- Published: 22 October 1992
- Stephen High1,
- Heather Wood1,
- Angelika Giner1,
- David Tollervey1 &
- …
- Bernhard Dobberstein1
Nature volume 359, pages 741–743 (1992)Cite this article
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Abstract
HYDROPHOBIC signal-sequences direct the transfer of secretory proteins across the inner membrane of prokaryotes and the endoplasmic reticulum membranes of eukaryotes1. In mammalian cells, signal-sequences are recognized by the 54K protein (_M_r 54,000) of the signal recognition particle (SRP)2,3 which is believed to hold the nascent chain in a translocation-competent conformation until it contacts the endoplasmic reticulum membrane4. The SRP consists of a 7S RNA and six different polypeptides. The 7S RNA and the 54K signal-sequence-binding protein (SRP54) of mammalian SRP exhibit strong sequence similarity to the 4.5S RNA and P48 protein (Ff h) of _Escherichia coli_5–7 which form a ribonucleoprotein particle. Depletion of 4.5S RNA or overproduction of P48 causes the accumulation of the β-lactamase precursor, although not of other secretory proteins8,9. Whether 4.5S RNA and P48 are part of an SRP-like complex with a role in protein export is controversial. Here we show that the P48/4.5S RNA ribonucleoprotein complex interacts specifically with the signal sequence of a nascent secretory protein and therefore is a signal recognition particle.
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References
- von Heijne, G. Biochim. biophys. Acta 947, 307–333 (1988).
Article CAS Google Scholar - Krieg, U. C., Walter, P. & Johnson, A. E. Proc. natn. Acad. Sci. U.S.A. 83, 8604–8608 (1986).
Article ADS CAS Google Scholar - Kurzchalia, T. V. et al. Nature 320, 634–636 (1986).
Article ADS CAS Google Scholar - Rapoport, T. A. Trends biochem. Sci. 15, 355–358 (1990).
Article CAS Google Scholar - Poritz, M. A., Strub, K. & Walter, P. Cell 55, 4–6 (1988).
Article CAS Google Scholar - Römisch, K. et al. Nature 340, 478–482 (1989).
Article ADS Google Scholar - Bernstein, H. D. et al. Nature 340, 482–486 (1989).
Article ADS CAS Google Scholar - Poritz, M. A. et al. Science 250, 1111–1117 (1990).
Article ADS CAS Google Scholar - Ribes, V., Römisch, K., Giner, A., Dobberstein, B. & Tollervey, D. Cell 63, 591–600 (1990).
Article CAS Google Scholar - Brown, S. New Biol. 3, 430–438 (1991).
CAS PubMed Google Scholar - Wiedmann, M., Kurzchalia, T. V., Bielka, H. & Rapoport, T. A. J. Cell Biol. 104, 201–208 (1987).
Article CAS Google Scholar - High, S., Görlich, D., Wiedmann, M., Rapoport, T. A. & Dobberstein, B. J. Cell Biol. 113, 35–44 (1991).
Article CAS Google Scholar - Brown, S. & Fournier, M. J. J. Cell Biol. 178, 533–550 (1984).
CAS Google Scholar - Randall, L. L. & Hardy, S. J. S. Science 243, 1156–1159 (1989).
Article ADS CAS Google Scholar - Kumamoto, C. A. Molec. Microbiol. 5, 19–22 (1991).
Article CAS Google Scholar - Langer, T. et al. Nature 356, 683–689 (1992).
Article ADS CAS Google Scholar - High, S., Flint, N. & Dobberstein, B. J. Cell Biol. 113, 25–34 (1991).
Article CAS Google Scholar - Römisch, K., Webb, J., Lingelbach, K., Gausepohl, H. & Dobberstein, B. J. Cell Biol. 111, 1793–1802 (1990).
Article Google Scholar - Walter, P. & Blobel, G. Meth. Enzym. 96, 682–691 (1983).
Article CAS Google Scholar
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Author notes
- Joen Luirink
Present address: Department of Molecular Microbiology, Faculty of Biology, De Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands
Authors and Affiliations
- European Molecular Biology Laboratory, Postfach 102209, Meyerhofstrasse 1, 6900, Heidelberg, Germany
Joen Luirink, Stephen High, Heather Wood, Angelika Giner, David Tollervey & Bernhard Dobberstein
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- Joen Luirink
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Luirink, J., High, S., Wood, H. et al. Signal-sequence recognition by an Escherichia coli ribonucleoprotein complex.Nature 359, 741–743 (1992). https://doi.org/10.1038/359741a0
- Received: 01 June 1992
- Accepted: 18 August 1992
- Issue Date: 22 October 1992
- DOI: https://doi.org/10.1038/359741a0