Genes required for GABA function in Caenorhabditis elegans (original) (raw)
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- Published: 22 July 1993
Nature volume 364, pages 334–337 (1993)Cite this article
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Abstract
γ-AMINOBITYRIC acid (GABA) neurotransmission is widespread in vertebrate and invertebrate nervous systems1. Here we use a genetic approach to identify molecules specific to GABA function. On the basis of the known in vivo roles of GABAergic neurons in controlling behaviour of the nematode _Caenorhabditis elegans_2, we identified mutants defective in GABA-mediated behaviours. Five genes are necessary either for GABAergic neuronal differentiation or for pre- or postsynaptic GABAergic function. The gene unc-30 is required for the differentiation of a specific type of GABAergic neuron, the type-D inhibitory motor neuron. The gene unc-25 is necessary for GABA expression and probably encodes the GABA biosynthetic enzyme glutamic acid decarboxylase. The genes unc-46 and unc-47 seem to be required for normal GABA release. Finally, the gene unc-49 is apparently necessary postsynaptically for the inhibitory effect of GABA on the body muscles and might encode a protein needed for the function of a GABAA-like receptor. Some of these genes are likely to encode previously unidentified proteins required for GABA function.
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Author notes
- Steven L. Mclntire
Present address: Department of Neurology, University of California, Box 0114, M-794, San Francisco, California, 94143-0114, USA - Steven L. Mclntire: Program in Neuroscience, Harvard Medical School, Boston, Massachusetts 02115, USA
Authors and Affiliations
- Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Room 56-629, 77 Massachusetts Avenue, Cambridge, Massachusetts, 02139, USA
Steven L. Mclntire, Erik Jorgensen & H. Robert Horvitz
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- Steven L. Mclntire
You can also search for this author inPubMed Google Scholar - Erik Jorgensen
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Mclntire, S., Jorgensen, E. & Horvitz, H. Genes required for GABA function in Caenorhabditis elegans.Nature 364, 334–337 (1993). https://doi.org/10.1038/364334a0
- Received: 09 March 1993
- Accepted: 28 April 1993
- Issue Date: 22 July 1993
- DOI: https://doi.org/10.1038/364334a0