Presynaptic A-current based on heteromultimeric K+ channels detected in vivo (original) (raw)

Nature volume 365, pages 72–75 (1993)Cite this article

Abstract

A WIDE variety of voltage-gated K+ channels are involved in the regulation of neuronal excitability and synaptic transmission. Their heterogeneity arises in part from the large number of genes encoding different K+ channel subunits (reviewed in ref. 1). In addition, heterologous expression studies indicate that assembly of distinct subunits into heteromultimeric channels may contribute further to K+ channel diversity2–6. A question has been whether heteromeric K+ channels actually form in vivo, and if so, whether specific combinations of subunits could account for major K+ currents identified in neurons. We present here biochemical evidence that Kvl.4 and Kvl.2, two K+ channel subunits of the Shaker subfamily, co-assemble in rat brain. The Kvl.4/Kvl.2 hetero-multimer combines features of both parent subunits, resulting in an A-type K+ channel6. Immunocytochemical evidence suggests that the heteromultimers are localized in axons and nerve terminals. We propose that Kvl.4/Kvl.2 heteromultimers may form the molecular basis of a presynaptic A-type K+ channel involved in the regulation of neurotransmitter release.

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References

  1. Jan, L. Y. & Jan, Y. N. Trends Neurosci. 13, 415–418 (1990).
    Article CAS Google Scholar
  2. Christie, M. J. et al. Neuron 4, 405–411 (1990).
    Article CAS Google Scholar
  3. Isacoff, E. Y., Jan, Y. N. & Jan, L. Y. Nature 345, 530–534 (1990).
    Article ADS CAS Google Scholar
  4. McCormack, J. W., Lin, L. E., Iverson, L. E. & Rudy, B. Biochem. biophys. Res. Commun. 171, 1361–1371 (1990).
    Article CAS Google Scholar
  5. Ruppersberg, J. P. et al. Nature 345, 535–537 (1990).
    Article ADS CAS Google Scholar
  6. Po, S. et al. Circulation Res. 72, 1326–1336 (1993).
    Article CAS Google Scholar
  7. Sheng, M., Tsaur, M.-L., Jan, Y. N. & Jan, L. Y. Neuron 9, 271–284 (1992).
    Article CAS Google Scholar
  8. Tsaur, M. L. et al. Neuron 8, 1055–1067 (1992).
    Article CAS Google Scholar
  9. Steward, O. & Scoville, S. A. J. comp. Neurol. 169, 347–376 (1976).
    Article CAS Google Scholar
  10. Baldwin, T. J. et al. Neuron 7, 471–483 (1991).
    Article CAS Google Scholar
  11. Covarrubias, M., Wei, A. & Salkoff, L. Neuron 7, 763–773 (1991).
    Article CAS Google Scholar
  12. Stühmer, W. et al. EMBO J. 8, 3235–3244 (1989).
    Article Google Scholar
  13. Rogawski, M. A. Trends Neurosci. 8, 214–219 (1985).
    Article Google Scholar
  14. Rudy, B. Neuroscience 25, 729–749 (1988).
    Article CAS Google Scholar
  15. Jan, Y. N., Jan, L. Y. & Dennis, M. Proc. R. Soc. Lond. B198, 87–108 (1977).
    ADS CAS PubMed Google Scholar
  16. Shimahara, T. Neurosci. Lett. 24, 139–142 (1981).
    Article CAS Google Scholar
  17. Kaang, B.-K. et al. Proc. natn. Acad. Sci. U.S.A. 89, 1133–1137 (1992).
    Article ADS CAS Google Scholar
  18. Tibbs, G. R., Dolly, J. O. & Nicholls, D. G. J. Neurochem. 52, 201–206 (1989).
    Article CAS Google Scholar

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Authors and Affiliations

  1. Departments of Physiology and Biochemistry, Howard Hughes Medical Institute, University of California, San Francisco, California, 94143, 0724, USA
    Morgan Sheng, Y. Joyce Liao, Yuh Nung Jan & Lily Yeh Jan

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  1. Morgan Sheng
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  2. Y. Joyce Liao
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  3. Yuh Nung Jan
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  4. Lily Yeh Jan
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Sheng, M., Liao, Y., Jan, Y. et al. Presynaptic A-current based on heteromultimeric K+ channels detected in vivo.Nature 365, 72–75 (1993). https://doi.org/10.1038/365072a0

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