A new pathway for synthesis of phosphatidylinositol-4,5-bisphosphate (original) (raw)
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- Published: 13 November 1997
Nature volume 390, pages 192–196 (1997)Cite this article
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Abstract
Phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2), a key molecule in the phosphoinositide signalling pathway, was thought to be synthesized exclusively by phosphorylation of PtdIns-4-P at the _D_-5 position of the inositol ring. The enzymes that produce PtdIns-4,5-P2 in vitro fall into two related subfamilies (type I and type II PtdInsP-5-OH kinases, or PIP(5)Ks) based on their enzymatic properties and sequence similarities1. Here we have reinvestigated the substrate specificities of these enzymes. As expected, the type I enzyme phosphorylates PtdIns-4-P at the _D_-5 position of the inositol ring. Surprisingly, the type II enzyme, which is abundant in some tissues, phosphorylates PtdIns-5-P at the _D_-4 position, and thus should be considered as a 4-OH kinase, or PIP(4)K. The earlier error in characterizing the activity of the type II enzyme is due to the presence of contaminating PtdIns-5-P in commercial preparations of PtdIns-4-P. Although PtdIns-5-P was previously thought not to exist in vivo, we find evidence for the presence of this lipid in mammalian fibroblasts, establishing a new pathway for PtdIns-4,5-P2 synthesis.
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Acknowledgements
We thank G. Preswich for synthetic PtdIns-5-P, K. Hinchliffe for recombinant type II PIPK, H. Ishihara for type I PIPK cDNA, K. Ravinchandran for the GST-SHIP expression vector, R. Meyers for recombinant PI-4Kβ, A. Couvillon for preparing recombinant type I PIPK and recombinant PI-3K, and D. Fruman and C. Carpenter for critically reading this manuscript, L.E.R. is supported by The Medical Foundation–Charles King Trust. This research was supported by the NIH.
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Authors and Affiliations
- Department of Cell Biology, and Division of Signal Transduction, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, 02115, Massachusetts, USA
Lucia E. Rameh, Kimberley F. Tolias, Brian C. Duckworth & Lewis C. Cantley
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- Lucia E. Rameh
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Correspondence toLucia E. Rameh.
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Rameh, L., Tolias, K., Duckworth, B. et al. A new pathway for synthesis of phosphatidylinositol-4,5-bisphosphate.Nature 390, 192–196 (1997). https://doi.org/10.1038/36621
- Received: 20 August 1997
- Accepted: 23 September 1997
- Issue Date: 13 November 1997
- DOI: https://doi.org/10.1038/36621