Host resistance to a fungal tomato pathogen lost by a single base-pair change in an avirulence gene (original) (raw)

Nature volume 367, pages 384–386 (1994)Cite this article

Abstract

HOST genotype specificity in interactions between biotrophic pathogens and plants in most cases complies with the gene-for-gene model1; success or failure of infection is determined by absence or presence of complementary genes, avirulence and resistance genes, in the pathogen and host plant, respectively. Resistance, expressed by the induction of a hypersensitive response in the host, is envisaged to be based on recognition of the pathogen, mediated through direct interaction between products of pathogen avirulence genes (the so-called race-specific elicitors) and receptors in the host plant, the putative products of resistance genes1. The interaction between the biotrophic fungus Cladosporium fulvum and its only host, tomato (Lycopersicon esculentum), is a well-established model system for studying fungus-plant gene-for-gene relationships1. Here we report the isolation of race-specific elicitor AVR4 of C. fulvum and the cloning of its encoding avirulence gene. We present evidence that, in nature, a single base-pair change in this avirulence gene leads to virulence of races previously avirulent on tomato genotypes carrying the complementary Cf4 resistance gene.

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References

  1. De Wit, P. J. G. M. A. Rev. Phytopath. 30, 391–418 (1992).
    Article CAS Google Scholar
  2. De Wit, P. J. G. M. & Spikman, G. Physiol. Pl Path. 21, 1–11 (1982).
    Article Google Scholar
  3. Von Heijne, G. Nucleic Acids Res. 14, 4683–4690 (1986).
    Article CAS Google Scholar
  4. Van Den Ackerveken, G. F. J. M., Vossen, P. & De Wit, P. J. G. M. Pl. Physiol. 103, 91–96 (1993).
    Article CAS Google Scholar
  5. Van Den Ackerveken, G. F. J. M., Van'Kan, J. A. L. & De Wit, P. J. G. M. Pl. J. 2, 359–366 (1992).
    Article CAS Google Scholar
  6. Van Kan, J. A. L., Van Den Ackerveken, G. F. J. M. & De Wit, P. J. G. M. Molec. Pl.-Microbe Interact. 4, 52–59 (1991).
    Article CAS Google Scholar
  7. Staskawicz, B. J., Dahlbeck, D. & Keen, N. T. Proc. natn. Acad. Sci. U.S.A. 81, 6024–6028 (1984).
    Article ADS CAS Google Scholar
  8. Kobayashi, D. J., Tamaki, S. J., Trollinger, D. J., Gold, S. & Keen, N. T. Molec. Pl.-Microbe Interact. 3, 103–111 (1990).
    Article CAS Google Scholar
  9. Herbers, K., Conrads-Strauch, J. & Bonas, U. Nature 356, 172–174 (1992).
    Article ADS CAS Google Scholar
  10. Kearney, B., Ronald, P. C., Dahlbeck, D. & Staskawicz, B. J. Nature 332, 541–543 (1988).
    Article ADS CAS Google Scholar
  11. Keen, N. T. A. Rev. Genet. 24, 447–463 (1990).
    Article CAS Google Scholar
  12. Joosten, M. H. A. J., Bergmans, C. J. B., Meulenhoff, E. J. S., Cornelissen, B. J. C. & De Wit, P. J. G. M. Pl. Physiol. 94, 585–591 (1990).
    Article CAS Google Scholar
  13. Van Kan, J. A. L., Joosten, M. H. A. J., Wagemakers, C. A. M., Van Den Berg-Velthuis, G. C. M. & De Wit, P. J. G. M. Pl.-molec. Biol. 20, 513–527 (1992).
    Article CAS Google Scholar

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Author notes

  1. Pierre J. G. M. De Wit: To whom correspondence is to be addressed.

Authors and Affiliations

  1. Department of Phytopathology, Wageningen Agricultural University, PO Box 8025, 6700 EE, Wageningen, The Netherlands
    Matthieu H. A. J. Joosten, Ton J. Cozijnsen & Pierre J. G. M. De Wit

Authors

  1. Matthieu H. A. J. Joosten
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  2. Ton J. Cozijnsen
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  3. Pierre J. G. M. De Wit
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Joosten, M., Cozijnsen, T. & De Wit, P. Host resistance to a fungal tomato pathogen lost by a single base-pair change in an avirulence gene.Nature 367, 384–386 (1994). https://doi.org/10.1038/367384a0

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