Changing subunit composition of heteromeric NMDA receptors during development of rat cortex (original) (raw)
- Letter
- Published: 10 March 1994
Nature volume 368, pages 144–147 (1994)Cite this article
- 3480 Accesses
- 1143 Citations
- 6 Altmetric
- Metrics details
Abstract
ACTIVATION of the _N_-methyl-d-aspartate (NMDA) receptor is important for certain forms of activity-dependent synaptic plasticity, such as long-term potentiation (reviewed in ref. 1), and the patterning of connections during development of the visual system (reviewed in refs 2, 3). Several subunits of the NMDA receptor have been cloned: these are NMDAR1 (NR1), and NMDAR2A, 2B, 2C and 2D (NR2A-D)4–8. Based on heterologous co-expression studies, it is inferred that NR1 encodes an essential subunit of NMDA receptors and that functional diversity of NMDA receptors in vivo is effected by differential incorporation of subunits NR2A–NR2D5–8. Little is known, however, about the actual subunit composition or heterogeneity of NMDA receptors in the brain. By co-immunoprecipitation with subunit-specific antibodies, we present here direct evidence that NMDA receptors exist in rat neocortex as heteromeric complexes of considerable heterogeneity, some containing both NR2A and NR2B subunits. A progressive alteration in subunit composition seen postnatally could contribute to NMDA-receptor variation and changing synaptic plasticity during cortical development.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
References
- Bliss, r. V. P. & Collingridge, G. L. Nature 361, 31–39 (1993).
Article ADS CAS Google Scholar - Constantine-Paton, M., Cline, H. T. & Debski, E. A. Rev. Neurosci. 13, 129–154 (1990).
Article CAS Google Scholar - Schatz, C. J. Neuron 5, 745–756 (1990).
Article Google Scholar - Moriyoshi, K. et al. Nature 354, 31–37 (1991).
Article ADS CAS Google Scholar - Monyer, H. et al. Science 256, 1217–1221 (1992).
Article ADS CAS Google Scholar - Meguro, H. et al. Nature 357, 70–74 (1992).
Article ADS CAS Google Scholar - Kutsuwada, T. et al. Nature 358, 36–41 (1992).
Article ADS CAS Google Scholar - Ishii, T. et al. J. biol. Chem. 268, 2836–2843 (1993).
CAS Google Scholar - Hollmann, M. et al. Neuron 10, 943–954 (1993).
Article CAS Google Scholar - Sugihara, H. et al. Biochem. biophys. Res. Commun. 185, 826–832 (1992).
Article CAS Google Scholar - Tingley, W. G., Roche, K. W., Thompson, A. K. & Huganir, R. L. Nature 364, 70–73 (1993).
Article ADS CAS Google Scholar - Chazot, P. L., Cik, M. & Stephenson, F. A. J. Neurochem. 59, 1176–1178 (1992).
Article CAS Google Scholar - Stern, P., Behe, P., Schoepfer, R. & Colquhoun, D. Proc. R. Soc. Lond. B250, 271–277 (1992).
Article ADS CAS Google Scholar - Carmignoto, G. & Vicini, S. Science 258, 1007–1011 (1992).
Article ADS CAS Google Scholar - Hestrin, S. Nature 357, 686–689 (1992).
Article ADS CAS Google Scholar - Ben-Ari, Y. et al. Neurosci. Lett. 94, 88–92 (1988).
Article CAS Google Scholar - Kato, N., Artoloa, A. & Singer, W. Devl. Brain Res. 60, 43–50 (1991).
Article CAS Google Scholar - Kleckner, N. W. & Dingledine, R. Molec. Brain Res. 11, 151–159 (1991).
CAS PubMed Google Scholar - Tsumoto, T., Hagihara, K., Sato, H. & Hata, Y. Nature 327, 513–514 (1987).
Article ADS CAS Google Scholar - Watanabe, M. et al. Neuroreport 3, 1138–1140 (1992).
Article CAS Google Scholar - Williams, K., Russell, S. L., Shen, Y. M. & Molinoff, P. B. Neuron 10, 267–278 (1993).
Article CAS Google Scholar - Kato, N. & Yoshimura, H. Proc. natn. Acad. Sci. U.S.A. 90, 7114–7118 (1993).
Article ADS CAS Google Scholar - Nakanishi, N., Axel, R. & Schneider, R. Proc. natn. Acad. Sci. U.S.A. 89, 8552–8556 (1992).
Article ADS CAS Google Scholar - Durand, G. M. et al. Proc. natn. Acad. Sci. U.S.A. 89, 9359–9363 (1992).
Article ADS CAS Google Scholar - Anantharam, V. et al. FEBS Lett. 305, 27–30 (1992).
Article CAS Google Scholar - Sheng, M., Tsaur, M-L., Jan, Y. N. & Jan, L. Y. Neuron 9, 271–284 (1992).
Article CAS Google Scholar - Sheng, M., Liao, Y. J., Jan, Y. N. & Jan, L. Y. Nature 365, 72–75 (1993).
Article ADS CAS Google Scholar - Tsaur, M-L., et al. Neuron 8, 1055–1067 (1992).
Article CAS Google Scholar
Author information
Authors and Affiliations
- Howard Hughes Medical Institute and Departments of Physiology and Biochemistry, University of California, San Francisco, California, 94143-0724, USA
Morgan Sheng, Jennifer Cummings, Leslie Ann Roldan, Yuh Nung Jan & Lily Yeh Jan
Authors
- Morgan Sheng
You can also search for this author inPubMed Google Scholar - Jennifer Cummings
You can also search for this author inPubMed Google Scholar - Leslie Ann Roldan
You can also search for this author inPubMed Google Scholar - Yuh Nung Jan
You can also search for this author inPubMed Google Scholar - Lily Yeh Jan
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Sheng, M., Cummings, J., Roldan, L. et al. Changing subunit composition of heteromeric NMDA receptors during development of rat cortex.Nature 368, 144–147 (1994). https://doi.org/10.1038/368144a0
- Received: 17 September 1993
- Accepted: 23 December 1993
- Issue Date: 10 March 1994
- DOI: https://doi.org/10.1038/368144a0