Bcl-2 expression promotes B- but not T-lymphoid development in scid mice (original) (raw)
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- Published: 31 March 1994
Nature volume 368, pages 457–460 (1994)Cite this article
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Abstract
EXPRESSION of antigen receptors is vital for the development of B and T lymphocytes. In mice with the scid mutation1,2, which are unable to make productive rearrangements of their immuno-globulin and T-cell receptor (TCR) genes, lymphopoiesis aborts at an early stage. The death of the immature lymphocytes by apoptosis3 is postulated to result from a failure to receive a survival signal induced by receptor engagement4. Consistent with this hypothesis, introduction of immunoglobulin or TCR transgenes into scid mice promoted an increase in B- or T-lymphoid cells, respectively5–7. As the protein encoded by the bcl-2 gene can inhibit cell death8,9, we tested whether lymphopoiesis could be rescued in scid mice by crossing in a bcl-2 transgene. Strikingly, the bcl-2 scid mice accumulated almost normal numbers of B-lymphoid cells which lacked surface immunoglobulin but expressed markers of maturity. T-cell development remained blocked. Introducing a TCR transgene enabled bcl-2/scid mice to develop normal numbers of CD4+8+ thymocytes even in the absence of immunological selection, suggesting that T cells become competent to respond to bcl-2 protein only after the TCR complex is displayed at the cell surface.
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References
- Bosma, C. G., Custer, R. P. & Bosma, M. J. Nature 301, 527–530 (1983).
Article ADS CAS Google Scholar - Bosma, M. J. & Carroll, A. M. A. Rev. Immun. 9, 323–350 (1991).
Article CAS Google Scholar - Osmond, D. G. et al. Blood 79, 1695–1703 (1992).
CAS PubMed Google Scholar - Rolink, A. & Melchers, F. Cell 66, 1081–1094 (1991).
Article CAS Google Scholar - Reichman-Fried, M., Hardy, R. R. & Bosma, M. J. Proc. natn. Acad. Sci. U.S.A. 87, 2730–2734 (1990).
Article ADS CAS Google Scholar - von Boehmer, H. A. Rev. Immun. 8, 531–556 (1990).
Article CAS Google Scholar - Scott, B., Blüthmann, H., Teh, H. S. & von Boehmer, H. Nature 338, 591–593 (1989).
Article ADS CAS Google Scholar - Vaux, D. L., Cory, S. & Adams, J. M. Nature 335, 440–442 (1988).
Article ADS CAS Google Scholar - Nuñez, G. et al. J. Immun. 144, 3602–3610 (1990).
PubMed Google Scholar - McDonnell, T. J. et al. Cell 57, 79–88 (1989).
Article CAS Google Scholar - Strasser, A. et al. Proc. natn. Acad. Sci. U.S.A. 88, 8661–8665 (1991).
Article ADS CAS Google Scholar - Strasser, A., Harris, A. W. & Cory, S. Cell 67, 889–899 (1991).
Article CAS Google Scholar - Sentman, C. L., Shutter, J. R., Hockenbery, D., Kanagawa, O. & Korsmeyer, S. J. Cell 67, 879–888 (1991).
Article CAS Google Scholar - Hardy, R. R., Kemp, J. D. & Hayakawa, K. Curr. Top. Microbiol. Immun. 152, 19–25 (1989).
CAS Google Scholar - Li, Y.-S., Hayakawa, K. & Hardy, R. R. J. exp. Med. 178, 951–960 (1993).
Article CAS Google Scholar - Muller-Sieburg, C. E., Whitlock, C. A. & Weissman, I. L. Cell 44, 653–662 (1986).
Article CAS Google Scholar - Ogawa, M. et al. J. exp. Med. 174, 63–71 (1991).
Article CAS Google Scholar - Waldschmidt, T. J., Conrad, D. H. & Lynch, R. G. J. Immun. 140, 2148–2154 (1988).
CAS PubMed Google Scholar - Kinoshita, T. et al. J. Immun. 140, 3066–3072 (1988).
CAS Google Scholar - Torres, R. M. et al. J. Immun. 149, 2641–2649 (1992).
CAS PubMed Google Scholar - Pennycook, J. L., Chang, Y., Celler, J., Phillips, R. A. & Wu, G. E. J. exp. Med. 178, 1007–1016 (1993).
Article CAS Google Scholar - Liu, Y. J. et al. Eur. J. Immun. 21, 1905–1910 (1991).
Article CAS Google Scholar - Strasser, A., Harris, A. W., von Boehmer, H. & Cory, S. Proc. natn. Acad. Sci. U.S.A. 91, 1376–1380 (1994).
Article ADS CAS Google Scholar - Reth, M. A. Rev. Immun. 10, 97–121 (1992).
Article CAS Google Scholar - Melchers, F. et al. Immun. Today 14, 60–68 (1993).
Article CAS Google Scholar - Groettrup, M. et al. Cell 75, 283–294 (1993).
Article CAS Google Scholar - Ashwell, J. D. & Klausner, R. D. A. Rev. Immun. 8, 531–556 (1990).
Article Google Scholar - Schlissel, M. S., Corcoran, L. M. & Baltimore, D. J. exp. Med. 173, 711–720 (1991).
Article CAS Google Scholar
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Authors and Affiliations
- The Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital, Victoria, 3050, Australia
Andreas Strasser, Alan W. Harris, Lynn M. Corcoran & Suzanne Cory
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- Andreas Strasser
You can also search for this author inPubMed Google Scholar - Alan W. Harris
You can also search for this author inPubMed Google Scholar - Lynn M. Corcoran
You can also search for this author inPubMed Google Scholar - Suzanne Cory
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Strasser, A., Harris, A., Corcoran, L. et al. Bcl-2 expression promotes B- but not T-lymphoid development in scid mice.Nature 368, 457–460 (1994). https://doi.org/10.1038/368457a0
- Received: 13 October 1993
- Accepted: 01 February 1994
- Issue Date: 31 March 1994
- DOI: https://doi.org/10.1038/368457a0