Cdk-activating kinase complex is a component of human transcription factor TFIIH (original) (raw)
- Letter
- Published: 16 March 1995
- Fred Mermelstein1,
- Robert P. Fisher2,
- Ronny Drapkin1,
- Brian Dynlacht3,
- Holly C. Wessling2,
- David O. Morgan2 &
- …
- Danny Reinberg1
Nature volume 374, pages 283–287 (1995)Cite this article
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Abstract
TRANSCRIPTION factor IIH (TFIIH) contains a kinase capable of phosphorylating the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (RNAPII)1–3. Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography. We find that affinity-purified antibodies directed against cyclin H inhibit TFIIH-dependent transcription and that both cyclin H and Cdk7 antibodies inhibit phosphorylation of the CTD of the largest subunit of the RNAPII in the preinitiation complex. Cak is present in at least two distinct complexes, TFIIH and a smaller complex that is unable to phosphorylate RNAPII in the preinitiation complex. Both Cak complexes, as well as recombinant Cak, phosphorylate a CTD peptide. Finally, TFIIH was shown to phosphorylate both Cdc2 and Cdk2, suggesting that there could be a link between transcription and the cell cycle machinery.
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References
- Lu H., Zawel, L., Fisher, L., Egly, J. M. & Reinberg, D. Nature 358, 641–645 (1992).
Article ADS CAS Google Scholar - Feaver, W. J., Gileadi, O., Li, Y. & Kornberg, R. D. Cell 67, 1223–1230 (1991).
Article CAS Google Scholar - Serizawa, H., Conaway, R. C. & Conaway, J. W. Proc. natn. Acad. Sci. U.S.A. 89, 7476–7460 (1992).
Article ADS CAS Google Scholar - Cisek, L. J. & Corden, J. L. Nature 339, 679–684 (1989).
Article ADS CAS Google Scholar - Cisek, L. J. & Corden, J. L. Meth. Enzym. 200, 301–325 (1991).
Article CAS Google Scholar - Zawel, L., Lu, H., Cisek, L. J., Corden, J. L. & Reinberg, D. Cold Spring Harbor Symp. quant. Biol. 58, 187–198 (1993).
Article CAS Google Scholar - Makela, T. P. et al. Nature 371, 254–257 (1994).
Article ADS CAS Google Scholar - Fisher, R. P. & Morgan, D. O. Cell 78, 713–724 (1994).
Article CAS Google Scholar - Lu, H., Flores, O., Weinmann, R. & Reinberg, D. Proc. natn. Acad. Sci. U.S.A. 88, 10004–10008 (1991).
Article ADS CAS Google Scholar - Drapkin, R. et al. Nature 368, 769–772 (1994).
Article ADS CAS Google Scholar - Van Vuuren, A. J. et al. EMBO J. 13, 1645–1653 (1994).
Article CAS Google Scholar - Wang, Z. et al. Nature 368, 74–75 (1994).
Article ADS CAS Google Scholar - Harlow, E. & Lane, D. Antibodies: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1988).
Google Scholar - Dignam, D. J., Lebowitz, R. M. & Roeder, R. G. Nucleic Acids Res. 11, 1475–1485 (1983).
Article CAS Google Scholar - Flores, O., Lu, H. & Reinberg, D. J. biol. Chem. 267, 2786–2793 (1992).
CAS Google Scholar - Roy, R. et al. Cell 79, 1093–1101 (1994).
Article CAS Google Scholar - Feaver, W. J., Svejstrup, J. Q., Henry, N. L. & Kornberg, R. D. Cell 79, 1103–1109 (1994).
Article CAS Google Scholar
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Authors and Affiliations
- Howard Hughes Medical Institute, Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane, Piscataway, New Jersey, 08854-5635, USA
Ramin Shiekhattar, Fred Mermelstein, Ronny Drapkin & Danny Reinberg - Department of Physiology, University of California, San Francisco, California, 94143-0444, USA
Robert P. Fisher, Holly C. Wessling & David O. Morgan - Laboratory of Molecular Oncology, The MGH Cancer Center, Building 149, 13th Street, Charlestown, Massachusetts, 02129, USA
Brian Dynlacht
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- Ramin Shiekhattar
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Shiekhattar, R., Mermelstein, F., Fisher, R. et al. Cdk-activating kinase complex is a component of human transcription factor TFIIH.Nature 374, 283–287 (1995). https://doi.org/10.1038/374283a0
- Received: 07 December 1994
- Accepted: 06 February 1995
- Issue Date: 16 March 1995
- DOI: https://doi.org/10.1038/374283a0