The role of neuropeptide Y in the antiobesity action of the obese gene product (original) (raw)

Nature volume 377, pages 530–532 (1995)Cite this article

Abstract

RECENTLY Zhang et al.1 cloned a gene that is expressed only in adipose tissue of the mouse. The obese phenotype of the ob/ob mouse is linked to a mutation in the obese gene that results in expression of a truncated inactive protein. Human and rat homolo-gues for this gene are known1,2. Previous experiments3,4 predict such a hormone to have a hypothalamic target. Hypothalamic neuropeptide Y stimulates food intake, decreases thermogenesis, and increases plasma insulin and corticosterone levels making it a potential target5. Here we express the obese protein in Escherichia coli and find that it suppresses food intake and decreases body weight dramatically when administered to normal and ob/ob mice but not db/db (diabetic) mice, which are thought to lack the appropriate receptor. High-affinity binding was detected in the rat hypo-thalamus. One mechanism by which this protein regulated food intake and metabolism was inhibition of neuropeptide-Y synthesis and release.

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Authors and Affiliations

  1. Endocrine Research and Technology Core Divisions, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana, 46285, USA
    Thomas W. Stephens, Margret Basinski, Pamela K. Bristow, Juliana M. Bue-Valleskey, Stanley G. Burgett, Libbey Craft, John Hale, James Hoffmann, Hansen M. Hsiung, Aidas Kriauciunas, Warren MacKellar, Paul R. Rosteck Jr, Brigitte Schoner, Dennis Smith, Frank C. Tinsley, Xing-Yue Zhang & Mark Heiman

Authors

  1. Thomas W. Stephens
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  2. Margret Basinski
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  3. Pamela K. Bristow
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  4. Juliana M. Bue-Valleskey
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  5. Stanley G. Burgett
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  6. Libbey Craft
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  7. John Hale
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  8. James Hoffmann
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  9. Hansen M. Hsiung
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  10. Aidas Kriauciunas
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  11. Warren MacKellar
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  12. Paul R. Rosteck Jr
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  13. Brigitte Schoner
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  14. Dennis Smith
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  15. Frank C. Tinsley
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  16. Xing-Yue Zhang
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  17. Mark Heiman
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Stephens, T., Basinski, M., Bristow, P. et al. The role of neuropeptide Y in the antiobesity action of the obese gene product.Nature 377, 530–532 (1995). https://doi.org/10.1038/377530a0

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