RNA editing of hepatitis delta virus antigenome by dsRNA-adenosine deaminase (original) (raw)

Nature volume 380, pages 454–456 (1996)Cite this article

An Erratum to this article was published on 23 May 1996

Abstract

HEPATITIS delta virus (HDV) is a subviral human pathogen that requires hepatitis B virus (HBV) for packaging1,2. Concurrent infection by HBV and HDV increases the risk of severe liver disease compared to infection with HBV alone3. The HDV genome is a closed circular RNA of about 1,700 bases which is replicated through an RNA intermediate, the antigenome4. Both RNAs can be folded into highly base-paired, rod-shaped structures, similar to the plant viroid RNAs5. Two forms of the sole HDV protein, hepatitis delta antigen, are derived from a single open reading frame by RNA editing; the enzymes responsible for the editing have not been characterized. Here we report that the purified enzyme dsRAD (for double-stranded-RNA-adenosine deaminase) can edit HDV antigenomic RNA in vitro. Most important, we observe that mutations in critical sequences of the antigenome have identical effects on in vitro and in vivo editing, suggesting that dsRAD, or a closely related enzyme, is responsible for editing HDV RNA in vivo.

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Author notes

  1. John L. Casey: Georgetown University Medical Center, Division of Molecular Virology and Immunology, 5640 Fisher's Lane, Rockville, Maryland 20852, USA

Authors and Affiliations

  1. Department of Biochemistry and Howard Hughes Medical Institute, 6110a EIHG, Building 533, University of Utah, Salt Lake City, Utah, 84112, USA
    Andrew G. Poison, Brenda L. Bass & John L. Casey

Authors

  1. Andrew G. Poison
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  2. Brenda L. Bass
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  3. John L. Casey
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Poison, A., Bass, B. & Casey, J. RNA editing of hepatitis delta virus antigenome by dsRNA-adenosine deaminase.Nature 380, 454–456 (1996). https://doi.org/10.1038/380454a0

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