Simultaneous independent measurement of endocytosis and exocytosis (original) (raw)
- Letter
- Published: 11 April 1996
Nature volume 380, pages 531–534 (1996)Cite this article
- 726 Accesses
- 204 Citations
- Metrics details
Abstract
STUDIES of membrane traffic between the cytoplasm and surface of a cell suggest that membrane internalization is tightly coupled to secretion. Studies using the capacitance technique show that endocytosis can follow evoked exocytosis within a second or less1–3. The capacitance technique, however, measures only the net change in cell surface area, and thus separating exocytosis from endocytosis requires that the two events do not overlap in time. This condition is probably met with small, brief stimuli1–5, but during prolonged stimulation it is more likely that exocytosis and endocytosis occur simultaneously6. We used FM1-43 fluorescence, which provides a cumulative measure of exocytosis, independent of endocytosis, in combination with capacitance monitoring to track unidirectional movements of membrane simultaneously and in real time in bovine adrenal chromaffin cells. We confirm that, with relatively small stimuli, exocytosis ceases before endocytosis begins (no overlap). In contrast, during prolonged stimulation, the onset of endocytosis is delayed by 2–3 min, but then the rate of endocytosis quickly grows to equal that of exocytosis. The delayed onset of endocytosis may be an emergency defence against catastrophic cell swelling.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
References
- Neher, E. & Zucker, R. Neuron 10, 21–30 (1993).
Article CAS Google Scholar - Thomas, P., Lee, A. K., Wong, J. G. & Aimers, W. J. Cell Biol. 124, 667–675 (1994).
Article CAS Google Scholar - Artalejo, C. R., Henley, J. R., McNiven, M. A. & Palfrey, H. C. Proc. natn. Acad. Sci. U.S.A. 92, 8328–8332 (1995).
Article ADS CAS Google Scholar - Artalejo, C. R., Elhamdani, A. & Palfrey, H. C. Neuron 16, 195–205 (1996).
Article CAS Google Scholar - von Gersdorff, H. & Mattherw, G. Nature 367, 735–739 (1994).
Article ADS CAS Google Scholar - Augustine, G. J. & Neher, E. J. Physiol. 450, 247–271 (1992).
Article CAS Google Scholar - Neher, E. & Marty, A. Proc. natn. Acad. Sci. U.S.A. 79, 6712–6716 (1982).
Article ADS CAS Google Scholar - Joshi, C. & Fernandez, J. M. Biophys. J. 53, 885–892 (1988).
Article CAS Google Scholar - Horrigan, F. T. & Bookman, R. J. Neuron 13, 1119–1129 (1994).
Article CAS Google Scholar - Burgoyne, R. D. Pflügers Arch. 430, 213–219 (1995).
Article CAS Google Scholar - Mollard, P., Seward, E. P. & Nowycky, M. C. Proc. natn. Acad. Sci. U.S.A. 92, 3065–3069 (1995).
Article ADS CAS Google Scholar - Heidelberger, R., Heinemann, C., Neher, E. & Matthews, G. Nature 371, 513–515 (1994).
Article ADS CAS Google Scholar - Parsons, T. D., Lenzi, D., Almers, W. & Roberts, W. M. Neuron 13, 875–883 (1994).
Article CAS Google Scholar - Rieke, F. & Schwartz, E. A. Neuron 13, 863–873 (1994).
Article CAS Google Scholar - Alvarezde Toledo, G., Fernandez-Chacon, R. & Fernandez, J. M. Nature 363, 554–558 (1993).
Article ADS CAS Google Scholar - Hartmann, J., Scepek, S. & Lindau, M. J. Physiol., Lond. 483, 201–209 (1995).
Article CAS Google Scholar - Betz, W. J. & Bewick, G. S. Science 255, 200–203 (1992).
Article ADS CAS Google Scholar - Betz, W. J., Mao, F. & Bewick, G. S. J. Neuroscience 12, 363–375 (1992).
Article CAS Google Scholar - Ryan, T. A. et al. Neuron 11, 713–724 (1993).
Article CAS Google Scholar - Betz, W. J. & Henkel, A. W. J. Cell Biol. 124, 843–854 (1994).
Article CAS Google Scholar - Kraszewski, K. et al. Neuroscience 15, 4328–4342 (1995).
Article CAS Google Scholar - Ryan, T. A. & Smith, S. J. Neuron 14, 983–989 (1995).
Article CAS Google Scholar - Henkel, A. W., Lübke, J. & Betz, W. J. Proc. natn. Acad. Sci. U.S.A. (in the press).
- Grinvald, A., Hildesheim, R., Farber, I. C. & Anglister, L. Biophys. J. 39, 301–308 (1982).
Article ADS CAS Google Scholar - Grinvald, A., Frostig, R. D., Lieke, E. & Hildesheim, R. Physiol. Rev. 68, 1285–1366 (1988).
Article CAS Google Scholar - Oberhauser, A. F. & Fernandez, J. M. Biophys. J. 69, 451–459 (1995).
Article ADS CAS Google Scholar - Greer, M. A., Hagemenas, F. C. & Illingsworth, D. R. Hormone Metab. Res. 24, (1992).
- Hoffmann, E. K. & Dunham, P. B. Int. Rev. Cytol. 161, 173–262 (1995).
Article CAS Google Scholar - Fidler, N. & Fernandez, J. M. Biophys. Journal 56, 1153–1162 (1989).
Article ADS CAS Google Scholar - Neher, E. J. Physiol., Lond. 395, 193–214 (1988).
Article ADS CAS Google Scholar
Author information
Authors and Affiliations
- Department of Physiology and Neuroscience Program, University of Colorado Medical School, Denver, Colorado, 80262, USA
C. B. Smith & W. J. Betz
Authors
- C. B. Smith
You can also search for this author inPubMed Google Scholar - W. J. Betz
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Smith, C., Betz, W. Simultaneous independent measurement of endocytosis and exocytosis.Nature 380, 531–534 (1996). https://doi.org/10.1038/380531a0
- Received: 31 January 1996
- Accepted: 04 March 1996
- Issue Date: 11 April 1996
- DOI: https://doi.org/10.1038/380531a0
This article is cited by
Transcellular propagation of fibrillar α-synuclein from enteroendocrine to neuronal cells requires cell-to-cell contact and is Rab35-dependent
- Paulla Vieira Rodrigues
- João Vitor Pereira de Godoy
- Matheus de Castro Fonseca
Scientific Reports (2022)
Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
- Agustina Pascual
- Emiliano S. Vilardo
- Rolando Rivera Pomar
Development Genes and Evolution (2021)
Effects of near infrared focused laser on the fluorescence of labelled cell membrane
- Remy Avila
- Elisa Tamariz
- Pablo Loza-Alvarez
Scientific Reports (2018)
v-SNARE function in chromaffin cells
- Madhurima Dhara
- Ralf Mohrmann
- Dieter Bruns
Pflügers Archiv - European Journal of Physiology (2018)
Regulation by L-Type Calcium Channels of Endocytosis: An Overview
- Juliana M. Rosa
- Carmen Nanclares
- Luis Gandía
Journal of Molecular Neuroscience (2012)