A human peptidyl–prolyl isomerase essential for regulation of mitosis (original) (raw)

Nature volume 380, pages 544–547 (1996)Cite this article

Abstract

THE NIMA kinase is essential for progression through mitosis in _Aspergillus nidulans_1–6, and there is evidence for a similar pathway in other eukaryotic cells5–8. Here we describe the human protein Pinl, a peptidyl–prolyl cis/trans isomerase (PPIase) that interacts with NIMA. PPIases are important in protein folding, assembly and/or transport9–13, but none has so far been shown to be required for cell viability9–11. Pinl is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Essl/Ptfl, an essential protein in budding yeast14,15. PPIase activity is necessary for Essl/Pinl function in yeast. Depletion of Pinl/Essl from yeast or HeLa cells induces mitotic arrest, whereas HeLa cells overexpressing Pinl arrest in the G2 phase of the cell cycle. Pinl is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity.

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References

  1. Osmani, S. A., Pu, R. T. & Morris, N. R. Cell 53, 237–244 (1988).
    Article CAS Google Scholar
  2. Osmani, A. H., McGuire, S. L. & Osmani, S. A. Cell 67, 283–291 (1991).
    Article CAS Google Scholar
  3. Lu, K. P. & Means, A. R. EMBO J. 13, 2103–2113 (1994).
    Article CAS Google Scholar
  4. Pu, R. T. & Osmani, S. A. EMBO J. 14, 995–1003 (1995).
    Article CAS Google Scholar
  5. Lu, K. P. & Hunter, T. in Progress in Cell Cycle Research Vol. 1 (eds Meijer, L., Guidet, S. & Tung, H. Y. L.) 187–205 (Plenum, New York, 1996).
    Google Scholar
  6. Fry, A. M. & Nigg, E. A. Curr. Biol. 5, 1122–1125 (1995).
    Article CAS Google Scholar
  7. Lu, K. P. & Hunter, T. Cell 81, 413–424 (1995).
    Article CAS Google Scholar
  8. O'Connell, M. J., Norbury, C. & Nurse, P. EMBO J. 13, 4926–4937 (1994).
    Article CAS Google Scholar
  9. Schreiber, S. L. Science 251, 283–287 (1991).
    Article ADS CAS Google Scholar
  10. Heitman, J., Mowa, N. R. & Hall, M. N. New Biol. 4, 448–460 (1992).
    CAS PubMed Google Scholar
  11. Fischer, G. Angew. Chem. int. Edn. engl. 63, 67–118 (1994).
    Google Scholar
  12. Rahfeld, J. U. et al. FEBS Lett. 343, 65–69 (1994).
    Article CAS Google Scholar
  13. Rudd, K. E. et al. Trends biochem. Sci. 20, 12–14 (1995).
    Article CAS Google Scholar
  14. Hanes, S. D., Shank, P. R. & Bostian, K. A. Yeast 5, 55–72 (1989).
    Article CAS Google Scholar
  15. Hani, J., Stumpf, G. & Domdey, H. FEBS Lett. 365, 198–202 (1995).
    Article CAS Google Scholar
  16. Sudol, M. et al. FEBS Lett. 369, 67–71 (1995).
    Article CAS Google Scholar
  17. Chen, H. I. & Sudol, M. Proc. natn. Acad. Sci. U.S.A. 92, 7819–7823 (1995).
    Article ADS CAS Google Scholar
  18. Davis, E. S. et al. Proc. natn. Acad. Sci. U.S.A. 89, 11169–11173 (1992).
    Article ADS CAS Google Scholar
  19. Fu, X. D. & Maniatis, T. Nature 343, 437–441 (1990).
    Article ADS CAS Google Scholar
  20. Spector, D. L. A. Rev. Cell Biol. 9, 265–315 (1993).
    Article CAS Google Scholar
  21. Fu, X. D. RNA 1, 663–680 (1995).
    CAS PubMed PubMed Central Google Scholar
  22. Takeuchi, M. & Yanagida, M. Molec. Biol. Cell 4, 247–260 (1993).
    Article CAS Google Scholar
  23. Gui, J. F., Lane, W. S. & Fu, X. D. Nature 369, 678–682 (1994).
    Article ADS CAS Google Scholar
  24. Maleszka, R. et al. Proc. natn. Acad. Sci. U.S.A. 93, 447–451 (1996).
    Article ADS CAS Google Scholar
  25. Harper, J. W. et al. Cell 75, 805–816 (1993).
    Article CAS Google Scholar
  26. Hannon, G. J., Demetrick, D. & Beach, D. Genes Dev. 7, 2378–2391 (1993).
    Article CAS Google Scholar
  27. Heitman, J. et al. Methods 5, 176–187 (1993).
    Article CAS Google Scholar
  28. Louvion, J. F., Havaux, C. B. & Picard, D. Gene 131, 129–134 (1993).
    Article CAS Google Scholar
  29. Roberts, C. J. et al. Meth. Enzym. 194, 644–661 (1991).
    Article CAS Google Scholar
  30. Lu, K. P., Osmani, S. A. & Means, A. R. J. biol. Chem. 268, 8769–8776 (1993).
    CAS PubMed Google Scholar

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Author notes

  1. Steven D. Hanes: Wadsworth Center, Laboratory of Developmental Genetics, New York State Department of Health, 120 New Scotland Avenue, Albany, New York 12201, USA

Authors and Affiliations

  1. Molecular Biology and Virology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, California, 92037, USA
    Kun Ping Lu, Steven D. Hanes & Tony Hunter

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  1. Kun Ping Lu
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  2. Steven D. Hanes
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  3. Tony Hunter
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Ping Lu, K., Hanes, S. & Hunter, T. A human peptidyl–prolyl isomerase essential for regulation of mitosis.Nature 380, 544–547 (1996). https://doi.org/10.1038/380544a0

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