A human exchange factor for ARF contains Sec7- and pleckstrin-homology domains (original) (raw)

Nature volume 384, pages 481–484 (1996)Cite this article

Abstract

THE small G protein ARF1 is involved in the coating of vesicles that bud from the Golgi compartments1,2. Its activation is controlled by as-yet unidentified guanine-nucleotide exchange factors3,4. Geal, the first ART exchange factor to be discovered in yeast5, is a large protein containing a domain of homology with Sec7, another yeast protein that is also involved in secretion6. Here we characterized a smaller human protein (relative molecular mass 47K) named ARNO, which contains a central Sec7 domain that promotes guanine-nucleotide exchange on ARF1. ARNO also contains an amino-terminal coiled-coil motif and a carboxy-terminal pleckstrin-homology (PH) domain. The PH domain mediates an enhancement of ARNO exchange activity by negatively charged phospholipid vesicles supplemented with phosphatidylinositol bisphosphate. The exchange activity of ARNO is not inhibited by brefeldin A, an agent known to block vesicular transport and inhibit the exchange activity on ARF1 in cell extracts7,8. This suggests that a regulatory component which is sensitive to brefeldin A associates with ARNO in vivo, possibly through the amino-terminal coiled-coil. We propose that other proteins with a Sec7 domain regulate different members of the ART family.

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Author notes

  1. Catherine L. Jackson: Service de Biochimie et de Génétique Moléculaire (Bat. 142), CEA, CEA/Saclay, 91191 Gif-sur-Yvette, France

Authors and Affiliations

  1. Institut de Pharmacologie Moléculaire et Cellulaire du CNRS, 660 route des Lucioles, 06560, Valbonne, France
    Pierre Chardin, Sonia Paris, Bruno Antonny, Sylviane Robineau, Sophie Béraud-Dufour, Catherine L. Jackson & Marc Chabre

Authors

  1. Pierre Chardin
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  2. Sonia Paris
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  3. Bruno Antonny
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  4. Sylviane Robineau
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  5. Sophie Béraud-Dufour
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  6. Catherine L. Jackson
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  7. Marc Chabre
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Chardin, P., Paris, S., Antonny, B. et al. A human exchange factor for ARF contains Sec7- and pleckstrin-homology domains.Nature 384, 481–484 (1996). https://doi.org/10.1038/384481a0

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