A human exchange factor for ARF contains Sec7- and pleckstrin-homology domains (original) (raw)
- Letter
- Published: 05 December 1996
- Sonia Paris1,
- Bruno Antonny1,
- Sylviane Robineau1,
- Sophie Béraud-Dufour1,
- Catherine L. Jackson1 na1 &
- …
- Marc Chabre1
Nature volume 384, pages 481–484 (1996)Cite this article
- 969 Accesses
- 408 Citations
- 6 Altmetric
- Metrics details
Abstract
THE small G protein ARF1 is involved in the coating of vesicles that bud from the Golgi compartments1,2. Its activation is controlled by as-yet unidentified guanine-nucleotide exchange factors3,4. Geal, the first ART exchange factor to be discovered in yeast5, is a large protein containing a domain of homology with Sec7, another yeast protein that is also involved in secretion6. Here we characterized a smaller human protein (relative molecular mass 47K) named ARNO, which contains a central Sec7 domain that promotes guanine-nucleotide exchange on ARF1. ARNO also contains an amino-terminal coiled-coil motif and a carboxy-terminal pleckstrin-homology (PH) domain. The PH domain mediates an enhancement of ARNO exchange activity by negatively charged phospholipid vesicles supplemented with phosphatidylinositol bisphosphate. The exchange activity of ARNO is not inhibited by brefeldin A, an agent known to block vesicular transport and inhibit the exchange activity on ARF1 in cell extracts7,8. This suggests that a regulatory component which is sensitive to brefeldin A associates with ARNO in vivo, possibly through the amino-terminal coiled-coil. We propose that other proteins with a Sec7 domain regulate different members of the ART family.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
References
- Schekman, R. & Orci, L. Science 271, 1526–1533 (1996).
Article CAS ADS Google Scholar - Rothman, J. E. & Wieland, F. T. Science 272, 227–234 (1996).
Article CAS ADS Google Scholar - Franco, M., Chardin, P., Chabre, M. & Paris, S. J. Biol. Chem. 271, 1573–1578 (1996).
Article CAS Google Scholar - Tsai, S.-C., Adamik, R., Moss, J. & Vaughan, M. Proc. Natl Acad. Sci. USA 91, 3063–3066 (1994).
Article CAS ADS Google Scholar - Peyroche, A., Paris, S. & Jackson, C. L. Nature 384, 479–481 (1996).
Article CAS ADS Google Scholar - Achstetter, T. et al. J. Biol. Chem. 263, 11711–11717 (1988).
CAS PubMed Google Scholar - Donaldson, J. G., Finazzi, D. & Klausner, R. D. Nature 360, 350–352 (1992).
Article CAS ADS Google Scholar - Helms, J. B. & Rothman, J. E. Nature 360, 352–354 (1992).
Article CAS ADS Google Scholar - Lennon, G. G., Auffray, C., Polymeropoulos, M. & Soares, M. B. Genomics 33, 151–152 (1996).
Article CAS Google Scholar - Shevell, D. E. et al. Cell 77, 1051–1062 (1994).
Article CAS Google Scholar - Sulston, J. et al. Nature 356, 37–41 (1992).
Article ADS Google Scholar - Liu, L. & Pohajdak, B. Biochim. Biophys. Acta 1132, 75–78 (1992).
Article CAS Google Scholar - Ferguson, K. M. et al. Cell 83, 1037–1046 (1995).
Article CAS Google Scholar - Hyvönen, M. et al. EMBO J. 14, 4676–4685 (1995).
Article Google Scholar - Porfiri, E., Evans, T., Chardin, P. & Hancock, J. F. J. Biol. Chem. 269, 2262–22677 (1994).
Google Scholar - Cerione, R. A. & Zheng, Y. Curr. Opin. Cell Biol. 8, 216–222 (1996).
Article CAS Google Scholar - Hammond, S. M. et al. J. Biol. Chem. 270, 29640–29643 (1995).
Article CAS Google Scholar - Tucker, J. et al. EMBO J. 5, 1351–1358 (1986).
Article CAS Google Scholar - MacDonald, R. C. et al. Biochim. Biophys. Acta 1061, 297–303 (1991).
Article CAS Google Scholar
Author information
Author notes
- Catherine L. Jackson: Service de Biochimie et de Génétique Moléculaire (Bat. 142), CEA, CEA/Saclay, 91191 Gif-sur-Yvette, France
Authors and Affiliations
- Institut de Pharmacologie Moléculaire et Cellulaire du CNRS, 660 route des Lucioles, 06560, Valbonne, France
Pierre Chardin, Sonia Paris, Bruno Antonny, Sylviane Robineau, Sophie Béraud-Dufour, Catherine L. Jackson & Marc Chabre
Authors
- Pierre Chardin
You can also search for this author inPubMed Google Scholar - Sonia Paris
You can also search for this author inPubMed Google Scholar - Bruno Antonny
You can also search for this author inPubMed Google Scholar - Sylviane Robineau
You can also search for this author inPubMed Google Scholar - Sophie Béraud-Dufour
You can also search for this author inPubMed Google Scholar - Catherine L. Jackson
You can also search for this author inPubMed Google Scholar - Marc Chabre
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Chardin, P., Paris, S., Antonny, B. et al. A human exchange factor for ARF contains Sec7- and pleckstrin-homology domains.Nature 384, 481–484 (1996). https://doi.org/10.1038/384481a0
- Received: 25 June 1996
- Accepted: 07 September 1996
- Issue Date: 05 December 1996
- DOI: https://doi.org/10.1038/384481a0