RNA interference in adult mice (original) (raw)

Gene expression

Nature volume 418, pages 38–39 (2002)Cite this article

Abstract

RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Figure 1: RNA interference in adult mice.

Similar content being viewed by others

References

  1. Elbashir, S. M. et al. Nature 411, 494–498 (2001).
    Article ADS CAS Google Scholar
  2. Zhang, G., Budker, V. & Wolff, J. A. Hum. Gene Therapy 10, 1735–1737 (1999).
    Article CAS Google Scholar
  3. Liu, F., Song, Y. & Liu, D. Gene Therapy 6, 1258–1266 (1999).
    Article CAS Google Scholar
  4. Chang, J., Sigal, L. J., Lerro, A. & Taylor, J. J. Virol. 75, 3469–3473 (2001).
    Article CAS Google Scholar
  5. Contag, C. H. et al. Photochem. Photobiol. 66, 523–531 (1997).
    Article CAS Google Scholar
  6. Yant, S. R. et al. Nature Genet. 25, 35–41 (2000).
    Article CAS Google Scholar
  7. Bitko, V. & Barik, S. BMC Microbiol. 1, 34 (2001).
    Article CAS Google Scholar
  8. Paddison, P. J., Caudy, A. A., Bernstein, E., Hannon, G. J. & Conklin, D. S. Genes Dev. 16, 948–958 (2002).
    Article CAS Google Scholar
  9. Tuschl, T. Nature Biotechnol. 20, 446–448 (2002).
    Article CAS Google Scholar

Download references

Author information

Authors and Affiliations

  1. Departments of Pediatrics and Genetics, Stanford University School of Medicine, Stanford, 94305-5208, California, USA
    Anton P. McCaffrey, Leonard Meuse, Thu-Thao T. Pham & Mark A. Kay
  2. Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, 11724, New York, USA
    Douglas S. Conklin & Gregory J. Hannon

Authors

  1. Anton P. McCaffrey
    You can also search for this author inPubMed Google Scholar
  2. Leonard Meuse
    You can also search for this author inPubMed Google Scholar
  3. Thu-Thao T. Pham
    You can also search for this author inPubMed Google Scholar
  4. Douglas S. Conklin
    You can also search for this author inPubMed Google Scholar
  5. Gregory J. Hannon
    You can also search for this author inPubMed Google Scholar
  6. Mark A. Kay
    You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toMark A. Kay.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Rights and permissions

About this article

Cite this article

McCaffrey, A., Meuse, L., Pham, TT. et al. RNA interference in adult mice.Nature 418, 38–39 (2002). https://doi.org/10.1038/418038a

Download citation

This article is cited by