Maturation of mouse fetal germ cells in vitro (original) (raw)

• Brief Communication
• Published: 01 August 2002

Oogenesis

Nature volume 418, page 497 (2002)Cite this article

• 2373 Accesses
• 89 Citations
• Metrics details

Even immature oocytes can eventually be fertilized after some skilful manipulation.

Abstract

Nuclear reprogramming is essential during gametogenesis for the production of totipotent zygotes. Here we show that premeiotic female germ cells derived from mouse fetuses as early as 12.5 days post coitum are able to complete meiosis and genomic imprinting in vitro and that these matured oocytes are highly competent in supporting development to full term after nuclear transfer and in vitro fertilization. To our knowledge, this is the first time that complete oogenesis has been successfully accomplished in vitro.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

• Purchase on SpringerLink
• Instant access to full article PDF

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

• Log in
• Learn about institutional subscriptions
• Read our FAQs
• Contact customer support

Figure 1: Development of premeiotic female germ cells in vitro.

Similar content being viewed by others

References

1. Buehr, M. & McLaren, A. Gamete Res. 11, 271–281 (1985).
   Article CAS Google Scholar
2. Eppig, J. J. & O'Brien, M. J. Biol. Reprod. 54, 197–207 (1996).
   Article CAS Google Scholar
3. Cortvrindt, R., Smitz, J. & Van Steirteghem, A. C. Hum. Reprod. 11, 2656–2666 (1996).
   Article CAS Google Scholar
4. Bao, S., Obata, Y., Carroll, J., Domeki, I. & Kono, T. Biol. Reprod. 62, 616–621 (2000).
   Article CAS Google Scholar
5. Kono, T., Obata, Y., Yoshimzu, T., Nakahara, T. & Carroll, J. Nature Genet. 13, 91–94 (1996).
   Article CAS Google Scholar
6. Obata, Y. et al. Development 125, 1553–1560 (1998).
   CAS PubMed Google Scholar
7. Obata, Y. & Kono, T. J. Biol. Chem. 277, 5285–5289 (2002).
   Article CAS Google Scholar
8. Wutz, A. et al. Nature 389, 745–749 (1997).
   Article ADS CAS Google Scholar
9. Bourc'his, D., Xu, G. L., Lin, C. S., Bollman, B. & Bestor, T. H. Science 294, 2536–2539 (2001).
   Article ADS CAS Google Scholar
10. Lucifero, D., Mertineit, C., Clarke, H. J., Bestor, T. H. & Trasler, J. M. Genomics 79, 530–538 (2002).
    Article CAS Google Scholar
11. Okabe, M., Ikawa, M., Kominami, K., Nakanishi, T. & Nishimune, Y. FEBS Lett. 407, 313–319 (1997).
    Article CAS Google Scholar

Download references

Author information

Authors and Affiliations

1. Gene Research Center, Gunma University, 3-39-22 Showa-machi, Maebashi, 371-8511, Gunma, Japan
   Yayoi Obata & Izuho Hatada
2. Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, 156-8502, Japan
   Tomohiro Kono

Authors

1. Yayoi Obata
2. Tomohiro Kono
3. Izuho Hatada

Corresponding author

Correspondence toIzuho Hatada.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Rights and permissions

About this article

Cite this article

Obata, Y., Kono, T. & Hatada, I. Maturation of mouse fetal germ cells in vitro.Nature 418, 497 (2002). https://doi.org/10.1038/418497a

Download citation

• Issue Date: 01 August 2002
• DOI: https://doi.org/10.1038/418497a

This article is cited by