Generalized lacZ expression with the ROSA26 Cre reporter strain (original) (raw)

Nature Genetics volume 21, pages 70–71 (1999)Cite this article

Mouse strains expressing the site-specific recombinase Cre (or Flp) facilitate conditional ablation of gene function when one or several exons of the gene of interest are flanked by loxP (or FRT) sites1. Cre expression achieved by classic transgenesis or targeting to an appropriate locus might be tissue specific, temporally restricted or inducible2,3. In such experimental outlines, it is necessary to monitor Cre activity at desired time points as well as to verify that Cre was not active previously during development. Other investigators have generated transgenic4,5 or knock-in6 lines in which lacZ expression is conditional on the removal of an intervening segment. However, such lines are most useful if lacZ can be expressed in all cell types and hence is driven off a constitutively active promoter in the mouse.

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Acknowledgements

I thank C. Auger, P. Capp and K. Weismann for technical assistance. This work was supported by NIH grant RO1 HD 24875.

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  1. Division of Basic Sciences, Program in Developmental Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North , Seattle, 98109, Washington, USA
    Philippe Soriano

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  1. Philippe Soriano
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Soriano, P. Generalized lacZ expression with the ROSA26 Cre reporter strain .Nat Genet 21, 70–71 (1999). https://doi.org/10.1038/5007

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