Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogene (original) (raw)

Nature Genetics volume 25, pages 144–146 (2000)Cite this article

Abstract

We show here that quantitative measurement of DNA copy number across amplified regions using array comparative genomic hybridization1,2,3,4 (CGH) may facilitate oncogene identification by providing precise information on the locations of both amplicon boundaries and amplification maxima. Using this analytical capability, we resolved two regions of amplification within an approximately 2-Mb region of recurrent aberration at 20q13.2 in breast cancer. The putative oncogene ZNF217 (ref. 5) mapped to one peak, and CYP24 (encoding vitamin D 24 hydroxylase), whose overexpression is likely to lead to abrogation of growth control mediated by vitamin D (ref. 6), mapped to the other.

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Acknowledgements

This work was supported by NIH grants CA80314, CA45919, HD17665 and P50 CA58207, California BCRP grant 2RB-0225 and Vysis.

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Authors and Affiliations

  1. Cancer Research Institute, University of California, San Francisco, Box 0808, San Francisco, USA, California
    Donna G. Albertson, Bauke Ylstra, Colin Collins & David Kowbel
  2. Cancer Genetics and Breast Oncology Programs, UCSF Cancer Center, University of California, San Francisco, Box 0808, San Francisco, USA, California
    Donna G. Albertson, Richard Segraves, Colin Collins, Wen-Lin Kuo, Joe W. Gray & Daniel Pinkel
  3. Geraldine Brush Cancer Research Institute, California Pacific Medical Center, San Francisco, California, USA
    Shanaz H. Dairkee

Authors

  1. Donna G. Albertson
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  2. Bauke Ylstra
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  3. Richard Segraves
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  4. Colin Collins
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  5. Shanaz H. Dairkee
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  6. David Kowbel
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  7. Wen-Lin Kuo
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  8. Joe W. Gray
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  9. Daniel Pinkel
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Corresponding author

Correspondence toDonna G. Albertson.

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Albertson, D., Ylstra, B., Segraves, R. et al. Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogene.Nat Genet 25, 144–146 (2000). https://doi.org/10.1038/75985

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