Bactericidal antisense effects of peptide–PNA conjugates (original) (raw)

References

Davies, J. Inactivation of antibiotics and the dissemination of resistance genes. Science 264, 375–382 (1994).
Article CAS Google Scholar
Tan, Y.T., Tillett, D.J. & McKay, I.A. Molecular strategies for overcoming antibiotic resistance in bacteria. Mol. Med. Today 6, 309–314 (2000).
Article CAS Google Scholar
Murray, R.W., Schaadt, R.D., Zurenko, G.E. & Marotti, K.R. Ribosomes from an oxazolidinone-resistant mutant confer resistance to eperezolid in a Staphylococcus aureus cell-free transcription–translation assay. Antimicrob. Agents Chemother. 42, 947–950 (1998).
Article CAS Google Scholar
Good, L. & Nielsen, P.E. Antisense inhibition of gene expression in bacteria by PNA targeted to mRNA. Nat. Biotechnol. 16, 355–358 (1998).
Article CAS Google Scholar
Good, L. & Nielsen, P.E. Inhibition of translation and bacterial growth by peptide nucleic acid targeted to ribosomal RNA. Proc. Natl. Acad. Sci. USA 95, 2073–2076 (1998).
Article CAS Google Scholar
White, D.G. et al. Inhibition of the multiple antibiotic resistance (mar) operon in Escherichia coli by antisense DNA analogs. Antimicrob. Agents Chemother. 41, 2699–2704 (1997).
Article CAS Google Scholar
Harth, G., Zamecnik, P.C., Tang, J.Y., Tabatadze, D. & Horwitz, M.A. Treatment of Mycobacterium tuberculosis with antisense oligonucleotides to glutamine synthetase mRNA inhibits glutamine synthetase activity, formation of the poly-l-glutamate/glutamine cell wall structure, and bacterial replication. Proc. Natl. Acad. Sci. USA 97, 418–423 (2000).
Article CAS Google Scholar
Nielsen, P.E., Egholm, M., Berg, R.H. & Buchardt, O. Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide. Science 254, 1497–1500 (1991).
Article CAS Google Scholar
Wittung, P., Nielsen, P.E., Buchardt, O., Egholm, M. & Norden, B. DNA-like double helix formed by peptide nucleic acid. Nature 368, 561–563 (1994).
Article CAS Google Scholar
Jensen, K.K., Orum, H., Nielsen, P.E. & Norden, B. Kinetics for hybridization of peptide nucleic acids (PNA) with DNA and RNA studied with the BIAcore technique. Biochemistry 36, 5072–5077 (1997).
Article CAS Google Scholar
Egholm, M. et al. PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rules. Nature 365, 566–568 (1993).
Article CAS Google Scholar
Giesen, U. et al. A formula for thermal stability (Tm) prediction of PNA/DNA duplexes. Nucleic Acids Res. 26, 5004–5006 (1998).
Article CAS Google Scholar
Demidov, V.V. et al. Stability of peptide nucleic acids in human serum and cellular extracts. Biochem. Pharmacol. 48, 1310–1313 (1994).
Article CAS Google Scholar
Nielsen, P.E., Haaima, G. Peptide nucleic acid (PNA). A DNA mimic with a pseudopeptide backbone. Chem. Soc. Rev. 96, 73–78 (1997).
Article Google Scholar
Hanvey, J.C. et al. Antisense and antigene properties of peptide nucleic acids. Science 258, 1481–1485 (1992).
Article CAS Google Scholar
Knudsen, H. & Nielsen, P.E. Antisense properties of duplex- and triplex-forming PNAs. Nucleic Acids Res. 24, 494–500 (1996).
Article CAS Google Scholar
Norton, J.C., Piatyszek, M.A., Wright, W.E., Shay, J.W. & Corey, D.R. Inhibition of human telomerase activity by peptide nucleic acids. Nat. Biotechnol. 14, 615–619 (1996).
Article CAS Google Scholar
Taylor, R.W., Chinnery, P.F., Turnbull, D.M. & Lightowlers, R.N. Selective inhibition of mutant human mitochondrial DNA replication in vitro by peptide nucleic acids. Nat. Genet. 15, 212–215 (1997).
Article CAS Google Scholar
Nikaido, H. Prevention of drug access to bacterial targets: permeability barriers and active efflux. Science 264, 382–388 (1994).
Article CAS Google Scholar
Good, L., Sandberg, R., Larsson, O., Nielsen, P.E. & Wahlestedt, C. Antisense PNA effects in Escherichia coli are limited by the outer- membrane LPS layer. Microbiology 146, 2665–2670 (2000).
Article CAS Google Scholar
Sekiguchi, M. & Iida, S. Mutants of Escherichia coli permeable to actinomycin. Proc. Natl. Acad. Sci. USA 58, 2315–2320 (1967).
Article CAS Google Scholar
Wagner, R.W., Matteucci, M.D., Grant, D., Huang, T. & Froehler, B.C. Potent and selective inhibition of gene expression by an antisense heptanucleotide. Nat. Biotechnol. 14, 840–844 (1996).
Article CAS Google Scholar
Hancock, R.E. Peptide antibiotics. Lancet 349, 418–422 (1997).
Article CAS Google Scholar
Schwarze, S.R., Hruska, K.A. & Dowdy, S.F. Protein transduction: unrestricted delivery into all cells? Trends Cell Biol. 10, 290–295 (2000).
Article CAS Google Scholar
Aldrian-Herrada, G. et al. A peptide nucleic acid (PNA) is more rapidly internalized in cultured neurons when coupled to a retro-inverso delivery peptide. The antisense activity depresses the target mRNA and protein in magnocellular oxytocin neurons. Nucleic Acids Res. 26, 4910–4916 (1998).
Article CAS Google Scholar
Pooga, M. et al. Cell penetrating PNA constructs regulate galanin receptor levels and modify pain transmission in vivo. Nat. Biotechnol. 16, 857–861 (1998).
Article CAS Google Scholar
Cutrona, G. et al. Effects in live cells of a c-myc anti-gene PNA linked to a nuclear localization signal. Nat. Biotechnol. 18, 300–303 (2000)
Article CAS Google Scholar
Vaara, M. & Porro, M. Group of peptides that act synergistically with hydrophobic antibiotics against gram-negative enteric bacteria. Antimicrob. Agents Chemother. 40, 1801–1805 (1996).
Article CAS Google Scholar
Cronan, J.E. & Rock, C.O. Biosynthesis of membrane lipids. In Escherichia coli and Salmonella: cellular and molecular biology, Edn. 2. (eds Neidhardt, F.C. & Curtiss, R.) 612–636 (American Society for Microbiology, Washington, DC; 1996).
Google Scholar
Christensen, L. et al. Solid-phase synthesis of peptide nucleic acids. J. Peptide Sci. 1, 175–183 (1995).
Article CAS Google Scholar
Miller, J.H. Experiments in molecular genetics. (Cold Spring Harbor Press, Cold Spring Harbor, NY; 1972).
Google Scholar

Download references