A quantitative assay for HIV DNA integration in vivo (original) (raw)

Nature Medicine volume 7, pages 631–634 (2001)Cite this article

Abstract

Early steps of infection by HIV-1 involve entry of the viral core into cells, reverse transcription to form the linear viral DNA, and integration of that DNA into a chromosome of the host. The unintegrated DNA can also follow non-productive pathways, in which it is circularized by recombination between DNA long-terminal repeats (LTRs), circularized by ligation of the DNA ends or degraded. Here we report quantitative methods that monitor formation of reverse transcription products, two-LTR circles and integrated proviruses. The integration assay employs a novel quantitative form of Alu-PCR that should be generally applicable to studies of integrating viruses and gene transfer vectors.

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Acknowledgements

This work was supported by NIH grants GM56553 and AI34786 to F.D.B., the James B. Pendleton Charitable Trust, the Berger Foundation, and Cornelia Mackey.

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Authors and Affiliations

  1. Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California, USA
    Scott L. Butler, Mark S.T. Hansen & Frederic D. Bushman

Authors

  1. Scott L. Butler
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  2. Mark S.T. Hansen
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  3. Frederic D. Bushman
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Corresponding author

Correspondence toFrederic D. Bushman.

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Butler, S., Hansen, M. & Bushman, F. A quantitative assay for HIV DNA integration in vivo.Nat Med 7, 631–634 (2001). https://doi.org/10.1038/87979

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