TP53 mutation analyses on breast carcinomas: a study of paraffin-embedded archival material (original) (raw)

Experimental Oncology
Published: 01 August 1996
L Tryggvadottir,
JG Jonasson,
H Sigurdsson,
K Olafsdottir,
BA Agnarsson,
H Ogmundsdottir &
…
JE Eyfjörd

British Journal of Cancer volume 74, pages 555–561 (1996)Cite this article

173 Accesses
33 Citations
Metrics details

Abstract

The aim of this investigation was to examine the possibility of analysing TP53 mutations in archival paraffin-embedded material with the constant denaturant gel electrophoresis (CDGE) method. We extracted DNA from 193 archival primary breast carcinoma samples, diagnosed in 1981-83; further analysis was possible for 186 of these. TP53 mutations in exons 5-8 were detected with CDGE in 30 samples (16.1%) and 17 of these mutations were confirmed by sequencing. Immunohistochemistry demonstrated TP53 nuclear accumulation in 58 tumours (31%). A strong association between the presence of TP53 mutations and TP53 immunostaining was observed (P < 0.001). Our mutation and immunohistochemistry results are in agreement with other findings based on fresh tumour tissue. TP53 abnormalities were significantly related to high S-phase fraction, low oestrogen receptor (ER) content and high tumour grade. Survival of patients with TP53 abnormalities, in the group as a whole, did not differ from patients with normal TP53. Our study did, however, show that patients with abnormal TP53 had a significantly shorter post-recurrence survival (P = 0.005) than patients with normal TP53.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 24 print issues and online access

$259.00 per year

only $10.79 per issue

Buy this article

Purchase on SpringerLink
Instant access to full article PDF

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Author information

Authors and Affiliations

Molecular and Cell Biology Research Laboratory, Icelandic Cancer Society, Reykjavik, Iceland
S Gretarsdottir

Authors

S Gretarsdottir
L Tryggvadottir
JG Jonasson
H Sigurdsson
K Olafsdottir
H Ogmundsdottir
JE Eyfjörd

Rights and permissions

About this article

Cite this article

Gretarsdottir, S., Tryggvadottir, L., Jonasson, J. et al. TP53 mutation analyses on breast carcinomas: a study of paraffin-embedded archival material.Br J Cancer 74, 555–561 (1996). https://doi.org/10.1038/bjc.1996.400

Download citation

Issue Date: 01 August 1996
DOI: https://doi.org/10.1038/bjc.1996.400