TP53 mutation analyses on breast carcinomas: a study of paraffin-embedded archival material (original) (raw)
- Experimental Oncology
- Published: 01 August 1996
- L Tryggvadottir,
- JG Jonasson,
- H Sigurdsson,
- K Olafsdottir,
- BA Agnarsson,
- H Ogmundsdottir &
- …
- JE Eyfjörd
British Journal of Cancer volume 74, pages 555–561 (1996)Cite this article
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Abstract
The aim of this investigation was to examine the possibility of analysing TP53 mutations in archival paraffin-embedded material with the constant denaturant gel electrophoresis (CDGE) method. We extracted DNA from 193 archival primary breast carcinoma samples, diagnosed in 1981-83; further analysis was possible for 186 of these. TP53 mutations in exons 5-8 were detected with CDGE in 30 samples (16.1%) and 17 of these mutations were confirmed by sequencing. Immunohistochemistry demonstrated TP53 nuclear accumulation in 58 tumours (31%). A strong association between the presence of TP53 mutations and TP53 immunostaining was observed (P < 0.001). Our mutation and immunohistochemistry results are in agreement with other findings based on fresh tumour tissue. TP53 abnormalities were significantly related to high S-phase fraction, low oestrogen receptor (ER) content and high tumour grade. Survival of patients with TP53 abnormalities, in the group as a whole, did not differ from patients with normal TP53. Our study did, however, show that patients with abnormal TP53 had a significantly shorter post-recurrence survival (P = 0.005) than patients with normal TP53.
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Authors and Affiliations
- Molecular and Cell Biology Research Laboratory, Icelandic Cancer Society, Reykjavik, Iceland
S Gretarsdottir
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- S Gretarsdottir
You can also search for this author inPubMed Google Scholar - L Tryggvadottir
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Gretarsdottir, S., Tryggvadottir, L., Jonasson, J. et al. TP53 mutation analyses on breast carcinomas: a study of paraffin-embedded archival material.Br J Cancer 74, 555–561 (1996). https://doi.org/10.1038/bjc.1996.400
- Issue Date: 01 August 1996
- DOI: https://doi.org/10.1038/bjc.1996.400